3U6K
Ef-tu (escherichia coli) in complex with nvp-ldk733
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003746 | molecular_function | translation elongation factor activity |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006412 | biological_process | translation |
| A | 0006414 | biological_process | translational elongation |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0032045 | cellular_component | guanyl-nucleotide exchange factor complex |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003746 | molecular_function | translation elongation factor activity |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005525 | molecular_function | GTP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006412 | biological_process | translation |
| B | 0006414 | biological_process | translational elongation |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0032045 | cellular_component | guanyl-nucleotide exchange factor complex |
| B | 0046677 | biological_process | response to antibiotic |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0097216 | molecular_function | guanosine tetraphosphate binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE GDP A 500 |
| Chain | Residue |
| A | ASP21 |
| A | ASP138 |
| A | MET139 |
| A | SER173 |
| A | ALA174 |
| A | LEU175 |
| A | MG394 |
| A | HOH439 |
| A | HOH441 |
| A | HOH444 |
| B | ASP47 |
| A | HIS22 |
| B | ASN51 |
| A | GLY23 |
| A | LYS24 |
| A | THR25 |
| A | THR26 |
| A | PHE46 |
| A | ASN135 |
| A | LYS136 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MG A 394 |
| Chain | Residue |
| A | THR25 |
| A | CYS81 |
| A | HOH439 |
| A | HOH441 |
| A | HOH442 |
| A | HOH444 |
| A | GDP500 |
| site_id | AC3 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE GDP B 500 |
| Chain | Residue |
| B | HIS19 |
| B | ASP21 |
| B | HIS22 |
| B | GLY23 |
| B | LYS24 |
| B | THR25 |
| B | THR26 |
| B | PHE46 |
| B | ASN135 |
| B | LYS136 |
| B | ASP138 |
| B | MET139 |
| B | ALA174 |
| B | MG394 |
| B | HOH395 |
| B | HOH396 |
| B | HOH403 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 394 |
| Chain | Residue |
| B | THR25 |
| B | HOH395 |
| B | HOH396 |
| B | HOH397 |
| B | HOH403 |
| B | GDP500 |
| site_id | AC5 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR CHAIN C OF NVP-LDK733 |
| Chain | Residue |
| A | GLU215 |
| A | ASP216 |
| A | PHE218 |
| A | ILE220 |
| A | THR228 |
| A | THR256 |
| A | GLY257 |
| A | GLU259 |
| A | PHE261 |
| A | ARG262 |
| A | ASN273 |
| A | VAL274 |
| A | GLY275 |
| A | LEU277 |
| A | ARG381 |
| A | HOH402 |
| B | THR38 |
| B | TYR39 |
| B | ARG318 |
| B | THR320 |
| B | PRO321 |
| B | PHE323 |
| B | HOH423 |
| C | HOH18 |
| site_id | AC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR CHAIN D OF NVP-LDK733 |
| Chain | Residue |
| A | TYR39 |
| A | PRO72 |
| A | THR320 |
| A | PRO321 |
| A | PHE323 |
| A | TYR326 |
| A | HOH448 |
| B | GLU215 |
| B | ASP216 |
| B | ARG223 |
| B | THR228 |
| B | GLY257 |
| B | VAL258 |
| B | GLU259 |
| B | PHE261 |
| B | ASN273 |
| B | VAL274 |
| B | GLY275 |
| B | LEU277 |
| B | HOH447 |
| D | HOH13 |
| D | HOH143 |
Functional Information from PROSITE/UniProt
| site_id | PS00301 |
| Number of Residues | 16 |
| Details | G_TR_1 Translational (tr)-type guanine nucleotide-binding (G) domain signature. DNapeEKaRGITIntS |
| Chain | Residue | Details |
| A | ASP50-SER65 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 388 |
| Details | Domain: {"description":"tr-type G"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 14 |
| Details | Region: {"description":"G1","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Region: {"description":"G2","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | Region: {"description":"G3","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | Region: {"description":"G4","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Region: {"description":"G5","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 18 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"29546243","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5OPD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"29546243","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5MI3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5OPD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-methyllysine; alternate","evidences":[{"source":"PubMed","id":"2022614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"389663","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"6997043","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7021545","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19150849","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24141193","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8416965","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N4-methylasparagine","evidences":[{"source":"PubMed","id":"7592050","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 535 |
| Chain | Residue | Details |
| A | ASP21 | electrostatic stabiliser |
| A | LYS24 | electrostatic stabiliser |
| A | THR25 | metal ligand |
| A | HIS84 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 535 |
| Chain | Residue | Details |
| B | ASP21 | electrostatic stabiliser |
| B | LYS24 | electrostatic stabiliser |
| B | THR25 | metal ligand |
| B | HIS84 | electrostatic stabiliser |
