2O3E

Crystal structure of engineered neurolysin with thimet oligopeptidase specificity for neurotensin cleavage site.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005758	cellular_component	mitochondrial intermembrane space
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006111	biological_process	regulation of gluconeogenesis
A	0006508	biological_process	proteolysis
A	0006518	biological_process	peptide metabolic process
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0016787	molecular_function	hydrolase activity
A	0030163	biological_process	protein catabolic process
A	0042277	molecular_function	peptide binding
A	0043171	biological_process	peptide catabolic process
A	0046872	molecular_function	metal ion binding
A	0070012	molecular_function	oligopeptidase activity
A	1902809	biological_process	regulation of skeletal muscle fiber differentiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 679

Chain	Residue
A	HIS474
A	HIS478
A	GLU503
A	HOH888

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. TYFHEFGHVM

Chain	Residue	Details
A	THR471-MET480

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10095

Chain	Residue	Details
A	GLU475

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10095

Chain	Residue	Details
A	HIS474
A	HIS478
A	HIS481

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q9BYT8

Chain	Residue	Details
A	LYS641

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1i1i

Chain	Residue	Details
A	TYR613
A	GLU503

---

site_id	MCSA1
Number of Residues	5
Details	M-CSA 638

Chain	Residue	Details
A	HIS474
A	GLU475
A	HIS478
A	GLU503	proton shuttle (general acid/base)
A	TYR613	electrostatic stabiliser

---