2PD6
Structure of human hydroxysteroid dehydrogenase type 8, HSD17B8
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004303 | molecular_function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005740 | cellular_component | mitochondrial envelope |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006631 | biological_process | fatty acid metabolic process |
| A | 0006633 | biological_process | fatty acid biosynthetic process |
| A | 0006694 | biological_process | steroid biosynthetic process |
| A | 0006703 | biological_process | estrogen biosynthetic process |
| A | 0008209 | biological_process | androgen metabolic process |
| A | 0008210 | biological_process | estrogen metabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0047035 | molecular_function | testosterone dehydrogenase (NAD+) activity |
| A | 0048038 | molecular_function | quinone binding |
| A | 0051290 | biological_process | protein heterotetramerization |
| A | 0070404 | molecular_function | NADH binding |
| A | 0106386 | molecular_function | (3R)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| A | 1990204 | cellular_component | oxidoreductase complex |
| B | 0004303 | molecular_function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005740 | cellular_component | mitochondrial envelope |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006631 | biological_process | fatty acid metabolic process |
| B | 0006633 | biological_process | fatty acid biosynthetic process |
| B | 0006694 | biological_process | steroid biosynthetic process |
| B | 0006703 | biological_process | estrogen biosynthetic process |
| B | 0008209 | biological_process | androgen metabolic process |
| B | 0008210 | biological_process | estrogen metabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0047035 | molecular_function | testosterone dehydrogenase (NAD+) activity |
| B | 0048038 | molecular_function | quinone binding |
| B | 0051290 | biological_process | protein heterotetramerization |
| B | 0070404 | molecular_function | NADH binding |
| B | 0106386 | molecular_function | (3R)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| B | 1990204 | cellular_component | oxidoreductase complex |
| C | 0004303 | molecular_function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005740 | cellular_component | mitochondrial envelope |
| C | 0005759 | cellular_component | mitochondrial matrix |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006629 | biological_process | lipid metabolic process |
| C | 0006631 | biological_process | fatty acid metabolic process |
| C | 0006633 | biological_process | fatty acid biosynthetic process |
| C | 0006694 | biological_process | steroid biosynthetic process |
| C | 0006703 | biological_process | estrogen biosynthetic process |
| C | 0008209 | biological_process | androgen metabolic process |
| C | 0008210 | biological_process | estrogen metabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0047035 | molecular_function | testosterone dehydrogenase (NAD+) activity |
| C | 0048038 | molecular_function | quinone binding |
| C | 0051290 | biological_process | protein heterotetramerization |
| C | 0070404 | molecular_function | NADH binding |
| C | 0106386 | molecular_function | (3R)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| C | 1990204 | cellular_component | oxidoreductase complex |
| D | 0004303 | molecular_function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005740 | cellular_component | mitochondrial envelope |
| D | 0005759 | cellular_component | mitochondrial matrix |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0006629 | biological_process | lipid metabolic process |
| D | 0006631 | biological_process | fatty acid metabolic process |
| D | 0006633 | biological_process | fatty acid biosynthetic process |
| D | 0006694 | biological_process | steroid biosynthetic process |
| D | 0006703 | biological_process | estrogen biosynthetic process |
| D | 0008209 | biological_process | androgen metabolic process |
| D | 0008210 | biological_process | estrogen metabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0047035 | molecular_function | testosterone dehydrogenase (NAD+) activity |
| D | 0048038 | molecular_function | quinone binding |
| D | 0051290 | biological_process | protein heterotetramerization |
| D | 0070404 | molecular_function | NADH binding |
| D | 0106386 | molecular_function | (3R)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| D | 1990204 | cellular_component | oxidoreductase complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAD A 1 |
| Chain | Residue |
| A | GLY18 |
| A | CYS103 |
| A | ALA104 |
| A | VAL126 |
| A | ILE154 |
| A | SER155 |
| A | SER156 |
| A | TYR169 |
| A | LYS173 |
| A | PRO199 |
| A | GLY200 |
| A | SER21 |
| A | ILE202 |
| A | THR204 |
| A | PRO205 |
| A | MET206 |
| A | HOH278 |
| A | HOH285 |
| A | HOH337 |
| A | GLY22 |
| A | ILE23 |
| A | ASP42 |
| A | LEU43 |
| A | ALA74 |
| A | ASP75 |
| A | VAL76 |
| site_id | AC2 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE NAD B 2 |
| Chain | Residue |
| B | GLY18 |
| B | SER21 |
| B | GLY22 |
| B | ILE23 |
| B | ASP42 |
| B | LEU43 |
| B | ALA74 |
| B | ASP75 |
| B | VAL76 |
| B | CYS103 |
| B | ALA104 |
| B | GLY105 |
| B | ILE154 |
| B | SER155 |
| B | SER156 |
| B | TYR169 |
| B | LYS173 |
| B | PRO199 |
| B | GLY200 |
| B | ILE202 |
| B | THR204 |
| B | PRO205 |
| B | MET206 |
| B | THR207 |
| site_id | AC3 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE NAD C 3 |
| Chain | Residue |
| C | GLY18 |
| C | SER21 |
| C | GLY22 |
| C | ILE23 |
| C | ASP42 |
| C | LEU43 |
| C | ALA74 |
| C | ASP75 |
| C | VAL76 |
| C | CYS103 |
| C | ILE154 |
| C | SER155 |
| C | TYR169 |
| C | LYS173 |
| C | PRO199 |
| C | GLY200 |
| C | ILE202 |
| C | THR204 |
| C | PRO205 |
| C | MET206 |
| C | THR207 |
| C | HOH288 |
| C | HOH306 |
| site_id | AC4 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAD D 4 |
| Chain | Residue |
| D | GLY18 |
| D | SER21 |
| D | GLY22 |
| D | ILE23 |
| D | ASP42 |
| D | LEU43 |
| D | ALA74 |
| D | ASP75 |
| D | VAL76 |
| D | CYS103 |
| D | ALA104 |
| D | GLY105 |
| D | ILE154 |
| D | SER155 |
| D | SER156 |
| D | TYR169 |
| D | LYS173 |
| D | PRO199 |
| D | GLY200 |
| D | ILE202 |
| D | THR204 |
| D | PRO205 |
| D | MET206 |
| D | THR207 |
| D | HOH282 |
| D | HOH291 |
Functional Information from PROSITE/UniProt
| site_id | PS00061 |
| Number of Residues | 29 |
| Details | ADH_SHORT Short-chain dehydrogenases/reductases family signature. SivgkvgnvgQtnYAASKAGViGLTqTAA |
| Chain | Residue | Details |
| A | SER156-ALA184 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10001 |
| Chain | Residue | Details |
| A | TYR169 | |
| B | TYR169 | |
| C | TYR169 | |
| D | TYR169 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:25203508, ECO:0000269|Ref.12, ECO:0007744|PDB:2PD6, ECO:0007744|PDB:4CQL |
| Chain | Residue | Details |
| A | LEU15 | |
| C | ASP42 | |
| C | ALA74 | |
| C | TYR169 | |
| D | LEU15 | |
| D | ASP42 | |
| D | ALA74 | |
| D | TYR169 | |
| A | ASP42 | |
| A | ALA74 | |
| A | TYR169 | |
| B | LEU15 | |
| B | ASP42 | |
| B | ALA74 | |
| B | TYR169 | |
| C | LEU15 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | SER156 | |
| B | SER156 | |
| C | SER156 | |
| D | SER156 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|Ref.12, ECO:0007744|PDB:2PD6, ECO:0007744|PDB:4CQM |
| Chain | Residue | Details |
| A | ILE202 | |
| B | ILE202 | |
| C | ILE202 | |
| D | ILE202 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P50171 |
| Chain | Residue | Details |
| A | SER60 | |
| B | SER60 | |
| C | SER60 | |
| D | SER60 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P50171 |
| Chain | Residue | Details |
| A | LYS160 | |
| A | LYS173 | |
| B | LYS160 | |
| B | LYS173 | |
| C | LYS160 | |
| C | LYS173 | |
| D | LYS160 | |
| D | LYS173 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| A | SER156 | |
| A | TYR169 | |
| A | ASN127 | |
| A | LYS173 |
| site_id | CSA10 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| B | TYR169 | |
| B | LYS173 |
| site_id | CSA11 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| C | TYR169 | |
| C | LYS173 |
| site_id | CSA12 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| D | TYR169 | |
| D | LYS173 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| B | SER156 | |
| B | TYR169 | |
| B | ASN127 | |
| B | LYS173 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| C | SER156 | |
| C | TYR169 | |
| C | ASN127 | |
| C | LYS173 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| D | SER156 | |
| D | TYR169 | |
| D | ASN127 | |
| D | LYS173 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| A | GLN166 | |
| A | LYS173 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| B | GLN166 | |
| B | LYS173 |
| site_id | CSA7 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| C | GLN166 | |
| C | LYS173 |
| site_id | CSA8 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| D | GLN166 | |
| D | LYS173 |
| site_id | CSA9 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ybv |
| Chain | Residue | Details |
| A | TYR169 | |
| A | LYS173 |
