2O3E
Crystal structure of engineered neurolysin with thimet oligopeptidase specificity for neurotensin cleavage site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 159.550, 87.700, 58.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.850 - 2.200 |
| Rwork | 0.219 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i1i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.088 | 0.412 |
| Number of reflections | 42607 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
