3JBL

Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002218	biological_process	activation of innate immune response
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006915	biological_process	apoptotic process
A	0006954	biological_process	inflammatory response
A	0016045	biological_process	detection of bacterium
A	0032731	biological_process	positive regulation of interleukin-1 beta production
A	0042742	biological_process	defense response to bacterium
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0042981	biological_process	regulation of apoptotic process
A	0045087	biological_process	innate immune response
A	0051260	biological_process	protein homooligomerization
A	0061702	cellular_component	canonical inflammasome complex
A	0070269	biological_process	pyroptotic inflammatory response
A	0072557	cellular_component	IPAF inflammasome complex
B	0002218	biological_process	activation of innate immune response
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006915	biological_process	apoptotic process
B	0006954	biological_process	inflammatory response
B	0016045	biological_process	detection of bacterium
B	0032731	biological_process	positive regulation of interleukin-1 beta production
B	0042742	biological_process	defense response to bacterium
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0042981	biological_process	regulation of apoptotic process
B	0045087	biological_process	innate immune response
B	0051260	biological_process	protein homooligomerization
B	0061702	cellular_component	canonical inflammasome complex
B	0070269	biological_process	pyroptotic inflammatory response
B	0072557	cellular_component	IPAF inflammasome complex
C	0002218	biological_process	activation of innate immune response
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006915	biological_process	apoptotic process
C	0006954	biological_process	inflammatory response
C	0016045	biological_process	detection of bacterium
C	0032731	biological_process	positive regulation of interleukin-1 beta production
C	0042742	biological_process	defense response to bacterium
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0042981	biological_process	regulation of apoptotic process
C	0045087	biological_process	innate immune response
C	0051260	biological_process	protein homooligomerization
C	0061702	cellular_component	canonical inflammasome complex
C	0070269	biological_process	pyroptotic inflammatory response
C	0072557	cellular_component	IPAF inflammasome complex
D	0002218	biological_process	activation of innate immune response
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006915	biological_process	apoptotic process
D	0006954	biological_process	inflammatory response
D	0016045	biological_process	detection of bacterium
D	0032731	biological_process	positive regulation of interleukin-1 beta production
D	0042742	biological_process	defense response to bacterium
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0042981	biological_process	regulation of apoptotic process
D	0045087	biological_process	innate immune response
D	0051260	biological_process	protein homooligomerization
D	0061702	cellular_component	canonical inflammasome complex
D	0070269	biological_process	pyroptotic inflammatory response
D	0072557	cellular_component	IPAF inflammasome complex
E	0002218	biological_process	activation of innate immune response
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006915	biological_process	apoptotic process
E	0006954	biological_process	inflammatory response
E	0016045	biological_process	detection of bacterium
E	0032731	biological_process	positive regulation of interleukin-1 beta production
E	0042742	biological_process	defense response to bacterium
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0042981	biological_process	regulation of apoptotic process
E	0045087	biological_process	innate immune response
E	0051260	biological_process	protein homooligomerization
E	0061702	cellular_component	canonical inflammasome complex
E	0070269	biological_process	pyroptotic inflammatory response
E	0072557	cellular_component	IPAF inflammasome complex
F	0002218	biological_process	activation of innate immune response
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006915	biological_process	apoptotic process
F	0006954	biological_process	inflammatory response
F	0016045	biological_process	detection of bacterium
F	0032731	biological_process	positive regulation of interleukin-1 beta production
F	0042742	biological_process	defense response to bacterium
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0042981	biological_process	regulation of apoptotic process
F	0045087	biological_process	innate immune response
F	0051260	biological_process	protein homooligomerization
F	0061702	cellular_component	canonical inflammasome complex
F	0070269	biological_process	pyroptotic inflammatory response
F	0072557	cellular_component	IPAF inflammasome complex
G	0002218	biological_process	activation of innate immune response
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006915	biological_process	apoptotic process
G	0006954	biological_process	inflammatory response
G	0016045	biological_process	detection of bacterium
G	0032731	biological_process	positive regulation of interleukin-1 beta production
G	0042742	biological_process	defense response to bacterium
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0042981	biological_process	regulation of apoptotic process
G	0045087	biological_process	innate immune response
G	0051260	biological_process	protein homooligomerization
G	0061702	cellular_component	canonical inflammasome complex
G	0070269	biological_process	pyroptotic inflammatory response
G	0072557	cellular_component	IPAF inflammasome complex
H	0002218	biological_process	activation of innate immune response
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006915	biological_process	apoptotic process
H	0006954	biological_process	inflammatory response
H	0016045	biological_process	detection of bacterium
H	0032731	biological_process	positive regulation of interleukin-1 beta production
H	0042742	biological_process	defense response to bacterium
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0042981	biological_process	regulation of apoptotic process
H	0045087	biological_process	innate immune response
H	0051260	biological_process	protein homooligomerization
H	0061702	cellular_component	canonical inflammasome complex
H	0070269	biological_process	pyroptotic inflammatory response
H	0072557	cellular_component	IPAF inflammasome complex
I	0002218	biological_process	activation of innate immune response
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006915	biological_process	apoptotic process
I	0006954	biological_process	inflammatory response
I	0016045	biological_process	detection of bacterium
I	0032731	biological_process	positive regulation of interleukin-1 beta production
I	0042742	biological_process	defense response to bacterium
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0042981	biological_process	regulation of apoptotic process
I	0045087	biological_process	innate immune response
I	0051260	biological_process	protein homooligomerization
I	0061702	cellular_component	canonical inflammasome complex
I	0070269	biological_process	pyroptotic inflammatory response
I	0072557	cellular_component	IPAF inflammasome complex
J	0002218	biological_process	activation of innate immune response
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006915	biological_process	apoptotic process
J	0006954	biological_process	inflammatory response
J	0016045	biological_process	detection of bacterium
J	0032731	biological_process	positive regulation of interleukin-1 beta production
J	0042742	biological_process	defense response to bacterium
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0042981	biological_process	regulation of apoptotic process
J	0045087	biological_process	innate immune response
J	0051260	biological_process	protein homooligomerization
J	0061702	cellular_component	canonical inflammasome complex
J	0070269	biological_process	pyroptotic inflammatory response
J	0072557	cellular_component	IPAF inflammasome complex
K	0002218	biological_process	activation of innate immune response
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006915	biological_process	apoptotic process
K	0006954	biological_process	inflammatory response
K	0016045	biological_process	detection of bacterium
K	0032731	biological_process	positive regulation of interleukin-1 beta production
K	0042742	biological_process	defense response to bacterium
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0042981	biological_process	regulation of apoptotic process
K	0045087	biological_process	innate immune response
K	0051260	biological_process	protein homooligomerization
K	0061702	cellular_component	canonical inflammasome complex
K	0070269	biological_process	pyroptotic inflammatory response
K	0072557	cellular_component	IPAF inflammasome complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	33
Details	BINDING: BINDING => ECO:0000269|PubMed:23765277, ECO:0007744|PDB:4KXF

Chain	Residue	Details
K	THR135
C	THR135
C	GLY172
C	HIS443
D	THR135
D	GLY172
D	HIS443
E	THR135
E	GLY172
E	HIS443
F	THR135
K	GLY172
F	GLY172
F	HIS443
G	THR135
G	GLY172
G	HIS443
H	THR135
H	GLY172
H	HIS443
I	THR135
I	GLY172
K	HIS443
I	HIS443
J	THR135
J	GLY172
J	HIS443
A	THR135
A	GLY172
A	HIS443
B	THR135
B	GLY172
B	HIS443

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site_id	SWS_FT_FI2
Number of Residues	11
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:22885697, ECO:0000269|PubMed:23765277

Chain	Residue	Details
K	SEP533
I	SEP533
J	SEP533
A	SEP533
B	SEP533
C	SEP533
D	SEP533
E	SEP533
F	SEP533
G	SEP533
H	SEP533

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