4CYM

Complex of human VARP-ANKRD1 with Rab32-GppCp

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005739	cellular_component	mitochondrion
A	0005741	cellular_component	mitochondrial outer membrane
A	0005769	cellular_component	early endosome
A	0005783	cellular_component	endoplasmic reticulum
A	0005794	cellular_component	Golgi apparatus
A	0005802	cellular_component	trans-Golgi network
A	0005829	cellular_component	cytosol
A	0006886	biological_process	intracellular protein transport
A	0007005	biological_process	mitochondrion organization
A	0012505	cellular_component	endomembrane system
A	0016020	cellular_component	membrane
A	0016192	biological_process	vesicle-mediated transport
A	0016787	molecular_function	hydrolase activity
A	0019882	biological_process	antigen processing and presentation
A	0030670	cellular_component	phagocytic vesicle membrane
A	0030742	molecular_function	GTP-dependent protein binding
A	0031090	cellular_component	organelle membrane
A	0031410	cellular_component	cytoplasmic vesicle
A	0031905	cellular_component	early endosome lumen
A	0031982	cellular_component	vesicle
A	0032438	biological_process	melanosome organization
A	0033162	cellular_component	melanosome membrane
A	0035612	molecular_function	AP-2 adaptor complex binding
A	0035646	biological_process	endosome to melanosome transport
A	0035650	molecular_function	AP-1 adaptor complex binding
A	0035651	molecular_function	AP-3 adaptor complex binding
A	0036461	molecular_function	BLOC-2 complex binding
A	0042470	cellular_component	melanosome
A	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
A	0045335	cellular_component	phagocytic vesicle
A	0072657	biological_process	protein localization to membrane
A	0090382	biological_process	phagosome maturation
A	1903232	biological_process	melanosome assembly
B	0000166	molecular_function	nucleotide binding
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005739	cellular_component	mitochondrion
B	0005741	cellular_component	mitochondrial outer membrane
B	0005769	cellular_component	early endosome
B	0005783	cellular_component	endoplasmic reticulum
B	0005794	cellular_component	Golgi apparatus
B	0005802	cellular_component	trans-Golgi network
B	0005829	cellular_component	cytosol
B	0006886	biological_process	intracellular protein transport
B	0007005	biological_process	mitochondrion organization
B	0012505	cellular_component	endomembrane system
B	0016020	cellular_component	membrane
B	0016192	biological_process	vesicle-mediated transport
B	0016787	molecular_function	hydrolase activity
B	0019882	biological_process	antigen processing and presentation
B	0030670	cellular_component	phagocytic vesicle membrane
B	0030742	molecular_function	GTP-dependent protein binding
B	0031090	cellular_component	organelle membrane
B	0031410	cellular_component	cytoplasmic vesicle
B	0031905	cellular_component	early endosome lumen
B	0031982	cellular_component	vesicle
B	0032438	biological_process	melanosome organization
B	0033162	cellular_component	melanosome membrane
B	0035612	molecular_function	AP-2 adaptor complex binding
B	0035646	biological_process	endosome to melanosome transport
B	0035650	molecular_function	AP-1 adaptor complex binding
B	0035651	molecular_function	AP-3 adaptor complex binding
B	0036461	molecular_function	BLOC-2 complex binding
B	0042470	cellular_component	melanosome
B	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
B	0045335	cellular_component	phagocytic vesicle
B	0072657	biological_process	protein localization to membrane
B	0090382	biological_process	phagosome maturation
B	1903232	biological_process	melanosome assembly
C	0000166	molecular_function	nucleotide binding
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005739	cellular_component	mitochondrion
C	0005741	cellular_component	mitochondrial outer membrane
C	0005769	cellular_component	early endosome
C	0005783	cellular_component	endoplasmic reticulum
C	0005794	cellular_component	Golgi apparatus
C	0005802	cellular_component	trans-Golgi network
C	0005829	cellular_component	cytosol
C	0006886	biological_process	intracellular protein transport
C	0007005	biological_process	mitochondrion organization
C	0012505	cellular_component	endomembrane system
C	0016020	cellular_component	membrane
C	0016192	biological_process	vesicle-mediated transport
C	0016787	molecular_function	hydrolase activity
C	0019882	biological_process	antigen processing and presentation
C	0030670	cellular_component	phagocytic vesicle membrane
C	0030742	molecular_function	GTP-dependent protein binding
C	0031090	cellular_component	organelle membrane
C	0031410	cellular_component	cytoplasmic vesicle
C	0031905	cellular_component	early endosome lumen
C	0031982	cellular_component	vesicle
C	0032438	biological_process	melanosome organization
C	0033162	cellular_component	melanosome membrane
C	0035612	molecular_function	AP-2 adaptor complex binding
C	0035646	biological_process	endosome to melanosome transport
C	0035650	molecular_function	AP-1 adaptor complex binding
C	0035651	molecular_function	AP-3 adaptor complex binding
C	0036461	molecular_function	BLOC-2 complex binding
C	0042470	cellular_component	melanosome
C	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
C	0045335	cellular_component	phagocytic vesicle
C	0072657	biological_process	protein localization to membrane
C	0090382	biological_process	phagosome maturation
C	1903232	biological_process	melanosome assembly

Functional Information from PDB Data

site_id	AC1
Number of Residues	26
Details	BINDING SITE FOR RESIDUE GCP A 1198

Chain	Residue
A	LEU34
A	GLN52
A	TYR54
A	ALA56
A	THR57
A	GLY84
A	ASN143
A	LYS144
A	ASP146
A	GLN147
A	SER174
A	GLY35
A	ALA175
A	LYS176
A	MG1199
A	HOH2003
A	HOH2004
A	HOH2012
A	HOH2013
A	VAL36
A	GLY37
A	LYS38
A	THR39
A	SER40
A	PHE50
A	SER51

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 1199

Chain	Residue
A	THR39
A	THR57
A	GCP1198
A	HOH2003
A	HOH2004

---

site_id	AC3
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GCP B 1198

Chain	Residue
B	LEU34
B	GLY35
B	VAL36
B	GLY37
B	LYS38
B	THR39
B	SER40
B	PHE50
B	SER51
B	GLN52
B	TYR54
B	ALA56
B	THR57
B	GLY84
B	ASN143
B	LYS144
B	ASP146
B	SER174
B	ALA175
B	LYS176
B	MG1199
B	HOH2003
B	HOH2004
B	HOH2008

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 1199

Chain	Residue
B	THR39
B	THR57
B	GCP1198
B	HOH2003
B	HOH2004

---

site_id	AC5
Number of Residues	27
Details	BINDING SITE FOR RESIDUE GCP C 1198

Chain	Residue
C	GLU33
C	LEU34
C	GLY35
C	VAL36
C	GLY37
C	LYS38
C	THR39
C	SER40
C	PHE50
C	SER51
C	GLN52
C	HIS53
C	TYR54
C	ALA56
C	THR57
C	GLY84
C	ASN143
C	LYS144
C	ASP146
C	GLN147
C	SER174
C	ALA175
C	LYS176
C	MG1199
C	HOH2003
C	HOH2004
C	HOH2006

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 1199

Chain	Residue
C	THR39
C	THR57
C	GCP1198
C	HOH2003
C	HOH2004

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	32
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGVGKTSIIKrYvhqlfsqhyratigvd..FALK

Chain	Residue	Details
A	LEU34-LYS65

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	39
Details	BINDING: BINDING => ECO:0000305|PubMed:24856514, ECO:0007744|PDB:4CYM, ECO:0007744|PDB:4CZ2

Chain	Residue	Details
A	VAL36
A	LYS144
A	ASP146
A	ALA175
A	LYS176
B	VAL36
B	GLY37
B	LYS38
B	SER40
B	SER51
B	GLN52
A	GLY37
B	TYR54
B	GLY84
B	ASN143
B	LYS144
B	ASP146
B	ALA175
B	LYS176
C	VAL36
C	GLY37
C	LYS38
A	LYS38
C	SER40
C	SER51
C	GLN52
C	TYR54
C	GLY84
C	ASN143
C	LYS144
C	ASP146
C	ALA175
C	LYS176
A	SER40
A	SER51
A	GLN52
A	TYR54
A	GLY84
A	ASN143

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:24856514, ECO:0007744|PDB:4CYM, ECO:0007744|PDB:4CZ2

Chain	Residue	Details
A	THR39
A	THR57
B	THR39
B	THR57
C	THR39
C	THR57

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:24856514, ECO:0007744|PDB:4CZ2

Chain	Residue	Details
A	ASP81
B	ASP81
C	ASP81

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:25489052, ECO:0000269|Ref.7, ECO:0007744|PubMed:22223895

Chain	Residue	Details
A	ALA2
B	ALA2
C	ALA2

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER71
B	SER71
C	SER71

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	LIPID: S-geranylgeranyl cysteine => ECO:0000250

Chain	Residue	Details
A	CYS224
A	CYS225
B	CYS224
B	CYS225
C	CYS224
C	CYS225

---