3JBL
Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot for one second before plunging |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 9113 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.7 |
| Software | EMAN,RELION,SPIDER |
| Symmetry | C11 |
