Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3MPU

Crystal structure of the C47A/A241C disulfide-linked E. coli Aspartate Transcarbamoylase holoenzyme

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004070	molecular_function	aspartate carbamoyltransferase activity
A	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006520	biological_process	amino acid metabolic process
A	0006541	biological_process	glutamine metabolic process
A	0009347	cellular_component	aspartate carbamoyltransferase complex
A	0016597	molecular_function	amino acid binding
A	0016740	molecular_function	transferase activity
A	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
A	0042802	molecular_function	identical protein binding
A	0044205	biological_process	'de novo' UMP biosynthetic process
A	0070207	biological_process	protein homotrimerization
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
B	0006221	biological_process	pyrimidine nucleotide biosynthetic process
B	0008270	molecular_function	zinc ion binding
B	0009347	cellular_component	aspartate carbamoyltransferase complex
B	0046872	molecular_function	metal ion binding
C	0004070	molecular_function	aspartate carbamoyltransferase activity
C	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
C	0006221	biological_process	pyrimidine nucleotide biosynthetic process
C	0006520	biological_process	amino acid metabolic process
C	0006541	biological_process	glutamine metabolic process
C	0009347	cellular_component	aspartate carbamoyltransferase complex
C	0016597	molecular_function	amino acid binding
C	0016740	molecular_function	transferase activity
C	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
C	0042802	molecular_function	identical protein binding
C	0044205	biological_process	'de novo' UMP biosynthetic process
C	0070207	biological_process	protein homotrimerization
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
D	0006221	biological_process	pyrimidine nucleotide biosynthetic process
D	0008270	molecular_function	zinc ion binding
D	0009347	cellular_component	aspartate carbamoyltransferase complex
D	0046872	molecular_function	metal ion binding
E	0004070	molecular_function	aspartate carbamoyltransferase activity
E	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
E	0006221	biological_process	pyrimidine nucleotide biosynthetic process
E	0006520	biological_process	amino acid metabolic process
E	0006541	biological_process	glutamine metabolic process
E	0009347	cellular_component	aspartate carbamoyltransferase complex
E	0016597	molecular_function	amino acid binding
E	0016740	molecular_function	transferase activity
E	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
E	0042802	molecular_function	identical protein binding
E	0044205	biological_process	'de novo' UMP biosynthetic process
E	0070207	biological_process	protein homotrimerization
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
F	0006221	biological_process	pyrimidine nucleotide biosynthetic process
F	0008270	molecular_function	zinc ion binding
F	0009347	cellular_component	aspartate carbamoyltransferase complex
F	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 A 311

Chain	Residue
A	SER52
A	HOH514
A	THR53
A	ARG54
A	THR55
A	SER80
A	LYS84
A	ARG105
A	LEU267
A	PO4312

site_id	AC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PO4 A 312

Chain	Residue
A	THR55
A	LYS84
A	ARG105
A	HIS134
A	ARG167
A	THR168
A	LEU267
A	PO4311
A	HOH514
A	HOH563
A	HOH571

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 154

Chain	Residue
B	CYS109
B	CYS114
B	CYS138
B	CYS141

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 C 311

Chain	Residue
C	SER52
C	THR53
C	ARG54
C	THR55
C	SER80
C	LYS84
C	ARG105
C	LEU267
C	PO4312
C	HOH680

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 C 312

Chain	Residue
C	THR55
C	LYS84
C	ARG105
C	HIS134
C	ARG167
C	THR168
C	LEU267
C	PO4311
C	HOH680
C	HOH729

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 154

Chain	Residue
D	CYS109
D	CYS114
D	CYS138
D	CYS141

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 E 311

Chain	Residue
E	SER52
E	THR53
E	ARG54
E	THR55
E	SER80
E	ARG105
E	LEU267
E	PO4312
E	HOH709

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 E 312

Chain	Residue
E	THR55
E	LYS84
E	ARG105
E	HIS134
E	ARG167
E	THR168
E	LEU267
E	PO4311
E	HOH319
E	HOH709

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN F 154

Chain	Residue
F	CYS109
F	CYS114
F	CYS138
F	CYS141

Functional Information from PROSITE/UniProt

site_id	PS00097
Number of Residues	8
Details	CARBAMOYLTRANSFERASE Aspartate and ornithine carbamoyltransferases signature. FfEaSTRT

Chain	Residue	Details
A	PHE48-THR55

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	BINDING:

Chain	Residue	Details
B	CYS109
F	CYS114
F	CYS138
F	CYS141
C	LEU267
C	PRO268
E	ARG54
E	THR55
E	ARG105
E	HIS134
E	GLN137
B	CYS114
E	LEU267
E	PRO268
B	CYS138
B	CYS141
D	CYS109
D	CYS114
D	CYS138
D	CYS141
F	CYS109

site_id	SWS_FT_FI2
Number of Residues	9
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00001, ECO:0000305\|PubMed:3380787

Chain	Residue	Details
A	LYS84
A	ARG167
A	ARG229
C	LYS84
C	ARG167
C	ARG229
E	LYS84
E	ARG167
E	ARG229

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
A	ARG54	electrostatic stabiliser
A	THR55	electrostatic stabiliser, increase electrophilicity
A	LYS84	proton shuttle (general acid/base)
A	ARG105	electrostatic stabiliser, increase electrophilicity
A	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA2
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
C	ARG54	electrostatic stabiliser
C	THR55	electrostatic stabiliser, increase electrophilicity
C	LYS84	proton shuttle (general acid/base)
C	ARG105	electrostatic stabiliser, increase electrophilicity
C	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA3
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
E	ARG54	electrostatic stabiliser
E	THR55	electrostatic stabiliser, increase electrophilicity
E	LYS84	proton shuttle (general acid/base)
E	ARG105	electrostatic stabiliser, increase electrophilicity
E	HIS134	electrostatic stabiliser, increase electrophilicity

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)