3EPP

Crystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefungin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0006370	biological_process	7-methylguanosine mRNA capping
B	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
B	0006370	biological_process	7-methylguanosine mRNA capping

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE SFG A 401

Chain	Residue
A	LYS17
A	PHE122
A	VAL123
A	TYR126
A	MET137
A	HOH427
A	HOH444
A	GLY42
A	GLY44
A	ASP64
A	ILE65
A	ASP98
A	SER99
A	SER100
A	GLN121

---

site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE SFG B 401

Chain	Residue
B	LYS17
B	GLY42
B	GLY44
B	ASP64
B	ILE65
B	ASP98
B	SER99
B	GLN121
B	PHE122
B	VAL123
B	TYR126
B	MET137
B	HOH430
B	HOH474

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00895

Chain	Residue	Details
A	ASN13
B	ASN13

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00895, ECO:0000269|PubMed:27422871, ECO:0007744|PDB:3BGV, ECO:0007744|PDB:5E9J, ECO:0007744|PDB:5E9W

Chain	Residue	Details
A	LYS17
A	GLY42
A	ASP64
B	LYS17
B	GLY42
B	ASP64

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:27422871, ECO:0007744|PDB:3BGV, ECO:0007744|PDB:5E9J, ECO:0007744|PDB:5E9W

Chain	Residue	Details
A	ASP98
A	GLN121
A	TYR126
B	ASP98
B	GLN121
B	TYR126

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00895

Chain	Residue	Details
A	LYS45
B	HIS125
B	GLU207
B	TYR304
A	LYS51
A	ARG76
A	HIS125
A	GLU207
A	TYR304
B	LYS45
B	LYS51
B	ARG76

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