3NC0

Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal II)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000054	biological_process	ribosomal subunit export from nucleus
A	0000055	biological_process	ribosomal large subunit export from nucleus
A	0000056	biological_process	ribosomal small subunit export from nucleus
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000776	cellular_component	kinetochore
A	0003723	molecular_function	RNA binding
A	0005049	molecular_function	nuclear export signal receptor activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005642	cellular_component	annulate lamellae
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006406	biological_process	mRNA export from nucleus
A	0006611	biological_process	protein export from nucleus
A	0006886	biological_process	intracellular protein transport
A	0006913	biological_process	nucleocytoplasmic transport
A	0009410	biological_process	response to xenobiotic stimulus
A	0010824	biological_process	regulation of centrosome duplication
A	0015030	cellular_component	Cajal body
A	0015031	biological_process	protein transport
A	0019904	molecular_function	protein domain specific binding
A	0031267	molecular_function	small GTPase binding
A	0031965	cellular_component	nuclear membrane
A	0032434	biological_process	regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0032991	cellular_component	protein-containing complex
A	0034504	biological_process	protein localization to nucleus
A	0042176	biological_process	regulation of protein catabolic process
A	0042254	biological_process	ribosome biogenesis
A	0043231	cellular_component	intracellular membrane-bounded organelle
A	0046825	biological_process	regulation of protein export from nucleus
A	0051028	biological_process	mRNA transport
A	0071401	biological_process	cellular response to triglyceride
A	0140297	molecular_function	DNA-binding transcription factor binding
A	1902075	biological_process	cellular response to salt
A	1990904	cellular_component	ribonucleoprotein complex
B	0000339	molecular_function	RNA cap binding
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005643	cellular_component	nuclear pore
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006404	biological_process	RNA import into nucleus
B	0006606	biological_process	protein import into nucleus
B	0007010	biological_process	cytoskeleton organization
B	0042564	cellular_component	NLS-dependent protein nuclear import complex
B	0051259	biological_process	protein complex oligomerization
B	0051262	biological_process	protein tetramerization
B	0061015	biological_process	snRNA import into nucleus
B	0061608	molecular_function	nuclear import signal receptor activity
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0006913	biological_process	nucleocytoplasmic transport
D	0000054	biological_process	ribosomal subunit export from nucleus
D	0000055	biological_process	ribosomal large subunit export from nucleus
D	0000056	biological_process	ribosomal small subunit export from nucleus
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0000776	cellular_component	kinetochore
D	0003723	molecular_function	RNA binding
D	0005049	molecular_function	nuclear export signal receptor activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005642	cellular_component	annulate lamellae
D	0005654	cellular_component	nucleoplasm
D	0005730	cellular_component	nucleolus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006406	biological_process	mRNA export from nucleus
D	0006611	biological_process	protein export from nucleus
D	0006886	biological_process	intracellular protein transport
D	0006913	biological_process	nucleocytoplasmic transport
D	0009410	biological_process	response to xenobiotic stimulus
D	0010824	biological_process	regulation of centrosome duplication
D	0015030	cellular_component	Cajal body
D	0015031	biological_process	protein transport
D	0019904	molecular_function	protein domain specific binding
D	0031267	molecular_function	small GTPase binding
D	0031965	cellular_component	nuclear membrane
D	0032434	biological_process	regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0032991	cellular_component	protein-containing complex
D	0034504	biological_process	protein localization to nucleus
D	0042176	biological_process	regulation of protein catabolic process
D	0042254	biological_process	ribosome biogenesis
D	0043231	cellular_component	intracellular membrane-bounded organelle
D	0046825	biological_process	regulation of protein export from nucleus
D	0051028	biological_process	mRNA transport
D	0071401	biological_process	cellular response to triglyceride
D	0140297	molecular_function	DNA-binding transcription factor binding
D	1902075	biological_process	cellular response to salt
D	1990904	cellular_component	ribonucleoprotein complex
E	0000339	molecular_function	RNA cap binding
E	0003723	molecular_function	RNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005643	cellular_component	nuclear pore
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006404	biological_process	RNA import into nucleus
E	0006606	biological_process	protein import into nucleus
E	0007010	biological_process	cytoskeleton organization
E	0042564	cellular_component	NLS-dependent protein nuclear import complex
E	0051259	biological_process	protein complex oligomerization
E	0051262	biological_process	protein tetramerization
E	0061015	biological_process	snRNA import into nucleus
E	0061608	molecular_function	nuclear import signal receptor activity
F	0003924	molecular_function	GTPase activity
F	0005525	molecular_function	GTP binding
F	0006913	biological_process	nucleocytoplasmic transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL A 1072

Chain	Residue
A	THR65

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 1073

Chain	Residue
A	ASN234
A	GLU270
A	HOH1301

---

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE PEG A 1075

Chain	Residue
A	PHE386

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG A 1076

Chain	Residue
A	GLU655
A	GOL1079

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG A 1077

Chain	Residue
A	GLY526
A	LYS522
A	LEU525

---

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG A 1078

Chain	Residue
A	HOH1128
B	THR297

---

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL A 1079

Chain	Residue
A	PEG1076

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE IPH B 361

Chain	Residue
B	HIS349
D	ALA730
D	GLN733

---

site_id	AC9
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GTP C 217

Chain	Residue
C	GLY20
C	THR21
C	GLY22
C	LYS23
C	THR24
C	THR25
C	GLU36
C	LYS37
C	LYS38
C	TYR39
C	ALA41
C	THR42
C	GLY68
C	ASN122
C	LYS123
C	ASP125
C	ILE126
C	SER150
C	ALA151
C	LYS152
C	HOH193
C	MG218
C	HOH276
C	HOH523

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 218

Chain	Residue
C	THR24
C	THR42
C	ASP65
C	THR66
C	GTP217

---

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL C 181

Chain	Residue
C	LEU43
C	GLU46

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG C 182

Chain	Residue
C	GLU70
C	ARG76
C	HOH495

---

site_id	BC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL D 1072

Chain	Residue
D	HOH1245

---

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG D 1073

Chain	Residue
D	ARG697
D	LYS700
D	HOH1225

---

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PEG D 1074

Chain	Residue
D	CYS34
D	LEU35
D	TYR36
D	GLY38
D	ALA41
F	TRP64

---

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG D 1075

Chain	Residue
D	SER760
D	GLY761
D	SER764
D	ARG765

---

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG D 1076

Chain	Residue
D	ASP436
D	ARG746
D	ARG753

---

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG D 1077

Chain	Residue
D	ASN234
D	TRP235

---

site_id	CC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG D 1078

Chain	Residue
D	LYS144
D	HIS145

---

site_id	CC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL D 1079

Chain	Residue
D	GLU361

---

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL E 361

Chain	Residue
E	THR21
E	LEU104
E	SER105
E	ASP255
E	TRP276

---

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL E 362

Chain	Residue
E	ARG136
E	GLY137
E	ARG161
E	ASN163
E	THR165

---

site_id	CC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE GTP F 217

Chain	Residue
F	ASP125
F	ILE126
F	SER150
F	ALA151
F	LYS152
F	MG218
F	ASP18
F	GLY19
F	GLY20
F	THR21
F	GLY22
F	LYS23
F	THR24
F	THR25
F	GLU36
F	LYS37
F	LYS38
F	TYR39
F	ALA41
F	THR42
F	GLY68
F	ASN122
F	LYS123

---

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 218

Chain	Residue
F	THR24
F	THR42
F	ASP65
F	THR66
F	GTP217

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10078529, ECO:0000269|PubMed:10353245, ECO:0000269|PubMed:10367892, ECO:0000269|PubMed:11832950, ECO:0000269|PubMed:18611384, ECO:0000269|PubMed:19389996, ECO:0000269|PubMed:19505478, ECO:0000269|PubMed:24449914, ECO:0000269|PubMed:24915079, ECO:0000269|PubMed:26272610, ECO:0000269|PubMed:26349033, ECO:0000269|PubMed:28282025, ECO:0000269|PubMed:7885480, ECO:0007744|PDB:1IBR, ECO:0007744|PDB:1K5G, ECO:0007744|PDB:1RRP, ECO:0007744|PDB:3CH5, ECO:0007744|PDB:3GJ0, ECO:0007744|PDB:3GJ3, ECO:0007744|PDB:3GJ4, ECO:0007744|PDB:3GJ5, ECO:0007744|PDB:3GJ6, ECO:0007744|PDB:3GJ7, ECO:0007744|PDB:3GJ8, ECO:0007744|PDB:3GJX, ECO:0007744|PDB:3NBY, ECO:0007744|PDB:3NBZ, ECO:0007744|PDB:3NC0, ECO:0007744|PDB:3NC1, ECO:0007744|PDB:3ZJY, ECO:0007744|PDB:4C0Q, ECO:0007744|PDB:4OL0, ECO:0007744|PDB:5CIQ, ECO:0007744|PDB:5CIT, ECO:0007744|PDB:5CIW, ECO:0007744|PDB:5CLQ, ECO:0007744|PDB:5DH9, ECO:0007744|PDB:5DHA, ECO:0007744|PDB:5DHF, ECO:0007744|PDB:5DI9, ECO:0007744|PDB:5DIF, ECO:0007744|PDB:5DIS, ECO:0007744|PDB:5DLQ, ECO:0007744|PDB:5UWH, ECO:0007744|PDB:5UWI, ECO:0007744|PDB:5UWJ, ECO:0007744|PDB:5UWO, ECO:0007744|PDB:5UWP, ECO:0007744|PDB:5UWQ, ECO:0007744|PDB:5UWR, ECO:0007744|PDB:5UWS, ECO:0007744|PDB:5UWT, ECO:0007744|PDB:5UWU, ECO:0007744|PDB:5UWW

Chain	Residue	Details
C	ASP18
F	ASP18
B	TRP276
E	SER105
E	LYS144
E	TRP276

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10078529, ECO:0000269|PubMed:10353245, ECO:0000269|PubMed:10367892, ECO:0000269|PubMed:11832950, ECO:0000269|PubMed:19389996, ECO:0000269|PubMed:24449914, ECO:0000269|PubMed:24915079, ECO:0000269|PubMed:26272610, ECO:0000269|PubMed:26349033, ECO:0000269|PubMed:28282025, ECO:0000269|PubMed:7885480, ECO:0007744|PDB:1IBR, ECO:0007744|PDB:1K5D, ECO:0007744|PDB:1QBK, ECO:0007744|PDB:1RRP, ECO:0007744|PDB:3GJX, ECO:0007744|PDB:3NBY, ECO:0007744|PDB:3NBZ, ECO:0007744|PDB:3NC0, ECO:0007744|PDB:3NC1, ECO:0007744|PDB:3ZJY, ECO:0007744|PDB:4C0Q, ECO:0007744|PDB:4OL0, ECO:0007744|PDB:5CLL, ECO:0007744|PDB:5CLQ, ECO:0007744|PDB:5DH9, ECO:0007744|PDB:5DHA, ECO:0007744|PDB:5DHF, ECO:0007744|PDB:5DI9, ECO:0007744|PDB:5DIF, ECO:0007744|PDB:5DIS, ECO:0007744|PDB:5DLQ, ECO:0007744|PDB:5JLJ, ECO:0007744|PDB:5UWH, ECO:0007744|PDB:5UWI, ECO:0007744|PDB:5UWJ, ECO:0007744|PDB:5UWO, ECO:0007744|PDB:5UWP, ECO:0007744|PDB:5UWQ, ECO:0007744|PDB:5UWR, ECO:0007744|PDB:5UWS, ECO:0007744|PDB:5UWT, ECO:0007744|PDB:5UWU, ECO:0007744|PDB:5UWW

Chain	Residue	Details
C	GLU36
F	GLU36

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10078529, ECO:0000269|PubMed:10353245, ECO:0000269|PubMed:10367892, ECO:0000269|PubMed:11832950, ECO:0000269|PubMed:18611384, ECO:0000269|PubMed:19389996, ECO:0000269|PubMed:19505478, ECO:0000269|PubMed:24449914, ECO:0000269|PubMed:24915079, ECO:0000269|PubMed:26272610, ECO:0000269|PubMed:26349033, ECO:0000269|PubMed:28282025, ECO:0000269|PubMed:7885480, ECO:0007744|PDB:1IBR, ECO:0007744|PDB:1K5D, ECO:0007744|PDB:1QBK, ECO:0007744|PDB:1RRP, ECO:0007744|PDB:3GJX, ECO:0007744|PDB:3NBY, ECO:0007744|PDB:3NBZ, ECO:0007744|PDB:3NC0, ECO:0007744|PDB:3NC1, ECO:0007744|PDB:3ZJY, ECO:0007744|PDB:4C0Q, ECO:0007744|PDB:4OL0, ECO:0007744|PDB:5DH9, ECO:0007744|PDB:5DHA, ECO:0007744|PDB:5DHF, ECO:0007744|PDB:5DI9, ECO:0007744|PDB:5DIF, ECO:0007744|PDB:5DIS, ECO:0007744|PDB:5DLQ, ECO:0007744|PDB:5JLJ, ECO:0007744|PDB:5UWH, ECO:0007744|PDB:5UWI, ECO:0007744|PDB:5UWJ, ECO:0007744|PDB:5UWO, ECO:0007744|PDB:5UWP, ECO:0007744|PDB:5UWQ, ECO:0007744|PDB:5UWR, ECO:0007744|PDB:5UWS, ECO:0007744|PDB:5UWT, ECO:0007744|PDB:5UWU, ECO:0007744|PDB:5UWW

Chain	Residue	Details
C	GLY68
F	GLY68

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10078529, ECO:0000269|PubMed:10353245, ECO:0000269|PubMed:10367892, ECO:0000269|PubMed:11832950, ECO:0000269|PubMed:18611384, ECO:0000269|PubMed:19389996, ECO:0000269|PubMed:19505478, ECO:0000269|PubMed:24449914, ECO:0000269|PubMed:24915079, ECO:0000269|PubMed:26272610, ECO:0000269|PubMed:26349033, ECO:0000269|PubMed:28282025, ECO:0000269|PubMed:7885480, ECO:0007744|PDB:1IBR, ECO:0007744|PDB:1K5D, ECO:0007744|PDB:1QBK, ECO:0007744|PDB:1RRP, ECO:0007744|PDB:3CH5, ECO:0007744|PDB:3GJ0, ECO:0007744|PDB:3GJ3, ECO:0007744|PDB:3GJ4, ECO:0007744|PDB:3GJ5, ECO:0007744|PDB:3GJ6, ECO:0007744|PDB:3GJ7, ECO:0007744|PDB:3GJ8, ECO:0007744|PDB:3GJX, ECO:0007744|PDB:3NBY, ECO:0007744|PDB:3NBZ, ECO:0007744|PDB:3NC0, ECO:0007744|PDB:3NC1, ECO:0007744|PDB:3ZJY, ECO:0007744|PDB:4C0Q, ECO:0007744|PDB:4OL0, ECO:0007744|PDB:5CIQ, ECO:0007744|PDB:5CIT, ECO:0007744|PDB:5CIW, ECO:0007744|PDB:5CJ2, ECO:0007744|PDB:5CLL, ECO:0007744|PDB:5DH9, ECO:0007744|PDB:5DHA, ECO:0007744|PDB:5DHF, ECO:0007744|PDB:5DI9, ECO:0007744|PDB:5DIF, ECO:0007744|PDB:5DIS, ECO:0007744|PDB:5DLQ, ECO:0007744|PDB:5JLJ, ECO:0007744|PDB:5UWH, ECO:0007744|PDB:5UWI, ECO:0007744|PDB:5UWJ, ECO:0007744|PDB:5UWO, ECO:0007744|PDB:5UWP, ECO:0007744|PDB:5UWQ, ECO:0007744|PDB:5UWR, ECO:0007744|PDB:5UWS, ECO:0007744|PDB:5UWT, ECO:0007744|PDB:5UWU, ECO:0007744|PDB:5UWW

Chain	Residue	Details
C	ASN122
F	ASN122

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:10078529, ECO:0000269|PubMed:10353245, ECO:0000269|PubMed:10367892, ECO:0000269|PubMed:11832950, ECO:0000269|PubMed:18611384, ECO:0000269|PubMed:19389996, ECO:0000269|PubMed:19505478, ECO:0000269|PubMed:24449914, ECO:0000269|PubMed:24915079, ECO:0000269|PubMed:26272610, ECO:0000269|PubMed:26349033, ECO:0000269|PubMed:28282025, ECO:0000269|PubMed:7885480, ECO:0007744|PDB:1K5G, ECO:0007744|PDB:3CH5, ECO:0007744|PDB:3GJ0, ECO:0007744|PDB:3GJ3, ECO:0007744|PDB:3GJ4, ECO:0007744|PDB:3GJ5, ECO:0007744|PDB:3GJ6, ECO:0007744|PDB:3GJ7, ECO:0007744|PDB:3GJ8, ECO:0007744|PDB:3GJX, ECO:0007744|PDB:3NBY, ECO:0007744|PDB:3NBZ, ECO:0007744|PDB:3NC0, ECO:0007744|PDB:3NC1, ECO:0007744|PDB:3ZJY, ECO:0007744|PDB:4C0Q, ECO:0007744|PDB:4OL0, ECO:0007744|PDB:5CIQ, ECO:0007744|PDB:5CIT, ECO:0007744|PDB:5CIW, ECO:0007744|PDB:5CJ2, ECO:0007744|PDB:5CLL, ECO:0007744|PDB:5CLQ, ECO:0007744|PDB:5DH9, ECO:0007744|PDB:5DHA, ECO:0007744|PDB:5DHF, ECO:0007744|PDB:5DI9, ECO:0007744|PDB:5DIF, ECO:0007744|PDB:5DIS, ECO:0007744|PDB:5DLQ, ECO:0007744|PDB:5JLJ, ECO:0007744|PDB:5UWH, ECO:0007744|PDB:5UWI, ECO:0007744|PDB:5UWJ, ECO:0007744|PDB:5UWO, ECO:0007744|PDB:5UWP, ECO:0007744|PDB:5UWQ, ECO:0007744|PDB:5UWR, ECO:0007744|PDB:5UWS, ECO:0007744|PDB:5UWT, ECO:0007744|PDB:5UWU, ECO:0007744|PDB:5UWW

Chain	Residue	Details
C	SER150
F	SER150

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Essential for GTP hydrolysis => ECO:0000269|PubMed:18591255, ECO:0000269|PubMed:26272610, ECO:0000269|PubMed:8636225

Chain	Residue	Details
C	LEU69
F	LEU69

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
C	THR24
F	THR24

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:31075303

Chain	Residue	Details
C	LYS37
F	LYS37

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
C	LYS60
C	LYS99
F	LYS60
F	LYS99

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
C	LYS71
F	LYS71

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:29040603

Chain	Residue	Details
C	LYS134
F	LYS134

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P62827

Chain	Residue	Details
C	LYS159
F	LYS159

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site_id	SWS_FT_FI13
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate

Chain	Residue	Details
C	LYS71
F	LYS71

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site_id	SWS_FT_FI14
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
C	LYS152
F	LYS152

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