2OZO
Autoinhibited intact human ZAP-70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Collection date | 2006-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115879 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.297, 52.929, 69.331 |
| Unit cell angles | 105.91, 92.94, 103.72 |
Refinement procedure
| Resolution | 500.000 - 2.600 |
| R-factor | 0.26 |
| Rwork | 0.220 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 18285 | |
| <I/σ(I)> | 16.18 | 2.1 |
| Completeness [%] | 95.3 | 78.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05 M NaSCN, 21% PEG3350, protein mixed with 9 mM n-decyl-beta-D-thiomaltopyranoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
