2WJ1
3D-crystal structure of humanized-rat fatty acid amide hydrolase (FAAH) conjugated with 7-phenyl-1-(4-(pyridin-2-yl)oxazol-2-yl)heptan- 1-one, an alpha-ketooxazole
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000139 | cellular_component | Golgi membrane |
A | 0004040 | molecular_function | amidase activity |
A | 0005515 | molecular_function | protein binding |
A | 0005543 | molecular_function | phospholipid binding |
A | 0005783 | cellular_component | endoplasmic reticulum |
A | 0005789 | cellular_component | endoplasmic reticulum membrane |
A | 0005794 | cellular_component | Golgi apparatus |
A | 0006631 | biological_process | fatty acid metabolic process |
A | 0008289 | molecular_function | lipid binding |
A | 0009062 | biological_process | fatty acid catabolic process |
A | 0016020 | cellular_component | membrane |
A | 0016042 | biological_process | lipid catabolic process |
A | 0016787 | molecular_function | hydrolase activity |
A | 0016788 | molecular_function | hydrolase activity, acting on ester bonds |
A | 0017064 | molecular_function | fatty acid amide hydrolase activity |
A | 0031090 | cellular_component | organelle membrane |
A | 0042802 | molecular_function | identical protein binding |
A | 0045907 | biological_process | positive regulation of vasoconstriction |
A | 0047372 | molecular_function | acylglycerol lipase activity |
A | 0052651 | biological_process | monoacylglycerol catabolic process |
A | 0098793 | cellular_component | presynapse |
A | 0098794 | cellular_component | postsynapse |
A | 0098978 | cellular_component | glutamatergic synapse |
A | 0150036 | biological_process | regulation of trans-synaptic signaling by endocannabinoid, modulating synaptic transmission |
B | 0000139 | cellular_component | Golgi membrane |
B | 0004040 | molecular_function | amidase activity |
B | 0005515 | molecular_function | protein binding |
B | 0005543 | molecular_function | phospholipid binding |
B | 0005783 | cellular_component | endoplasmic reticulum |
B | 0005789 | cellular_component | endoplasmic reticulum membrane |
B | 0005794 | cellular_component | Golgi apparatus |
B | 0006631 | biological_process | fatty acid metabolic process |
B | 0008289 | molecular_function | lipid binding |
B | 0009062 | biological_process | fatty acid catabolic process |
B | 0016020 | cellular_component | membrane |
B | 0016042 | biological_process | lipid catabolic process |
B | 0016787 | molecular_function | hydrolase activity |
B | 0016788 | molecular_function | hydrolase activity, acting on ester bonds |
B | 0017064 | molecular_function | fatty acid amide hydrolase activity |
B | 0031090 | cellular_component | organelle membrane |
B | 0042802 | molecular_function | identical protein binding |
B | 0045907 | biological_process | positive regulation of vasoconstriction |
B | 0047372 | molecular_function | acylglycerol lipase activity |
B | 0052651 | biological_process | monoacylglycerol catabolic process |
B | 0098793 | cellular_component | presynapse |
B | 0098794 | cellular_component | postsynapse |
B | 0098978 | cellular_component | glutamatergic synapse |
B | 0150036 | biological_process | regulation of trans-synaptic signaling by endocannabinoid, modulating synaptic transmission |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 15 |
Details | BINDING SITE FOR RESIDUE S99 A 1577 |
Chain | Residue |
A | SER190 |
A | GLY240 |
A | SER241 |
A | CYS269 |
A | LEU278 |
A | THR488 |
A | HOH2120 |
A | MET191 |
A | PHE192 |
A | SER193 |
A | SER217 |
A | THR236 |
A | ASP237 |
A | ILE238 |
A | GLY239 |
site_id | AC2 |
Number of Residues | 14 |
Details | BINDING SITE FOR RESIDUE S99 B 1578 |
Chain | Residue |
B | SER190 |
B | MET191 |
B | PHE192 |
B | SER217 |
B | THR236 |
B | ASP237 |
B | ILE238 |
B | GLY239 |
B | GLY240 |
B | SER241 |
B | CYS269 |
B | LEU278 |
B | THR488 |
B | HOH2098 |
site_id | AC3 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE CL A 1578 |
Chain | Residue |
A | ASN259 |
A | HOH2110 |
B | ASN259 |
Functional Information from PROSITE/UniProt
site_id | PS00571 |
Number of Residues | 32 |
Details | AMIDASES Amidases signature. GGSSGGeGAlIGsggsplGlGtDiGgSIRfPS |
Chain | Residue | Details |
A | GLY215-SER246 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1036 |
Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
Chain | Residue | Details |
A | ARG30-ASP403 | |
A | ASN434-SER579 | |
B | ARG30-ASP403 | |
B | ASN434-SER579 |
site_id | SWS_FT_FI2 |
Number of Residues | 58 |
Details | INTRAMEM: INTRAMEM => ECO:0000305 |
Chain | Residue | Details |
A | LEU404-LEU433 | |
B | LEU404-LEU433 |
site_id | SWS_FT_FI3 |
Number of Residues | 4 |
Details | ACT_SITE: Charge relay system |
Chain | Residue | Details |
A | LYS142 | |
A | SER217 | |
B | LYS142 | |
B | SER217 |
site_id | SWS_FT_FI4 |
Number of Residues | 2 |
Details | ACT_SITE: Acyl-ester intermediate |
Chain | Residue | Details |
A | SER241 | |
B | SER241 |
site_id | SWS_FT_FI5 |
Number of Residues | 6 |
Details | BINDING: |
Chain | Residue | Details |
A | MET191 | |
A | SER217 | |
A | ILE238 | |
B | MET191 | |
B | SER217 | |
B | ILE238 |
site_id | SWS_FT_FI6 |
Number of Residues | 2 |
Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:O00519 |
Chain | Residue | Details |
A | SER241 | |
B | SER241 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 7 |
Details | M-CSA 731 |
Chain | Residue | Details |
A | LYS142 | proton acceptor, proton donor |
A | SER217 | proton acceptor, proton donor, proton relay |
A | SER218 | electrostatic stabiliser |
A | ILE238 | electrostatic stabiliser |
A | GLY239 | electrostatic stabiliser |
A | GLY240 | electrostatic stabiliser |
A | SER241 | nucleofuge, nucleophile, proton acceptor, proton donor |
site_id | MCSA2 |
Number of Residues | 7 |
Details | M-CSA 731 |
Chain | Residue | Details |
B | LYS142 | proton acceptor, proton donor |
B | SER217 | proton acceptor, proton donor, proton relay |
B | SER218 | electrostatic stabiliser |
B | ILE238 | electrostatic stabiliser |
B | GLY239 | electrostatic stabiliser |
B | GLY240 | electrostatic stabiliser |
B | SER241 | nucleofuge, nucleophile, proton acceptor, proton donor |