2Z1W

tRNA guanine transglycosylase TGT E235Q mutant in complex with BDI (2-BUTYL-5,6-DIHYDRO-1H-IMIDAZO[4,5-D]PYRIDAZINE-4,7-DIONE)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006400	biological_process	tRNA modification
A	0008033	biological_process	tRNA processing
A	0008479	molecular_function	tRNA-guanosine(34) queuine transglycosylase activity
A	0008616	biological_process	queuosine biosynthetic process
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0046872	molecular_function	metal ion binding
A	0101030	biological_process	tRNA-guanine transglycosylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 500

Chain	Residue
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

site_id	AC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE BDI A 600

Chain	Residue
A	GLY230
A	MET260
A	GOL703
A	HOH961
A	HOH963
A	HOH964
A	TYR106
A	ASP156
A	CYS158
A	GLN203
A	GLY229

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 702

Chain	Residue
A	PRO56
A	GLU57
A	GLY94
A	TRP95
A	ASP96
A	ARG97
A	HOH1000

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 703

Chain	Residue
A	VAL45
A	ASN70
A	ASP102
A	GLN107
A	TYR258
A	ASP280
A	CYS281
A	BDI600
A	HOH952

---

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 705

Chain	Residue
A	ARG34
A	HIS145
A	GLY148
A	SER149
A	GLU191
A	GLN192
A	GOL708
A	HOH733
A	HOH761

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 706

Chain	Residue
A	GLU317
A	LYS360
A	PHE377
A	ARG380
A	HOH779
A	HOH894

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 708

Chain	Residue
A	ARG34
A	GLY87
A	GLY88
A	GLY148
A	GOL705
A	HOH770
A	HOH806

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 710

Chain	Residue
A	ALA61
A	CYS320
A	ALA352
A	GLN356
A	HOH734
A	HOH872
A	HOH922

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 715

Chain	Residue
A	MET176
A	LYS180
A	ALA217
A	GLU220
A	ILE221
A	HOH869

---

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 716

Chain	Residue
A	LYS24
A	GLU273
A	HOH1008

---

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 717

Chain	Residue
A	PRO249
A	MET250
A	LEU251
A	PRO252
A	ASP253

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	ASP102

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	ASP280

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:12949492

Chain	Residue	Details
A	ASP102
A	ASP156
A	GLN203
A	GLY230

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936

Chain	Residue	Details
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1pud

Chain	Residue	Details
A	ASP102

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 881

Chain	Residue	Details
A	ASP102	proton shuttle (general acid/base)
A	ASP280	covalent catalysis
A	CYS318	metal ligand
A	CYS320	metal ligand
A	CYS323	metal ligand
A	HIS349	metal ligand

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