3MVJ

Human cyclic AMP-dependent protein kinase PKA inhibitor complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001669	cellular_component	acrosomal vesicle
A	0001707	biological_process	mesoderm formation
A	0001843	biological_process	neural tube closure
A	0002027	biological_process	regulation of heart rate
A	0003091	biological_process	renal water homeostasis
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004691	molecular_function	cAMP-dependent protein kinase activity
A	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005930	cellular_component	axoneme
A	0005952	cellular_component	cAMP-dependent protein kinase complex
A	0006397	biological_process	mRNA processing
A	0006468	biological_process	protein phosphorylation
A	0006611	biological_process	protein export from nucleus
A	0007165	biological_process	signal transduction
A	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
A	0016241	biological_process	regulation of macroautophagy
A	0016607	cellular_component	nuclear speck
A	0018105	biological_process	peptidyl-serine phosphorylation
A	0019221	biological_process	cytokine-mediated signaling pathway
A	0019901	molecular_function	protein kinase binding
A	0019904	molecular_function	protein domain specific binding
A	0030145	molecular_function	manganese ion binding
A	0031410	cellular_component	cytoplasmic vesicle
A	0031514	cellular_component	motile cilium
A	0031594	cellular_component	neuromuscular junction
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0032024	biological_process	positive regulation of insulin secretion
A	0032703	biological_process	negative regulation of interleukin-2 production
A	0034237	molecular_function	protein kinase A regulatory subunit binding
A	0034380	biological_process	high-density lipoprotein particle assembly
A	0034605	biological_process	cellular response to heat
A	0034704	cellular_component	calcium channel complex
A	0035694	biological_process	mitochondrial protein catabolic process
A	0036126	cellular_component	sperm flagellum
A	0044853	cellular_component	plasma membrane raft
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0045667	biological_process	regulation of osteoblast differentiation
A	0045722	biological_process	positive regulation of gluconeogenesis
A	0045879	biological_process	negative regulation of smoothened signaling pathway
A	0046827	biological_process	positive regulation of protein export from nucleus
A	0048240	biological_process	sperm capacitation
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050804	biological_process	modulation of chemical synaptic transmission
A	0050850	biological_process	positive regulation of calcium-mediated signaling
A	0051726	biological_process	regulation of cell cycle
A	0055117	biological_process	regulation of cardiac muscle contraction
A	0061136	biological_process	regulation of proteasomal protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	0070417	biological_process	cellular response to cold
A	0070613	biological_process	regulation of protein processing
A	0071333	biological_process	cellular response to glucose stimulus
A	0071374	biological_process	cellular response to parathyroid hormone stimulus
A	0071377	biological_process	cellular response to glucagon stimulus
A	0071872	biological_process	cellular response to epinephrine stimulus
A	0086064	biological_process	cell communication by electrical coupling involved in cardiac conduction
A	0097546	cellular_component	ciliary base
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099103	molecular_function	channel activator activity
A	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
A	0106310	molecular_function	protein serine kinase activity
A	1903779	biological_process	regulation of cardiac conduction
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
A	1990044	biological_process	protein localization to lipid droplet
A	2000810	biological_process	regulation of bicellular tight junction assembly
B	0000287	molecular_function	magnesium ion binding
B	0001669	cellular_component	acrosomal vesicle
B	0001707	biological_process	mesoderm formation
B	0001843	biological_process	neural tube closure
B	0002027	biological_process	regulation of heart rate
B	0003091	biological_process	renal water homeostasis
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004691	molecular_function	cAMP-dependent protein kinase activity
B	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005813	cellular_component	centrosome
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005930	cellular_component	axoneme
B	0005952	cellular_component	cAMP-dependent protein kinase complex
B	0006397	biological_process	mRNA processing
B	0006468	biological_process	protein phosphorylation
B	0006611	biological_process	protein export from nucleus
B	0007165	biological_process	signal transduction
B	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
B	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
B	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
B	0016241	biological_process	regulation of macroautophagy
B	0016607	cellular_component	nuclear speck
B	0018105	biological_process	peptidyl-serine phosphorylation
B	0019221	biological_process	cytokine-mediated signaling pathway
B	0019901	molecular_function	protein kinase binding
B	0019904	molecular_function	protein domain specific binding
B	0030145	molecular_function	manganese ion binding
B	0031410	cellular_component	cytoplasmic vesicle
B	0031514	cellular_component	motile cilium
B	0031594	cellular_component	neuromuscular junction
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032024	biological_process	positive regulation of insulin secretion
B	0032703	biological_process	negative regulation of interleukin-2 production
B	0034237	molecular_function	protein kinase A regulatory subunit binding
B	0034380	biological_process	high-density lipoprotein particle assembly
B	0034605	biological_process	cellular response to heat
B	0034704	cellular_component	calcium channel complex
B	0035694	biological_process	mitochondrial protein catabolic process
B	0036126	cellular_component	sperm flagellum
B	0044853	cellular_component	plasma membrane raft
B	0045542	biological_process	positive regulation of cholesterol biosynthetic process
B	0045667	biological_process	regulation of osteoblast differentiation
B	0045722	biological_process	positive regulation of gluconeogenesis
B	0045879	biological_process	negative regulation of smoothened signaling pathway
B	0046827	biological_process	positive regulation of protein export from nucleus
B	0048240	biological_process	sperm capacitation
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050804	biological_process	modulation of chemical synaptic transmission
B	0050850	biological_process	positive regulation of calcium-mediated signaling
B	0051726	biological_process	regulation of cell cycle
B	0055117	biological_process	regulation of cardiac muscle contraction
B	0061136	biological_process	regulation of proteasomal protein catabolic process
B	0070062	cellular_component	extracellular exosome
B	0070417	biological_process	cellular response to cold
B	0070613	biological_process	regulation of protein processing
B	0071333	biological_process	cellular response to glucose stimulus
B	0071374	biological_process	cellular response to parathyroid hormone stimulus
B	0071377	biological_process	cellular response to glucagon stimulus
B	0071872	biological_process	cellular response to epinephrine stimulus
B	0086064	biological_process	cell communication by electrical coupling involved in cardiac conduction
B	0097546	cellular_component	ciliary base
B	0098794	cellular_component	postsynapse
B	0098978	cellular_component	glutamatergic synapse
B	0099103	molecular_function	channel activator activity
B	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
B	0106310	molecular_function	protein serine kinase activity
B	1903779	biological_process	regulation of cardiac conduction
B	1904262	biological_process	negative regulation of TORC1 signaling
B	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
B	1990044	biological_process	protein localization to lipid droplet
B	2000810	biological_process	regulation of bicellular tight junction assembly
E	0000287	molecular_function	magnesium ion binding
E	0001669	cellular_component	acrosomal vesicle
E	0001707	biological_process	mesoderm formation
E	0001843	biological_process	neural tube closure
E	0002027	biological_process	regulation of heart rate
E	0003091	biological_process	renal water homeostasis
E	0004672	molecular_function	protein kinase activity
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0004691	molecular_function	cAMP-dependent protein kinase activity
E	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005930	cellular_component	axoneme
E	0005952	cellular_component	cAMP-dependent protein kinase complex
E	0006397	biological_process	mRNA processing
E	0006468	biological_process	protein phosphorylation
E	0006611	biological_process	protein export from nucleus
E	0007165	biological_process	signal transduction
E	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
E	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
E	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
E	0016241	biological_process	regulation of macroautophagy
E	0016607	cellular_component	nuclear speck
E	0018105	biological_process	peptidyl-serine phosphorylation
E	0019221	biological_process	cytokine-mediated signaling pathway
E	0019901	molecular_function	protein kinase binding
E	0019904	molecular_function	protein domain specific binding
E	0030145	molecular_function	manganese ion binding
E	0031410	cellular_component	cytoplasmic vesicle
E	0031514	cellular_component	motile cilium
E	0031594	cellular_component	neuromuscular junction
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0032024	biological_process	positive regulation of insulin secretion
E	0032703	biological_process	negative regulation of interleukin-2 production
E	0034237	molecular_function	protein kinase A regulatory subunit binding
E	0034380	biological_process	high-density lipoprotein particle assembly
E	0034605	biological_process	cellular response to heat
E	0034704	cellular_component	calcium channel complex
E	0035694	biological_process	mitochondrial protein catabolic process
E	0036126	cellular_component	sperm flagellum
E	0044853	cellular_component	plasma membrane raft
E	0045542	biological_process	positive regulation of cholesterol biosynthetic process
E	0045667	biological_process	regulation of osteoblast differentiation
E	0045722	biological_process	positive regulation of gluconeogenesis
E	0045879	biological_process	negative regulation of smoothened signaling pathway
E	0046827	biological_process	positive regulation of protein export from nucleus
E	0048240	biological_process	sperm capacitation
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050804	biological_process	modulation of chemical synaptic transmission
E	0050850	biological_process	positive regulation of calcium-mediated signaling
E	0051726	biological_process	regulation of cell cycle
E	0055117	biological_process	regulation of cardiac muscle contraction
E	0061136	biological_process	regulation of proteasomal protein catabolic process
E	0070062	cellular_component	extracellular exosome
E	0070417	biological_process	cellular response to cold
E	0070613	biological_process	regulation of protein processing
E	0071333	biological_process	cellular response to glucose stimulus
E	0071374	biological_process	cellular response to parathyroid hormone stimulus
E	0071377	biological_process	cellular response to glucagon stimulus
E	0071872	biological_process	cellular response to epinephrine stimulus
E	0086064	biological_process	cell communication by electrical coupling involved in cardiac conduction
E	0097546	cellular_component	ciliary base
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0099103	molecular_function	channel activator activity
E	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
E	0106310	molecular_function	protein serine kinase activity
E	1903779	biological_process	regulation of cardiac conduction
E	1904262	biological_process	negative regulation of TORC1 signaling
E	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
E	1990044	biological_process	protein localization to lipid droplet
E	2000810	biological_process	regulation of bicellular tight junction assembly
I	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
I	0006469	biological_process	negative regulation of protein kinase activity
J	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
J	0006469	biological_process	negative regulation of protein kinase activity
K	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
K	0006469	biological_process	negative regulation of protein kinase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE XFE A 351

Chain	Residue
A	LEU49
A	HOH407
J	ARG18
A	ALA70
A	GLU121
A	VAL123
A	GLU127
A	GLU170
A	LEU173
A	THR183
A	PHE327

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE XFE E 351

Chain	Residue
E	LEU49
E	GLY50
E	ALA70
E	GLU121
E	VAL123
E	GLU127
E	LEU173
E	THR183
E	PHE327

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhketgnh..........YAMK

Chain	Residue	Details
A	LEU49-LYS72

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI

Chain	Residue	Details
A	LEU162-ILE174

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	9
Details	SITE: Important for inhibition => ECO:0000250

Chain	Residue	Details
I	ARG15
I	ARG18
I	ARG19
J	ARG15
J	ARG18
J	ARG19
K	ARG15
K	ARG18
K	ARG19

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	LEU49
B	LEU49
E	LEU49

---

site_id	SWS_FT_FI3
Number of Residues	9
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159

Chain	Residue	Details
A	LYS72
A	GLU121
A	LYS168
B	LYS72
B	GLU121
B	LYS168
E	LYS72
E	GLU121
E	LYS168

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: Deamidated asparagine => ECO:0000250|UniProtKB:P05132

Chain	Residue	Details
A	ASN2
B	ASN2
E	ASN2

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: Phosphoserine; by autocatalysis => ECO:0000250|UniProtKB:P05132

Chain	Residue	Details
A	SER10
B	SER10
E	SER10

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18691976

Chain	Residue	Details
A	THR48
B	THR48
E	THR48

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P05132

Chain	Residue	Details
A	SER139
B	SER139
E	SER139

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:12372837

Chain	Residue	Details
A	THR195
B	THR195
E	THR195

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269|PubMed:12372837, ECO:0000269|PubMed:16765046, ECO:0000269|PubMed:20137943, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:20732331, ECO:0000269|PubMed:21774789, ECO:0000269|Ref.43

Chain	Residue	Details
A	TPO197
B	TPO197
E	TPO197

---

site_id	SWS_FT_FI10
Number of Residues	3
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P05132

Chain	Residue	Details
A	TYR330
B	TYR330
E	TYR330

---

site_id	SWS_FT_FI11
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:16765046, ECO:0000269|PubMed:20137943, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:20732331, ECO:0000269|PubMed:21774789, ECO:0000269|Ref.43, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SEP338
B	SEP338
E	SEP338

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	LIPID: N-myristoyl glycine => ECO:0000269|PubMed:25255805, ECO:0000269|PubMed:25807930

Chain	Residue	Details
A	GLY1
B	GLY1
E	GLY1

---