2IK8
Crystal structure of the heterodimeric complex of human RGS16 and activated Gi alpha 1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001664 | molecular_function | G protein-coupled receptor binding |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005730 | cellular_component | nucleolus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005813 | cellular_component | centrosome |
| A | 0005834 | cellular_component | heterotrimeric G-protein complex |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005938 | cellular_component | cell cortex |
| A | 0007165 | biological_process | signal transduction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| A | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| A | 0016020 | cellular_component | membrane |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0019003 | molecular_function | GDP binding |
| A | 0030496 | cellular_component | midbody |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| A | 0031749 | molecular_function | D2 dopamine receptor binding |
| A | 0031821 | molecular_function | G protein-coupled serotonin receptor binding |
| A | 0043434 | biological_process | response to peptide hormone |
| A | 0043949 | biological_process | regulation of cAMP-mediated signaling |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051301 | biological_process | cell division |
| A | 0060236 | biological_process | regulation of mitotic spindle organization |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0072678 | biological_process | T cell migration |
| A | 1904322 | biological_process | cellular response to forskolin |
| A | 1904778 | biological_process | positive regulation of protein localization to cell cortex |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0001664 | molecular_function | G protein-coupled receptor binding |
| C | 0003924 | molecular_function | GTPase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005525 | molecular_function | GTP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005730 | cellular_component | nucleolus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005765 | cellular_component | lysosomal membrane |
| C | 0005813 | cellular_component | centrosome |
| C | 0005834 | cellular_component | heterotrimeric G-protein complex |
| C | 0005856 | cellular_component | cytoskeleton |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0005938 | cellular_component | cell cortex |
| C | 0007165 | biological_process | signal transduction |
| C | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| C | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| C | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| C | 0016020 | cellular_component | membrane |
| C | 0019001 | molecular_function | guanyl nucleotide binding |
| C | 0019003 | molecular_function | GDP binding |
| C | 0030496 | cellular_component | midbody |
| C | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| C | 0031749 | molecular_function | D2 dopamine receptor binding |
| C | 0031821 | molecular_function | G protein-coupled serotonin receptor binding |
| C | 0043434 | biological_process | response to peptide hormone |
| C | 0043949 | biological_process | regulation of cAMP-mediated signaling |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051301 | biological_process | cell division |
| C | 0060236 | biological_process | regulation of mitotic spindle organization |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0072678 | biological_process | T cell migration |
| C | 1904322 | biological_process | cellular response to forskolin |
| C | 1904778 | biological_process | positive regulation of protein localization to cell cortex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE ALF A 355 |
| Chain | Residue |
| A | GLY42 |
| A | MG356 |
| A | GDP401 |
| A | HOH408 |
| A | HOH416 |
| A | HOH417 |
| A | GLU43 |
| A | LYS46 |
| A | ARG178 |
| A | LYS180 |
| A | THR181 |
| A | VAL201 |
| A | GLY203 |
| A | GLN204 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE ALF C 355 |
| Chain | Residue |
| C | GLY42 |
| C | GLU43 |
| C | LYS46 |
| C | SER47 |
| C | ARG178 |
| C | VAL179 |
| C | LYS180 |
| C | THR181 |
| C | GLY203 |
| C | GLN204 |
| C | MG356 |
| C | GDP402 |
| C | HOH405 |
| C | HOH407 |
| C | HOH413 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 356 |
| Chain | Residue |
| A | SER47 |
| A | THR181 |
| A | ALF355 |
| A | GDP401 |
| A | HOH408 |
| A | HOH417 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG C 356 |
| Chain | Residue |
| C | SER47 |
| C | THR181 |
| C | ALF355 |
| C | GDP402 |
| C | HOH405 |
| C | HOH413 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 357 |
| Chain | Residue |
| A | ILE93 |
| A | ASP94 |
| C | GLU297 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 302 |
| Chain | Residue |
| B | ILE104 |
| B | ARG105 |
| B | SER106 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 303 |
| Chain | Residue |
| D | LYS103 |
| D | ILE104 |
| D | ARG105 |
| D | SER106 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 357 |
| Chain | Residue |
| A | ARG105 |
| C | HIS322 |
| C | PHE323 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 358 |
| Chain | Residue |
| A | SER293 |
| A | ASN294 |
| C | ARG90 |
| site_id | BC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 359 |
| Chain | Residue |
| A | LYS35 |
| A | PHE199 |
| site_id | BC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 C 358 |
| Chain | Residue |
| C | GLN106 |
| site_id | BC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 360 |
| Chain | Residue |
| A | HIS213 |
| C | GLY217 |
| site_id | BC4 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE GDP A 401 |
| Chain | Residue |
| A | GLU43 |
| A | SER44 |
| A | GLY45 |
| A | LYS46 |
| A | SER47 |
| A | THR48 |
| A | SER151 |
| A | LEU175 |
| A | ARG176 |
| A | THR177 |
| A | ARG178 |
| A | ASN269 |
| A | LYS270 |
| A | ASP272 |
| A | CYS325 |
| A | ALA326 |
| A | THR327 |
| A | ALF355 |
| A | MG356 |
| A | HOH405 |
| A | HOH408 |
| A | HOH417 |
| site_id | BC5 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE GDP C 402 |
| Chain | Residue |
| C | LEU273 |
| C | CYS325 |
| C | ALA326 |
| C | THR327 |
| C | ALF355 |
| C | MG356 |
| C | HOH405 |
| C | HOH413 |
| C | GLU43 |
| C | SER44 |
| C | GLY45 |
| C | LYS46 |
| C | SER47 |
| C | THR48 |
| C | ASP150 |
| C | SER151 |
| C | LEU175 |
| C | ARG176 |
| C | THR177 |
| C | ARG178 |
| C | ASN269 |
| C | LYS270 |
| C | ASP272 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by EGFR => ECO:0000269|PubMed:11602604 |
| Chain | Residue | Details |
| B | TYR168 | |
| D | TYR168 | |
| C | SER44 | |
| C | LYS270 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:11602604 |
| Chain | Residue | Details |
| B | TYR177 | |
| D | TYR177 | |
| C | THR48 | |
| C | THR182 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | ALA152 | |
| C | ALA152 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | ARG176 | |
| C | ARG176 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:21115486 |
| Chain | Residue | Details |
| A | VAL201 | |
| C | VAL201 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | THR327 | |
| C | THR327 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250 |
| Chain | Residue | Details |
| A | VAL179 | |
| C | VAL179 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | MOD_RES: Deamidated glutamine; by Photorhabdus PAU_02230 => ECO:0000269|PubMed:24141704 |
| Chain | Residue | Details |
| A | ARG205 | |
| C | ARG205 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylcysteine; by pertussis toxin => ECO:0000250 |
| Chain | Residue | Details |
| A | GLY352 | |
| C | GLY352 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| A | THR181 | |
| A | GLN204 | |
| A | GLU43 | |
| A | ARG178 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| C | THR181 | |
| C | GLN204 | |
| C | GLU43 | |
| C | ARG178 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| A | GLN204 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| C | GLN204 |
