3U6K

Ef-tu (escherichia coli) in complex with nvp-ldk733

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-20
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	80.372, 82.216, 129.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.040 - 2.450
R-factor	0.228
Rwork	0.224
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	32122
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 HEPES PH 7.5, 0.3 MGCL2, 21% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K