3SBC

Crystal structure of Saccharomyces cerevisiae TSA1C47S mutant protein

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000077	biological_process	DNA damage checkpoint signaling
A	0004601	molecular_function	peroxidase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006111	biological_process	regulation of gluconeogenesis
A	0006457	biological_process	protein folding
A	0006979	biological_process	response to oxidative stress
A	0008379	molecular_function	thioredoxin peroxidase activity
A	0016209	molecular_function	antioxidant activity
A	0016491	molecular_function	oxidoreductase activity
A	0019207	molecular_function	kinase regulator activity
A	0033194	biological_process	response to hydroperoxide
A	0034599	biological_process	cellular response to oxidative stress
A	0034605	biological_process	cellular response to heat
A	0042262	biological_process	DNA protection
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0042802	molecular_function	identical protein binding
A	0043022	molecular_function	ribosome binding
A	0045454	biological_process	cell redox homeostasis
A	0050821	biological_process	protein stabilization
A	0051082	molecular_function	unfolded protein binding
A	0051258	biological_process	protein polymerization
A	0051920	molecular_function	peroxiredoxin activity
A	0071447	biological_process	cellular response to hydroperoxide
A	0072721	biological_process	cellular response to dithiothreitol
A	0098869	biological_process	cellular oxidant detoxification
A	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
B	0000077	biological_process	DNA damage checkpoint signaling
B	0004601	molecular_function	peroxidase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006111	biological_process	regulation of gluconeogenesis
B	0006457	biological_process	protein folding
B	0006979	biological_process	response to oxidative stress
B	0008379	molecular_function	thioredoxin peroxidase activity
B	0016209	molecular_function	antioxidant activity
B	0016491	molecular_function	oxidoreductase activity
B	0019207	molecular_function	kinase regulator activity
B	0033194	biological_process	response to hydroperoxide
B	0034599	biological_process	cellular response to oxidative stress
B	0034605	biological_process	cellular response to heat
B	0042262	biological_process	DNA protection
B	0042744	biological_process	hydrogen peroxide catabolic process
B	0042802	molecular_function	identical protein binding
B	0043022	molecular_function	ribosome binding
B	0045454	biological_process	cell redox homeostasis
B	0050821	biological_process	protein stabilization
B	0051082	molecular_function	unfolded protein binding
B	0051258	biological_process	protein polymerization
B	0051920	molecular_function	peroxiredoxin activity
B	0071447	biological_process	cellular response to hydroperoxide
B	0072721	biological_process	cellular response to dithiothreitol
B	0098869	biological_process	cellular oxidant detoxification
B	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
C	0000077	biological_process	DNA damage checkpoint signaling
C	0004601	molecular_function	peroxidase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006111	biological_process	regulation of gluconeogenesis
C	0006457	biological_process	protein folding
C	0006979	biological_process	response to oxidative stress
C	0008379	molecular_function	thioredoxin peroxidase activity
C	0016209	molecular_function	antioxidant activity
C	0016491	molecular_function	oxidoreductase activity
C	0019207	molecular_function	kinase regulator activity
C	0033194	biological_process	response to hydroperoxide
C	0034599	biological_process	cellular response to oxidative stress
C	0034605	biological_process	cellular response to heat
C	0042262	biological_process	DNA protection
C	0042744	biological_process	hydrogen peroxide catabolic process
C	0042802	molecular_function	identical protein binding
C	0043022	molecular_function	ribosome binding
C	0045454	biological_process	cell redox homeostasis
C	0050821	biological_process	protein stabilization
C	0051082	molecular_function	unfolded protein binding
C	0051258	biological_process	protein polymerization
C	0051920	molecular_function	peroxiredoxin activity
C	0071447	biological_process	cellular response to hydroperoxide
C	0072721	biological_process	cellular response to dithiothreitol
C	0098869	biological_process	cellular oxidant detoxification
C	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
D	0000077	biological_process	DNA damage checkpoint signaling
D	0004601	molecular_function	peroxidase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006111	biological_process	regulation of gluconeogenesis
D	0006457	biological_process	protein folding
D	0006979	biological_process	response to oxidative stress
D	0008379	molecular_function	thioredoxin peroxidase activity
D	0016209	molecular_function	antioxidant activity
D	0016491	molecular_function	oxidoreductase activity
D	0019207	molecular_function	kinase regulator activity
D	0033194	biological_process	response to hydroperoxide
D	0034599	biological_process	cellular response to oxidative stress
D	0034605	biological_process	cellular response to heat
D	0042262	biological_process	DNA protection
D	0042744	biological_process	hydrogen peroxide catabolic process
D	0042802	molecular_function	identical protein binding
D	0043022	molecular_function	ribosome binding
D	0045454	biological_process	cell redox homeostasis
D	0050821	biological_process	protein stabilization
D	0051082	molecular_function	unfolded protein binding
D	0051258	biological_process	protein polymerization
D	0051920	molecular_function	peroxiredoxin activity
D	0071447	biological_process	cellular response to hydroperoxide
D	0072721	biological_process	cellular response to dithiothreitol
D	0098869	biological_process	cellular oxidant detoxification
D	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
E	0000077	biological_process	DNA damage checkpoint signaling
E	0004601	molecular_function	peroxidase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006111	biological_process	regulation of gluconeogenesis
E	0006457	biological_process	protein folding
E	0006979	biological_process	response to oxidative stress
E	0008379	molecular_function	thioredoxin peroxidase activity
E	0016209	molecular_function	antioxidant activity
E	0016491	molecular_function	oxidoreductase activity
E	0019207	molecular_function	kinase regulator activity
E	0033194	biological_process	response to hydroperoxide
E	0034599	biological_process	cellular response to oxidative stress
E	0034605	biological_process	cellular response to heat
E	0042262	biological_process	DNA protection
E	0042744	biological_process	hydrogen peroxide catabolic process
E	0042802	molecular_function	identical protein binding
E	0043022	molecular_function	ribosome binding
E	0045454	biological_process	cell redox homeostasis
E	0050821	biological_process	protein stabilization
E	0051082	molecular_function	unfolded protein binding
E	0051258	biological_process	protein polymerization
E	0051920	molecular_function	peroxiredoxin activity
E	0071447	biological_process	cellular response to hydroperoxide
E	0072721	biological_process	cellular response to dithiothreitol
E	0098869	biological_process	cellular oxidant detoxification
E	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
F	0000077	biological_process	DNA damage checkpoint signaling
F	0004601	molecular_function	peroxidase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006111	biological_process	regulation of gluconeogenesis
F	0006457	biological_process	protein folding
F	0006979	biological_process	response to oxidative stress
F	0008379	molecular_function	thioredoxin peroxidase activity
F	0016209	molecular_function	antioxidant activity
F	0016491	molecular_function	oxidoreductase activity
F	0019207	molecular_function	kinase regulator activity
F	0033194	biological_process	response to hydroperoxide
F	0034599	biological_process	cellular response to oxidative stress
F	0034605	biological_process	cellular response to heat
F	0042262	biological_process	DNA protection
F	0042744	biological_process	hydrogen peroxide catabolic process
F	0042802	molecular_function	identical protein binding
F	0043022	molecular_function	ribosome binding
F	0045454	biological_process	cell redox homeostasis
F	0050821	biological_process	protein stabilization
F	0051082	molecular_function	unfolded protein binding
F	0051258	biological_process	protein polymerization
F	0051920	molecular_function	peroxiredoxin activity
F	0071447	biological_process	cellular response to hydroperoxide
F	0072721	biological_process	cellular response to dithiothreitol
F	0098869	biological_process	cellular oxidant detoxification
F	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
G	0000077	biological_process	DNA damage checkpoint signaling
G	0004601	molecular_function	peroxidase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006111	biological_process	regulation of gluconeogenesis
G	0006457	biological_process	protein folding
G	0006979	biological_process	response to oxidative stress
G	0008379	molecular_function	thioredoxin peroxidase activity
G	0016209	molecular_function	antioxidant activity
G	0016491	molecular_function	oxidoreductase activity
G	0019207	molecular_function	kinase regulator activity
G	0033194	biological_process	response to hydroperoxide
G	0034599	biological_process	cellular response to oxidative stress
G	0034605	biological_process	cellular response to heat
G	0042262	biological_process	DNA protection
G	0042744	biological_process	hydrogen peroxide catabolic process
G	0042802	molecular_function	identical protein binding
G	0043022	molecular_function	ribosome binding
G	0045454	biological_process	cell redox homeostasis
G	0050821	biological_process	protein stabilization
G	0051082	molecular_function	unfolded protein binding
G	0051258	biological_process	protein polymerization
G	0051920	molecular_function	peroxiredoxin activity
G	0071447	biological_process	cellular response to hydroperoxide
G	0072721	biological_process	cellular response to dithiothreitol
G	0098869	biological_process	cellular oxidant detoxification
G	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
H	0000077	biological_process	DNA damage checkpoint signaling
H	0004601	molecular_function	peroxidase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006111	biological_process	regulation of gluconeogenesis
H	0006457	biological_process	protein folding
H	0006979	biological_process	response to oxidative stress
H	0008379	molecular_function	thioredoxin peroxidase activity
H	0016209	molecular_function	antioxidant activity
H	0016491	molecular_function	oxidoreductase activity
H	0019207	molecular_function	kinase regulator activity
H	0033194	biological_process	response to hydroperoxide
H	0034599	biological_process	cellular response to oxidative stress
H	0034605	biological_process	cellular response to heat
H	0042262	biological_process	DNA protection
H	0042744	biological_process	hydrogen peroxide catabolic process
H	0042802	molecular_function	identical protein binding
H	0043022	molecular_function	ribosome binding
H	0045454	biological_process	cell redox homeostasis
H	0050821	biological_process	protein stabilization
H	0051082	molecular_function	unfolded protein binding
H	0051258	biological_process	protein polymerization
H	0051920	molecular_function	peroxiredoxin activity
H	0071447	biological_process	cellular response to hydroperoxide
H	0072721	biological_process	cellular response to dithiothreitol
H	0098869	biological_process	cellular oxidant detoxification
H	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
I	0000077	biological_process	DNA damage checkpoint signaling
I	0004601	molecular_function	peroxidase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006111	biological_process	regulation of gluconeogenesis
I	0006457	biological_process	protein folding
I	0006979	biological_process	response to oxidative stress
I	0008379	molecular_function	thioredoxin peroxidase activity
I	0016209	molecular_function	antioxidant activity
I	0016491	molecular_function	oxidoreductase activity
I	0019207	molecular_function	kinase regulator activity
I	0033194	biological_process	response to hydroperoxide
I	0034599	biological_process	cellular response to oxidative stress
I	0034605	biological_process	cellular response to heat
I	0042262	biological_process	DNA protection
I	0042744	biological_process	hydrogen peroxide catabolic process
I	0042802	molecular_function	identical protein binding
I	0043022	molecular_function	ribosome binding
I	0045454	biological_process	cell redox homeostasis
I	0050821	biological_process	protein stabilization
I	0051082	molecular_function	unfolded protein binding
I	0051258	biological_process	protein polymerization
I	0051920	molecular_function	peroxiredoxin activity
I	0071447	biological_process	cellular response to hydroperoxide
I	0072721	biological_process	cellular response to dithiothreitol
I	0098869	biological_process	cellular oxidant detoxification
I	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity
J	0000077	biological_process	DNA damage checkpoint signaling
J	0004601	molecular_function	peroxidase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006111	biological_process	regulation of gluconeogenesis
J	0006457	biological_process	protein folding
J	0006979	biological_process	response to oxidative stress
J	0008379	molecular_function	thioredoxin peroxidase activity
J	0016209	molecular_function	antioxidant activity
J	0016491	molecular_function	oxidoreductase activity
J	0019207	molecular_function	kinase regulator activity
J	0033194	biological_process	response to hydroperoxide
J	0034599	biological_process	cellular response to oxidative stress
J	0034605	biological_process	cellular response to heat
J	0042262	biological_process	DNA protection
J	0042744	biological_process	hydrogen peroxide catabolic process
J	0042802	molecular_function	identical protein binding
J	0043022	molecular_function	ribosome binding
J	0045454	biological_process	cell redox homeostasis
J	0050821	biological_process	protein stabilization
J	0051082	molecular_function	unfolded protein binding
J	0051258	biological_process	protein polymerization
J	0051920	molecular_function	peroxiredoxin activity
J	0071447	biological_process	cellular response to hydroperoxide
J	0072721	biological_process	cellular response to dithiothreitol
J	0098869	biological_process	cellular oxidant detoxification
J	0140824	molecular_function	thioredoxin-dependent peroxiredoxin activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE DTU B 201

Chain	Residue
B	PRO40
B	THR44
B	PHE45
B	VAL46
B	SER47
B	ARG123

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE DTV C 201

Chain	Residue
C	VAL46
C	SER47
C	ARG123
C	LEU142
C	PRO40
C	THR44
C	PHE45

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE DTU E 201

Chain	Residue
E	LEU41
E	THR44
E	PHE45
E	VAL46
E	SER47
E	ARG123
E	LEU142

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE DTU G 201

Chain	Residue
G	THR44
G	PHE45
G	VAL46
G	SER47
G	ARG123
G	LEU142
H	VAL167

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE DTU H 201

Chain	Residue
G	VAL167
H	PRO40
H	THR44
H	PHE45
H	VAL46
H	SER47
H	ARG123
H	HOH322

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DTU J 201

Chain	Residue
A	TYR77
J	THR44
J	PHE45
J	VAL46
J	ARG123

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1580
Details	Domain: {"description":"Thioredoxin","evidences":[{"source":"PROSITE-ProRule","id":"PRU00691","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Active site: {"description":"Cysteine sulfenic acid (-SOH) intermediate","evidences":[{"source":"PubMed","id":"8041739","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	30
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22985967","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3SBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	10
Details	Site: {"description":"Important for catalytic activity, helps activating the peroxidatic cysteine through H-bonding"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	10
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	40
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---