3NC0
Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.262, 225.895, 163.984 |
| Unit cell angles | 90.00, 100.75, 90.00 |
Refinement procedure
| Resolution | 39.046 - 2.900 |
| R-factor | 0.2448 |
| Rwork | 0.242 |
| R-free | 0.29470 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3gjx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.747 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.143 |
| Rmeas | 0.162 |
| Total number of observations | 465206 |
| Number of reflections | 110353 |
| <I/σ(I)> | 8.97 |
| Completeness [%] | 95.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1 M Tris/HCl, 16% (w/v) PEG 1000, 2 mM phenol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
