3L3T

Human mesotrypsin complexed with amyloid precursor protein inhibitor variant (APPIR15K)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0006508	biological_process	proteolysis
B	0004252	molecular_function	serine-type endopeptidase activity
B	0006508	biological_process	proteolysis
C	0004252	molecular_function	serine-type endopeptidase activity
C	0006508	biological_process	proteolysis
D	0004252	molecular_function	serine-type endopeptidase activity
D	0006508	biological_process	proteolysis
E	0004867	molecular_function	serine-type endopeptidase inhibitor activity
F	0004867	molecular_function	serine-type endopeptidase inhibitor activity
G	0004867	molecular_function	serine-type endopeptidase inhibitor activity
H	0004867	molecular_function	serine-type endopeptidase inhibitor activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE FMT A 1

Chain	Residue
A	LEU163
A	HOH300

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site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 2

Chain	Residue
A	ARG66
A	ILE73
A	HOH269
A	HOH341

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE FMT B 3

Chain	Residue
B	PRO129
B	LEU163

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 4

Chain	Residue
A	TYR234
A	VAL235
A	ASP236
A	TRP237
A	ASN233

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 247

Chain	Residue
A	ALA221
A	TRP221
A	LYS222
A	HOH327

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 248

Chain	Residue
A	SER119
A	THR120
A	HOH205
A	HOH301
C	SER122
C	GLY203

---

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE FMT C 247

Chain	Residue
C	PRO129
C	LYS230

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE FMT B 10

Chain	Residue
B	SER171
B	ASN223

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FMT B 11

Chain	Residue
B	HIS91
B	LYS93
C	GLY174

---

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE FMT B 12

Chain	Residue
A	THR164
B	THR164

---

site_id	BC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE FMT A 13

Chain	Residue
A	GLY219

---

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA B 1

Chain	Residue
B	GLU70
B	ASN72
B	VAL75
B	GLU77
B	GLU80

---

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 250

Chain	Residue
A	GLU70
A	ASN72
A	VAL75
A	GLU77
A	GLU80
A	HOH269

---

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA C 3

Chain	Residue
C	GLU70
C	ASN72
C	VAL75
C	GLU77
C	GLU80
C	HOH289

---

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA D 4

Chain	Residue
D	GLU70
D	ASN72
D	VAL75
D	GLU80
D	HOH267

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VSAAHC

Chain	Residue	Details
A	VAL53-CYS58

---

site_id	PS00280
Number of Residues	19
Details	BPTI_KUNITZ_1 Pancreatic trypsin inhibitor (Kunitz) family signature. FfyGGCggnrnnFdteeyC

Chain	Residue	Details
E	PHE33-CYS51

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Charge relay system => ECO:0000255|PROSITE-ProRule:PRU00274, ECO:0000305|PubMed:27810896

Chain	Residue	Details
A	HIS57
D	HIS57
D	ASP102
D	ALA195
A	ASP102
A	ALA195
B	HIS57
B	ASP102
B	ALA195
C	HIS57
C	ASP102
C	ALA195

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U30, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT, ECO:0007744|PDB:5TP0

Chain	Residue	Details
A	GLU70
D	GLU70
D	ASN72
D	VAL75
A	ASN72
A	VAL75
B	GLU70
B	ASN72
B	VAL75
C	GLU70
C	ASN72
C	VAL75

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U30, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT

Chain	Residue	Details
A	GLU77
B	GLU77
C	GLU77
D	GLU77

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT, ECO:0007744|PDB:5TP0

Chain	Residue	Details
A	GLU80
B	GLU80
C	GLU80
D	GLU80

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Required for specificity => ECO:0000250

Chain	Residue	Details
A	ASP189
B	ASP189
C	ASP189
D	ASP189

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Sulfotyrosine => ECO:0000250

Chain	Residue	Details
A	TYR151
B	TYR151
C	TYR151
D	TYR151

---