Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2IK8

Crystal structure of the heterodimeric complex of human RGS16 and activated Gi alpha 1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001664	molecular_function	G protein-coupled receptor binding
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005794	cellular_component	Golgi apparatus
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005834	cellular_component	heterotrimeric G-protein complex
A	0005856	cellular_component	cytoskeleton
A	0005886	cellular_component	plasma membrane
A	0005938	cellular_component	cell cortex
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0007198	biological_process	adenylate cyclase-inhibiting serotonin receptor signaling pathway
A	0007218	biological_process	neuropeptide signaling pathway
A	0010854	molecular_function	adenylate cyclase regulator activity
A	0010855	molecular_function	adenylate cyclase inhibitor activity
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0019001	molecular_function	guanyl nucleotide binding
A	0019003	molecular_function	GDP binding
A	0030496	cellular_component	midbody
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
A	0031749	molecular_function	D2 dopamine receptor binding
A	0031821	molecular_function	G protein-coupled serotonin receptor binding
A	0034451	cellular_component	centriolar satellite
A	0034695	biological_process	response to prostaglandin E
A	0036064	cellular_component	ciliary basal body
A	0043434	biological_process	response to peptide hormone
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0046676	biological_process	negative regulation of insulin secretion
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0060236	biological_process	regulation of mitotic spindle organization
A	0070062	cellular_component	extracellular exosome
A	0070098	biological_process	chemokine-mediated signaling pathway
A	0072678	biological_process	T cell migration
A	1901082	biological_process	positive regulation of relaxation of smooth muscle
A	1904322	biological_process	cellular response to forskolin
A	1904778	biological_process	positive regulation of protein localization to cell cortex
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0001664	molecular_function	G protein-coupled receptor binding
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005794	cellular_component	Golgi apparatus
C	0005813	cellular_component	centrosome
C	0005829	cellular_component	cytosol
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005856	cellular_component	cytoskeleton
C	0005886	cellular_component	plasma membrane
C	0005938	cellular_component	cell cortex
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
C	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
C	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
C	0007198	biological_process	adenylate cyclase-inhibiting serotonin receptor signaling pathway
C	0007218	biological_process	neuropeptide signaling pathway
C	0010854	molecular_function	adenylate cyclase regulator activity
C	0010855	molecular_function	adenylate cyclase inhibitor activity
C	0016020	cellular_component	membrane
C	0016787	molecular_function	hydrolase activity
C	0019001	molecular_function	guanyl nucleotide binding
C	0019003	molecular_function	GDP binding
C	0030496	cellular_component	midbody
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0031749	molecular_function	D2 dopamine receptor binding
C	0031821	molecular_function	G protein-coupled serotonin receptor binding
C	0034451	cellular_component	centriolar satellite
C	0034695	biological_process	response to prostaglandin E
C	0036064	cellular_component	ciliary basal body
C	0043434	biological_process	response to peptide hormone
C	0045542	biological_process	positive regulation of cholesterol biosynthetic process
C	0046676	biological_process	negative regulation of insulin secretion
C	0046872	molecular_function	metal ion binding
C	0051301	biological_process	cell division
C	0060236	biological_process	regulation of mitotic spindle organization
C	0070062	cellular_component	extracellular exosome
C	0070098	biological_process	chemokine-mediated signaling pathway
C	0072678	biological_process	T cell migration
C	1901082	biological_process	positive regulation of relaxation of smooth muscle
C	1904322	biological_process	cellular response to forskolin
C	1904778	biological_process	positive regulation of protein localization to cell cortex

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ALF A 355

Chain	Residue
A	GLY42
A	MG356
A	GDP401
A	HOH408
A	HOH416
A	HOH417
A	GLU43
A	LYS46
A	ARG178
A	LYS180
A	THR181
A	VAL201
A	GLY203
A	GLN204

site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ALF C 355

Chain	Residue
C	GLY42
C	GLU43
C	LYS46
C	SER47
C	ARG178
C	VAL179
C	LYS180
C	THR181
C	GLY203
C	GLN204
C	MG356
C	GDP402
C	HOH405
C	HOH407
C	HOH413

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 356

Chain	Residue
A	SER47
A	THR181
A	ALF355
A	GDP401
A	HOH408
A	HOH417

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 356

Chain	Residue
C	SER47
C	THR181
C	ALF355
C	GDP402
C	HOH405
C	HOH413

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 357

Chain	Residue
A	ILE93
A	ASP94
C	GLU297

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 302

Chain	Residue
B	ILE104
B	ARG105
B	SER106

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 303

Chain	Residue
D	LYS103
D	ILE104
D	ARG105
D	SER106

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 357

Chain	Residue
A	ARG105
C	HIS322
C	PHE323

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 358

Chain	Residue
A	SER293
A	ASN294
C	ARG90

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 359

Chain	Residue
A	LYS35
A	PHE199

site_id	BC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 358

Chain	Residue
C	GLN106

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 360

Chain	Residue
A	HIS213
C	GLY217

site_id	BC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE GDP A 401

Chain	Residue
A	GLU43
A	SER44
A	GLY45
A	LYS46
A	SER47
A	THR48
A	SER151
A	LEU175
A	ARG176
A	THR177
A	ARG178
A	ASN269
A	LYS270
A	ASP272
A	CYS325
A	ALA326
A	THR327
A	ALF355
A	MG356
A	HOH405
A	HOH408
A	HOH417

site_id	BC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE GDP C 402

Chain	Residue
C	LEU273
C	CYS325
C	ALA326
C	THR327
C	ALF355
C	MG356
C	HOH405
C	HOH413
C	GLU43
C	SER44
C	GLY45
C	LYS46
C	SER47
C	THR48
C	ASP150
C	SER151
C	LEU175
C	ARG176
C	THR177
C	ARG178
C	ASN269
C	LYS270
C	ASP272

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphotyrosine; by EGFR => ECO:0000269\|PubMed:11602604

Chain	Residue	Details
B	TYR168
D	TYR168
C	SER44
C	LYS270

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000269\|PubMed:11602604

Chain	Residue	Details
B	TYR177
D	TYR177
C	THR48
C	THR182

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744\|PDB:1KJY, ECO:0007744\|PDB:2OM2, ECO:0007744\|PDB:2XNS, ECO:0007744\|PDB:3ONW, ECO:0007744\|PDB:3QE0, ECO:0007744\|PDB:3QI2, ECO:0007744\|PDB:4G5Q

Chain	Residue	Details
A	ALA152
C	ALA152

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744\|PDB:1KJY, ECO:0007744\|PDB:1Y3A, ECO:0007744\|PDB:2G83, ECO:0007744\|PDB:2GTP, ECO:0007744\|PDB:2IK8, ECO:0007744\|PDB:2OM2, ECO:0007744\|PDB:2XNS, ECO:0007744\|PDB:3ONW, ECO:0007744\|PDB:3QE0, ECO:0007744\|PDB:3QI2, ECO:0007744\|PDB:3UMR, ECO:0007744\|PDB:3UMS, ECO:0007744\|PDB:4G5Q

Chain	Residue	Details
A	ARG176
C	ARG176

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:21115486

Chain	Residue	Details
A	VAL201
C	VAL201

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744\|PDB:1Y3A, ECO:0007744\|PDB:2G83, ECO:0007744\|PDB:2GTP, ECO:0007744\|PDB:2IK8, ECO:0007744\|PDB:2OM2, ECO:0007744\|PDB:3ONW, ECO:0007744\|PDB:3QE0, ECO:0007744\|PDB:3QI2, ECO:0007744\|PDB:3UMR, ECO:0007744\|PDB:3UMS, ECO:0007744\|PDB:4G5Q

Chain	Residue	Details
A	THR327
C	THR327

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250

Chain	Residue	Details
A	VAL179
C	VAL179

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Deamidated glutamine; by Photorhabdus PAU_02230 => ECO:0000269\|PubMed:24141704

Chain	Residue	Details
A	ARG205
C	ARG205

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: ADP-ribosylcysteine; by pertussis toxin => ECO:0000250

Chain	Residue	Details
A	GLY352
C	GLY352

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	THR181
A	GLN204
A	GLU43
A	ARG178

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	THR181
C	GLN204
C	GLU43
C	ARG178

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	GLN204

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	GLN204

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)