2OZO

Autoinhibited intact human ZAP-70

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001772	cellular_component	immunological synapse
A	0001784	molecular_function	phosphotyrosine residue binding
A	0002250	biological_process	adaptive immune response
A	0002376	biological_process	immune system process
A	0004672	molecular_function	protein kinase activity
A	0004713	molecular_function	protein tyrosine kinase activity
A	0004715	molecular_function	non-membrane spanning protein tyrosine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005911	cellular_component	cell-cell junction
A	0006468	biological_process	protein phosphorylation
A	0006955	biological_process	immune response
A	0007159	biological_process	leukocyte cell-cell adhesion
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0018108	biological_process	peptidyl-tyrosine phosphorylation
A	0019722	biological_process	calcium-mediated signaling
A	0030217	biological_process	T cell differentiation
A	0035556	biological_process	intracellular signal transduction
A	0042101	cellular_component	T cell receptor complex
A	0042110	biological_process	T cell activation
A	0042113	biological_process	B cell activation
A	0043366	biological_process	beta selection
A	0045059	biological_process	positive thymic T cell selection
A	0045060	biological_process	negative thymic T cell selection
A	0045061	biological_process	thymic T cell selection
A	0045121	cellular_component	membrane raft
A	0045582	biological_process	positive regulation of T cell differentiation
A	0046632	biological_process	alpha-beta T cell differentiation
A	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
A	0046641	biological_process	positive regulation of alpha-beta T cell proliferation
A	0050850	biological_process	positive regulation of calcium-mediated signaling
A	0050852	biological_process	T cell receptor signaling pathway
A	0050900	biological_process	leukocyte migration
A	0070489	biological_process	T cell aggregation
A	0072678	biological_process	T cell migration

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 614

Chain	Residue
A	ASN466
A	ASP479
A	ANP615

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ANP A 615

Chain	Residue
A	ALA367
A	LYS369
A	GLU415
A	ALA417
A	PRO421
A	ARG465
A	ASN466
A	LEU468
A	ASP479
A	MG614
A	HOH655
A	HOH671
A	LEU344
A	CYS346
A	GLY347
A	ASN348
A	PHE349
A	VAL352

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	26
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGCGNFGSVRqGvyrmrkkqid........VAIK

Chain	Residue	Details
A	LEU344-LYS369

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	92
Details	Domain: {"description":"SH2 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00191","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	91
Details	Domain: {"description":"SH2 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00191","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	59
Details	Region: {"description":"Interdomain A"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10028","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15292186","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17512407","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"15592455","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by LCK","evidences":[{"source":"PubMed","id":"10037717","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"10037717","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"26903241","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ARG465
A	ASN461

---