Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3JBL

Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0002218	biological_process	activation of innate immune response
A	0002376	biological_process	immune system process
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006915	biological_process	apoptotic process
A	0006954	biological_process	inflammatory response
A	0010954	biological_process	positive regulation of protein processing
A	0016045	biological_process	detection of bacterium
A	0032731	biological_process	positive regulation of interleukin-1 beta production
A	0042742	biological_process	defense response to bacterium
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0042981	biological_process	regulation of apoptotic process
A	0043065	biological_process	positive regulation of apoptotic process
A	0045087	biological_process	innate immune response
A	0051260	biological_process	protein homooligomerization
A	0061133	molecular_function	endopeptidase activator activity
A	0061702	cellular_component	canonical inflammasome complex
A	0070269	biological_process	pyroptotic inflammatory response
A	0072557	cellular_component	IPAF inflammasome complex
A	0089720	molecular_function	caspase binding
B	0000166	molecular_function	nucleotide binding
B	0002218	biological_process	activation of innate immune response
B	0002376	biological_process	immune system process
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006915	biological_process	apoptotic process
B	0006954	biological_process	inflammatory response
B	0010954	biological_process	positive regulation of protein processing
B	0016045	biological_process	detection of bacterium
B	0032731	biological_process	positive regulation of interleukin-1 beta production
B	0042742	biological_process	defense response to bacterium
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0042981	biological_process	regulation of apoptotic process
B	0043065	biological_process	positive regulation of apoptotic process
B	0045087	biological_process	innate immune response
B	0051260	biological_process	protein homooligomerization
B	0061133	molecular_function	endopeptidase activator activity
B	0061702	cellular_component	canonical inflammasome complex
B	0070269	biological_process	pyroptotic inflammatory response
B	0072557	cellular_component	IPAF inflammasome complex
B	0089720	molecular_function	caspase binding
C	0000166	molecular_function	nucleotide binding
C	0002218	biological_process	activation of innate immune response
C	0002376	biological_process	immune system process
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006915	biological_process	apoptotic process
C	0006954	biological_process	inflammatory response
C	0010954	biological_process	positive regulation of protein processing
C	0016045	biological_process	detection of bacterium
C	0032731	biological_process	positive regulation of interleukin-1 beta production
C	0042742	biological_process	defense response to bacterium
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0042981	biological_process	regulation of apoptotic process
C	0043065	biological_process	positive regulation of apoptotic process
C	0045087	biological_process	innate immune response
C	0051260	biological_process	protein homooligomerization
C	0061133	molecular_function	endopeptidase activator activity
C	0061702	cellular_component	canonical inflammasome complex
C	0070269	biological_process	pyroptotic inflammatory response
C	0072557	cellular_component	IPAF inflammasome complex
C	0089720	molecular_function	caspase binding
D	0000166	molecular_function	nucleotide binding
D	0002218	biological_process	activation of innate immune response
D	0002376	biological_process	immune system process
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006915	biological_process	apoptotic process
D	0006954	biological_process	inflammatory response
D	0010954	biological_process	positive regulation of protein processing
D	0016045	biological_process	detection of bacterium
D	0032731	biological_process	positive regulation of interleukin-1 beta production
D	0042742	biological_process	defense response to bacterium
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0042981	biological_process	regulation of apoptotic process
D	0043065	biological_process	positive regulation of apoptotic process
D	0045087	biological_process	innate immune response
D	0051260	biological_process	protein homooligomerization
D	0061133	molecular_function	endopeptidase activator activity
D	0061702	cellular_component	canonical inflammasome complex
D	0070269	biological_process	pyroptotic inflammatory response
D	0072557	cellular_component	IPAF inflammasome complex
D	0089720	molecular_function	caspase binding
E	0000166	molecular_function	nucleotide binding
E	0002218	biological_process	activation of innate immune response
E	0002376	biological_process	immune system process
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006915	biological_process	apoptotic process
E	0006954	biological_process	inflammatory response
E	0010954	biological_process	positive regulation of protein processing
E	0016045	biological_process	detection of bacterium
E	0032731	biological_process	positive regulation of interleukin-1 beta production
E	0042742	biological_process	defense response to bacterium
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0042981	biological_process	regulation of apoptotic process
E	0043065	biological_process	positive regulation of apoptotic process
E	0045087	biological_process	innate immune response
E	0051260	biological_process	protein homooligomerization
E	0061133	molecular_function	endopeptidase activator activity
E	0061702	cellular_component	canonical inflammasome complex
E	0070269	biological_process	pyroptotic inflammatory response
E	0072557	cellular_component	IPAF inflammasome complex
E	0089720	molecular_function	caspase binding
F	0000166	molecular_function	nucleotide binding
F	0002218	biological_process	activation of innate immune response
F	0002376	biological_process	immune system process
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006915	biological_process	apoptotic process
F	0006954	biological_process	inflammatory response
F	0010954	biological_process	positive regulation of protein processing
F	0016045	biological_process	detection of bacterium
F	0032731	biological_process	positive regulation of interleukin-1 beta production
F	0042742	biological_process	defense response to bacterium
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0042981	biological_process	regulation of apoptotic process
F	0043065	biological_process	positive regulation of apoptotic process
F	0045087	biological_process	innate immune response
F	0051260	biological_process	protein homooligomerization
F	0061133	molecular_function	endopeptidase activator activity
F	0061702	cellular_component	canonical inflammasome complex
F	0070269	biological_process	pyroptotic inflammatory response
F	0072557	cellular_component	IPAF inflammasome complex
F	0089720	molecular_function	caspase binding
G	0000166	molecular_function	nucleotide binding
G	0002218	biological_process	activation of innate immune response
G	0002376	biological_process	immune system process
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0006915	biological_process	apoptotic process
G	0006954	biological_process	inflammatory response
G	0010954	biological_process	positive regulation of protein processing
G	0016045	biological_process	detection of bacterium
G	0032731	biological_process	positive regulation of interleukin-1 beta production
G	0042742	biological_process	defense response to bacterium
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0042981	biological_process	regulation of apoptotic process
G	0043065	biological_process	positive regulation of apoptotic process
G	0045087	biological_process	innate immune response
G	0051260	biological_process	protein homooligomerization
G	0061133	molecular_function	endopeptidase activator activity
G	0061702	cellular_component	canonical inflammasome complex
G	0070269	biological_process	pyroptotic inflammatory response
G	0072557	cellular_component	IPAF inflammasome complex
G	0089720	molecular_function	caspase binding
H	0000166	molecular_function	nucleotide binding
H	0002218	biological_process	activation of innate immune response
H	0002376	biological_process	immune system process
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0006915	biological_process	apoptotic process
H	0006954	biological_process	inflammatory response
H	0010954	biological_process	positive regulation of protein processing
H	0016045	biological_process	detection of bacterium
H	0032731	biological_process	positive regulation of interleukin-1 beta production
H	0042742	biological_process	defense response to bacterium
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0042981	biological_process	regulation of apoptotic process
H	0043065	biological_process	positive regulation of apoptotic process
H	0045087	biological_process	innate immune response
H	0051260	biological_process	protein homooligomerization
H	0061133	molecular_function	endopeptidase activator activity
H	0061702	cellular_component	canonical inflammasome complex
H	0070269	biological_process	pyroptotic inflammatory response
H	0072557	cellular_component	IPAF inflammasome complex
H	0089720	molecular_function	caspase binding
I	0000166	molecular_function	nucleotide binding
I	0002218	biological_process	activation of innate immune response
I	0002376	biological_process	immune system process
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005829	cellular_component	cytosol
I	0005886	cellular_component	plasma membrane
I	0006915	biological_process	apoptotic process
I	0006954	biological_process	inflammatory response
I	0010954	biological_process	positive regulation of protein processing
I	0016045	biological_process	detection of bacterium
I	0032731	biological_process	positive regulation of interleukin-1 beta production
I	0042742	biological_process	defense response to bacterium
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0042981	biological_process	regulation of apoptotic process
I	0043065	biological_process	positive regulation of apoptotic process
I	0045087	biological_process	innate immune response
I	0051260	biological_process	protein homooligomerization
I	0061133	molecular_function	endopeptidase activator activity
I	0061702	cellular_component	canonical inflammasome complex
I	0070269	biological_process	pyroptotic inflammatory response
I	0072557	cellular_component	IPAF inflammasome complex
I	0089720	molecular_function	caspase binding
J	0000166	molecular_function	nucleotide binding
J	0002218	biological_process	activation of innate immune response
J	0002376	biological_process	immune system process
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005829	cellular_component	cytosol
J	0005886	cellular_component	plasma membrane
J	0006915	biological_process	apoptotic process
J	0006954	biological_process	inflammatory response
J	0010954	biological_process	positive regulation of protein processing
J	0016045	biological_process	detection of bacterium
J	0032731	biological_process	positive regulation of interleukin-1 beta production
J	0042742	biological_process	defense response to bacterium
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0042981	biological_process	regulation of apoptotic process
J	0043065	biological_process	positive regulation of apoptotic process
J	0045087	biological_process	innate immune response
J	0051260	biological_process	protein homooligomerization
J	0061133	molecular_function	endopeptidase activator activity
J	0061702	cellular_component	canonical inflammasome complex
J	0070269	biological_process	pyroptotic inflammatory response
J	0072557	cellular_component	IPAF inflammasome complex
J	0089720	molecular_function	caspase binding
K	0000166	molecular_function	nucleotide binding
K	0002218	biological_process	activation of innate immune response
K	0002376	biological_process	immune system process
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005829	cellular_component	cytosol
K	0005886	cellular_component	plasma membrane
K	0006915	biological_process	apoptotic process
K	0006954	biological_process	inflammatory response
K	0010954	biological_process	positive regulation of protein processing
K	0016045	biological_process	detection of bacterium
K	0032731	biological_process	positive regulation of interleukin-1 beta production
K	0042742	biological_process	defense response to bacterium
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0042981	biological_process	regulation of apoptotic process
K	0043065	biological_process	positive regulation of apoptotic process
K	0045087	biological_process	innate immune response
K	0051260	biological_process	protein homooligomerization
K	0061133	molecular_function	endopeptidase activator activity
K	0061702	cellular_component	canonical inflammasome complex
K	0070269	biological_process	pyroptotic inflammatory response
K	0072557	cellular_component	IPAF inflammasome complex
K	0089720	molecular_function	caspase binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3443
Details	Domain: {"description":"NACHT","evidences":[{"source":"PROSITE-ProRule","id":"PRU00136","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	220
Details	Repeat: {"description":"LRR 1","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	253
Details	Repeat: {"description":"LRR 2","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	253
Details	Repeat: {"description":"LRR 3","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	253
Details	Repeat: {"description":"LRR 4","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	275
Details	Repeat: {"description":"LRR 5","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	253
Details	Repeat: {"description":"LRR 6","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	242
Details	Repeat: {"description":"LRR 7","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	264
Details	Repeat: {"description":"LRR 8","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	242
Details	Repeat: {"description":"LRR 9","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	297
Details	Repeat: {"description":"LRR 10","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	220
Details	Repeat: {"description":"LRR 11","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2233
Details	Region: {"description":"Nucleotide-binding domain (NBD)"}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1177
Details	Region: {"description":"Winged-helix domain (WHD)"}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	77
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4KXF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	11
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22885697","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)