3UWL
Crystal structure of Enteroccocus faecalis thymidylate synthase (EfTS) in complex with 5-formyl tetrahydrofolate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0032259 | biological_process | methylation |
| C | 0004799 | molecular_function | thymidylate synthase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006231 | biological_process | dTMP biosynthetic process |
| C | 0006235 | biological_process | dTTP biosynthetic process |
| C | 0008168 | molecular_function | methyltransferase activity |
| C | 0009165 | biological_process | nucleotide biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| C | 0032259 | biological_process | methylation |
| D | 0004799 | molecular_function | thymidylate synthase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006231 | biological_process | dTMP biosynthetic process |
| D | 0006235 | biological_process | dTTP biosynthetic process |
| D | 0008168 | molecular_function | methyltransferase activity |
| D | 0009165 | biological_process | nucleotide biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| D | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B 317 |
| Chain | Residue |
| A | ARG177 |
| A | ARG178 |
| A | HOH317 |
| B | ARG22 |
| B | CME197 |
| B | ARG217 |
| B | SER218 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 318 |
| Chain | Residue |
| B | ARG177 |
| B | ARG178 |
| B | HOH403 |
| A | ARG217 |
| A | SER218 |
| site_id | AC3 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE FOZ B 316 |
| Chain | Residue |
| B | LEU55 |
| B | ILE80 |
| B | TRP81 |
| B | TRP84 |
| B | LEU194 |
| B | CME197 |
| B | ASP220 |
| B | LEU223 |
| B | GLY224 |
| B | PHE227 |
| B | ASN228 |
| B | ILE313 |
| B | ALA314 |
| B | HOH344 |
| B | HOH358 |
| B | HOH361 |
| B | HOH365 |
| B | HOH416 |
| B | HOH431 |
| B | HOH434 |
| B | HOH435 |
| B | HOH439 |
| B | HOH440 |
| B | HOH511 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 319 |
| Chain | Residue |
| B | ARG50 |
| B | PRO307 |
| B | THR308 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO B 320 |
| Chain | Residue |
| B | GLU168 |
| B | GLU242 |
| B | SER289 |
| B | PHE291 |
| B | HOH443 |
| D | HOH422 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO B 321 |
| Chain | Residue |
| B | GLU3 |
| B | ALA7 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO B 322 |
| Chain | Residue |
| B | PHE18 |
| B | TYR28 |
| B | GLN261 |
| D | GLN268 |
| D | HOH741 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO B 323 |
| Chain | Residue |
| B | LEU13 |
| B | TYR28 |
| B | HOH409 |
| D | LEU13 |
| D | TYR28 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO C 318 |
| Chain | Residue |
| C | ALA4 |
| C | MET35 |
| C | ARG36 |
| C | HOH773 |
| C | HOH795 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE EDO C 319 |
| Chain | Residue |
| C | LEU13 |
| C | TYR28 |
| C | HOH374 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 317 |
| Chain | Residue |
| C | LEU194 |
| C | ARG217 |
| C | SER218 |
| C | HOH329 |
| D | ARG177 |
| D | ARG178 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 D 317 |
| Chain | Residue |
| C | ARG177 |
| C | ARG178 |
| D | ARG22 |
| D | CME197 |
| D | ARG217 |
| D | SER218 |
| D | HOH374 |
| site_id | BC4 |
| Number of Residues | 27 |
| Details | BINDING SITE FOR RESIDUE FOZ D 316 |
| Chain | Residue |
| D | HOH416 |
| D | HOH418 |
| D | HOH431 |
| D | HOH463 |
| D | HOH576 |
| D | HOH614 |
| D | LEU55 |
| D | ILE80 |
| D | TRP81 |
| D | TRP84 |
| D | LEU194 |
| D | CME197 |
| D | ASP220 |
| D | LEU223 |
| D | GLY224 |
| D | PHE227 |
| D | ASN228 |
| D | ILE313 |
| D | ALA314 |
| D | HOH344 |
| D | HOH349 |
| D | HOH351 |
| D | HOH381 |
| D | HOH385 |
| D | HOH392 |
| D | HOH403 |
| D | HOH410 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 318 |
| Chain | Residue |
| D | ARG50 |
| D | PRO307 |
| D | THR308 |
| site_id | BC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO D 320 |
| Chain | Residue |
| B | HOH384 |
| D | ALA164 |
| D | GLU168 |
| D | GLU242 |
| D | SER289 |
| D | PHE291 |
| D | HOH424 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO D 321 |
| Chain | Residue |
| D | ARG76 |
| D | ASP294 |
| D | MET295 |
| D | HOH388 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RrlIvsaWNpedvpsma.....LpPCHtmfQFyV |
| Chain | Residue | Details |
| B | ARG177-VAL205 | |
| A | ARG177-VAL205 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_00008","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 28 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"HAMAP-Rule","id":"MF_00008","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00008","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
