3EQF
X-ray structure of the human mitogen-activated protein kinase kinase 1 (MEK1) in a binary complex with K252A and MG2P
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000165 | biological_process | MAPK cascade |
A | 0000187 | biological_process | obsolete activation of MAPK activity |
A | 0004672 | molecular_function | protein kinase activity |
A | 0004674 | molecular_function | protein serine/threonine kinase activity |
A | 0004708 | molecular_function | MAP kinase kinase activity |
A | 0004712 | molecular_function | protein serine/threonine/tyrosine kinase activity |
A | 0004713 | molecular_function | protein tyrosine kinase activity |
A | 0005078 | molecular_function | MAP-kinase scaffold activity |
A | 0005515 | molecular_function | protein binding |
A | 0005524 | molecular_function | ATP binding |
A | 0005634 | cellular_component | nucleus |
A | 0005737 | cellular_component | cytoplasm |
A | 0005739 | cellular_component | mitochondrion |
A | 0005769 | cellular_component | early endosome |
A | 0005770 | cellular_component | late endosome |
A | 0005783 | cellular_component | endoplasmic reticulum |
A | 0005794 | cellular_component | Golgi apparatus |
A | 0005813 | cellular_component | centrosome |
A | 0005816 | cellular_component | spindle pole body |
A | 0005829 | cellular_component | cytosol |
A | 0005856 | cellular_component | cytoskeleton |
A | 0005886 | cellular_component | plasma membrane |
A | 0005925 | cellular_component | focal adhesion |
A | 0006468 | biological_process | protein phosphorylation |
A | 0006935 | biological_process | chemotaxis |
A | 0007165 | biological_process | signal transduction |
A | 0007507 | biological_process | heart development |
A | 0008285 | biological_process | negative regulation of cell population proliferation |
A | 0010628 | biological_process | positive regulation of gene expression |
A | 0014044 | biological_process | Schwann cell development |
A | 0016020 | cellular_component | membrane |
A | 0021697 | biological_process | cerebellar cortex formation |
A | 0030182 | biological_process | neuron differentiation |
A | 0030216 | biological_process | keratinocyte differentiation |
A | 0030295 | molecular_function | protein kinase activator activity |
A | 0030878 | biological_process | thyroid gland development |
A | 0032872 | biological_process | regulation of stress-activated MAPK cascade |
A | 0035987 | biological_process | endodermal cell differentiation |
A | 0038133 | biological_process | ERBB2-ERBB3 signaling pathway |
A | 0042552 | biological_process | myelination |
A | 0043410 | biological_process | positive regulation of MAPK cascade |
A | 0043539 | molecular_function | protein serine/threonine kinase activator activity |
A | 0044342 | biological_process | type B pancreatic cell proliferation |
A | 0045893 | biological_process | positive regulation of DNA-templated transcription |
A | 0048009 | biological_process | insulin-like growth factor receptor signaling pathway |
A | 0048538 | biological_process | thymus development |
A | 0048679 | biological_process | regulation of axon regeneration |
A | 0048870 | biological_process | cell motility |
A | 0050772 | biological_process | positive regulation of axonogenesis |
A | 0060020 | biological_process | Bergmann glial cell differentiation |
A | 0060324 | biological_process | face development |
A | 0060425 | biological_process | lung morphogenesis |
A | 0060440 | biological_process | trachea formation |
A | 0060502 | biological_process | epithelial cell proliferation involved in lung morphogenesis |
A | 0060674 | biological_process | placenta blood vessel development |
A | 0060711 | biological_process | labyrinthine layer development |
A | 0070371 | biological_process | ERK1 and ERK2 cascade |
A | 0070374 | biological_process | positive regulation of ERK1 and ERK2 cascade |
A | 0071902 | biological_process | positive regulation of protein serine/threonine kinase activity |
A | 0090170 | biological_process | regulation of Golgi inheritance |
A | 0090398 | biological_process | cellular senescence |
A | 0097110 | molecular_function | scaffold protein binding |
A | 0106310 | molecular_function | protein serine kinase activity |
A | 1903226 | biological_process | positive regulation of endodermal cell differentiation |
A | 2000641 | biological_process | regulation of early endosome to late endosome transport |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 15 |
Details | BINDING SITE FOR RESIDUE KSA A 1 |
Chain | Residue |
A | LEU74 |
A | HIS145 |
A | MET146 |
A | GLY149 |
A | GLN153 |
A | SER194 |
A | LEU197 |
A | GLY75 |
A | ALA76 |
A | GLY77 |
A | VAL82 |
A | ALA95 |
A | LYS97 |
A | MET143 |
A | GLU144 |
site_id | AC2 |
Number of Residues | 2 |
Details | BINDING SITE FOR RESIDUE MG A 3 |
Chain | Residue |
A | ASP190 |
A | ASP208 |
site_id | AC3 |
Number of Residues | 2 |
Details | BINDING SITE FOR RESIDUE CA A 5 |
Chain | Residue |
A | ASP65 |
A | ASP66 |
site_id | AC4 |
Number of Residues | 2 |
Details | BINDING SITE FOR RESIDUE NA A 6 |
Chain | Residue |
A | ASP65 |
A | ASP66 |
Functional Information from PROSITE/UniProt
site_id | PS00107 |
Number of Residues | 24 |
Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK |
Chain | Residue | Details |
A | LEU74-LYS97 |
site_id | PS00108 |
Number of Residues | 13 |
Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV |
Chain | Residue | Details |
A | ILE186-VAL198 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027 |
Chain | Residue | Details |
A | ASP190 |
site_id | SWS_FT_FI2 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000269|PubMed:15543157, ECO:0000269|PubMed:17880056, ECO:0000269|PubMed:18951019, ECO:0000269|PubMed:19019675, ECO:0000269|PubMed:19706763, ECO:0000269|PubMed:21310613 |
Chain | Residue | Details |
A | LEU74 | |
A | MET143 | |
A | SER150 | |
A | LYS192 | |
A | ASP208 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:19161339, ECO:0007744|PDB:3EQH |
Chain | Residue | Details |
A | LYS97 |
site_id | SWS_FT_FI4 |
Number of Residues | 2 |
Details | BINDING: BINDING => ECO:0000269|PubMed:19161339, ECO:0007744|PDB:3EQF |
Chain | Residue | Details |
A | GLU144 | |
A | SER194 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:10409742, ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746 |
Chain | Residue | Details |
A | SER218 |
site_id | SWS_FT_FI6 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746 |
Chain | Residue | Details |
A | SER222 |
site_id | SWS_FT_FI7 |
Number of Residues | 1 |
Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18691976 |
Chain | Residue | Details |
A | THR286 |
site_id | SWS_FT_FI8 |
Number of Residues | 1 |
Details | MOD_RES: Phosphothreonine; by MAPK1 => ECO:0000250|UniProtKB:Q01986 |
Chain | Residue | Details |
A | THR292 |
site_id | SWS_FT_FI9 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine; by PAK => ECO:0000269|PubMed:16129686 |
Chain | Residue | Details |
A | ASN298 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 1ir3 |
Chain | Residue | Details |
A | SER194 | |
A | ASP190 |
site_id | CSA2 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 1ir3 |
Chain | Residue | Details |
A | LYS192 | |
A | ASP190 |
site_id | CSA3 |
Number of Residues | 3 |
Details | Annotated By Reference To The Literature 1ir3 |
Chain | Residue | Details |
A | LYS192 | |
A | THR226 | |
A | ASP190 |
site_id | CSA4 |
Number of Residues | 3 |
Details | Annotated By Reference To The Literature 1ir3 |
Chain | Residue | Details |
A | LYS192 | |
A | ASN195 | |
A | ASP190 |