2IK8

Crystal structure of the heterodimeric complex of human RGS16 and activated Gi alpha 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9198
Spacegroup name	P 21 21 21
Unit cell lengths	82.656, 104.312, 124.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.600 - 2.710
R-factor	0.23544
Rwork	0.233
R-free	0.29106
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID = 2GTP
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	27900
Completeness [%]	99.3	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2M (NH4)2SO4, 0.1M Bis-Tris, pH 5.5, 25% PEG3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K