Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3OB7

Human Thymidylate Synthase R163K with Cys 195 covalently modified by Glutathione

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0004799	molecular_function	thymidylate synthase activity
A	0005542	molecular_function	folic acid binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0006231	biological_process	dTMP biosynthetic process
A	0006235	biological_process	dTTP biosynthetic process
A	0008168	molecular_function	methyltransferase activity
A	0009165	biological_process	nucleotide biosynthetic process
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0017148	biological_process	negative regulation of translation
A	0032259	biological_process	methylation
A	0035999	biological_process	tetrahydrofolate interconversion
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0071897	biological_process	DNA biosynthetic process
A	1990825	molecular_function	sequence-specific mRNA binding
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0004799	molecular_function	thymidylate synthase activity
B	0005542	molecular_function	folic acid binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005759	cellular_component	mitochondrial matrix
B	0005829	cellular_component	cytosol
B	0006231	biological_process	dTMP biosynthetic process
B	0006235	biological_process	dTTP biosynthetic process
B	0008168	molecular_function	methyltransferase activity
B	0009165	biological_process	nucleotide biosynthetic process
B	0016020	cellular_component	membrane
B	0016740	molecular_function	transferase activity
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0017148	biological_process	negative regulation of translation
B	0032259	biological_process	methylation
B	0035999	biological_process	tetrahydrofolate interconversion
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0071897	biological_process	DNA biosynthetic process
B	1990825	molecular_function	sequence-specific mRNA binding
C	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
C	0004799	molecular_function	thymidylate synthase activity
C	0005542	molecular_function	folic acid binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005759	cellular_component	mitochondrial matrix
C	0005829	cellular_component	cytosol
C	0006231	biological_process	dTMP biosynthetic process
C	0006235	biological_process	dTTP biosynthetic process
C	0008168	molecular_function	methyltransferase activity
C	0009165	biological_process	nucleotide biosynthetic process
C	0016020	cellular_component	membrane
C	0016740	molecular_function	transferase activity
C	0016741	molecular_function	transferase activity, transferring one-carbon groups
C	0017148	biological_process	negative regulation of translation
C	0032259	biological_process	methylation
C	0035999	biological_process	tetrahydrofolate interconversion
C	0046653	biological_process	tetrahydrofolate metabolic process
C	0071897	biological_process	DNA biosynthetic process
C	1990825	molecular_function	sequence-specific mRNA binding
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0004799	molecular_function	thymidylate synthase activity
D	0005542	molecular_function	folic acid binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005759	cellular_component	mitochondrial matrix
D	0005829	cellular_component	cytosol
D	0006231	biological_process	dTMP biosynthetic process
D	0006235	biological_process	dTTP biosynthetic process
D	0008168	molecular_function	methyltransferase activity
D	0009165	biological_process	nucleotide biosynthetic process
D	0016020	cellular_component	membrane
D	0016740	molecular_function	transferase activity
D	0016741	molecular_function	transferase activity, transferring one-carbon groups
D	0017148	biological_process	negative regulation of translation
D	0032259	biological_process	methylation
D	0035999	biological_process	tetrahydrofolate interconversion
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0071897	biological_process	DNA biosynthetic process
D	1990825	molecular_function	sequence-specific mRNA binding
E	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
E	0004799	molecular_function	thymidylate synthase activity
E	0005542	molecular_function	folic acid binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005759	cellular_component	mitochondrial matrix
E	0005829	cellular_component	cytosol
E	0006231	biological_process	dTMP biosynthetic process
E	0006235	biological_process	dTTP biosynthetic process
E	0008168	molecular_function	methyltransferase activity
E	0009165	biological_process	nucleotide biosynthetic process
E	0016020	cellular_component	membrane
E	0016740	molecular_function	transferase activity
E	0016741	molecular_function	transferase activity, transferring one-carbon groups
E	0017148	biological_process	negative regulation of translation
E	0032259	biological_process	methylation
E	0035999	biological_process	tetrahydrofolate interconversion
E	0046653	biological_process	tetrahydrofolate metabolic process
E	0071897	biological_process	DNA biosynthetic process
E	1990825	molecular_function	sequence-specific mRNA binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GSH A 581

Chain	Residue
A	ARG50
B	ARG175
B	ARG176
A	CYS195
A	HIS196
A	ARG215
A	SER216
A	ASP218
A	GLY222
A	PHE225
A	ASN226

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 A 365

Chain	Residue
A	ARG175
A	ARG176
B	ARG50
B	ARG215
B	SER216

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 C 365

Chain	Residue
C	ARG175
C	ARG176
C	ARG215

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GSH D 314

Chain	Residue
D	ARG50
D	CYS195
D	GLN214
D	ARG215
D	SER216
D	ASP218
D	GLY222
D	PHE225
D	ASN226
E	ARG175
E	ARG176

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 E 366

Chain	Residue
D	ARG175
D	ARG176
E	ARG50
E	PRO193
E	ARG215

Functional Information from PROSITE/UniProt

site_id	PS00091
Number of Residues	29
Details	THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV

Chain	Residue	Details
A	ARG175-VAL203

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	ACT_SITE: Nucleophile => ECO:0000250\|UniProtKB:P0A884

Chain	Residue	Details
A	CYS195
B	CYS195
C	CYS195
D	CYS195
E	CYS195

site_id	SWS_FT_FI2
Number of Residues	10
Details	BINDING: in other chain => ECO:0000250\|UniProtKB:P0A884

Chain	Residue	Details
A	ARG50
E	ASN226
A	ASN226
B	ARG50
B	ASN226
C	ARG50
C	ASN226
D	ARG50
D	ASN226
E	ARG50

site_id	SWS_FT_FI3
Number of Residues	5
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P45352

Chain	Residue	Details
A	ARG175
B	ARG175
C	ARG175
D	ARG175
E	ARG175

site_id	SWS_FT_FI4
Number of Residues	15
Details	BINDING: in other chain => ECO:0000250\|UniProtKB:P45352

Chain	Residue	Details
A	CYS195
D	CYS195
D	ARG215
D	HIS256
E	CYS195
E	ARG215
E	HIS256
A	ARG215
A	HIS256
B	CYS195
B	ARG215
B	HIS256
C	CYS195
C	ARG215
C	HIS256

site_id	SWS_FT_FI5
Number of Residues	10
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P0A884

Chain	Residue	Details
A	ASP218
E	ALA312
A	ALA312
B	ASP218
B	ALA312
C	ASP218
C	ALA312
D	ASP218
D	ALA312
E	ASP218

site_id	SWS_FT_FI6
Number of Residues	5
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER114
B	SER114
C	SER114
D	SER114
E	SER114

site_id	SWS_FT_FI7
Number of Residues	20
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
A	LYS287
C	LYS292
C	LYS308
D	LYS287
D	LYS292
D	LYS308
E	LYS287
E	LYS292
E	LYS308
A	LYS292
A	LYS308
B	LYS287
B	LYS292
B	LYS308
C	LYS287

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)