2HA7
Crystal structure of mutant S203A of mouse acetylcholinesterase complexed with butyrylthiocholine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001919 | biological_process | regulation of receptor recycling |
| A | 0002076 | biological_process | osteoblast development |
| A | 0003990 | molecular_function | acetylcholinesterase activity |
| A | 0004104 | molecular_function | cholinesterase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005518 | molecular_function | collagen binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005604 | cellular_component | basement membrane |
| A | 0005615 | cellular_component | extracellular space |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006581 | biological_process | acetylcholine catabolic process |
| A | 0007155 | biological_process | cell adhesion |
| A | 0009986 | cellular_component | cell surface |
| A | 0016020 | cellular_component | membrane |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0017171 | molecular_function | serine hydrolase activity |
| A | 0031594 | cellular_component | neuromuscular junction |
| A | 0031623 | biological_process | receptor internalization |
| A | 0042166 | molecular_function | acetylcholine binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0043236 | molecular_function | laminin binding |
| A | 0045202 | cellular_component | synapse |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| A | 0060041 | biological_process | retina development in camera-type eye |
| A | 0095500 | biological_process | acetylcholine receptor signaling pathway |
| A | 0098552 | cellular_component | side of membrane |
| A | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
| B | 0001919 | biological_process | regulation of receptor recycling |
| B | 0002076 | biological_process | osteoblast development |
| B | 0003990 | molecular_function | acetylcholinesterase activity |
| B | 0004104 | molecular_function | cholinesterase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005518 | molecular_function | collagen binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005604 | cellular_component | basement membrane |
| B | 0005615 | cellular_component | extracellular space |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006581 | biological_process | acetylcholine catabolic process |
| B | 0007155 | biological_process | cell adhesion |
| B | 0009986 | cellular_component | cell surface |
| B | 0016020 | cellular_component | membrane |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0017171 | molecular_function | serine hydrolase activity |
| B | 0031594 | cellular_component | neuromuscular junction |
| B | 0031623 | biological_process | receptor internalization |
| B | 0042166 | molecular_function | acetylcholine binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0043236 | molecular_function | laminin binding |
| B | 0045202 | cellular_component | synapse |
| B | 0048471 | cellular_component | perinuclear region of cytoplasm |
| B | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| B | 0060041 | biological_process | retina development in camera-type eye |
| B | 0095500 | biological_process | acetylcholine receptor signaling pathway |
| B | 0098552 | cellular_component | side of membrane |
| B | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IOD A 900 |
| Chain | Residue |
| A | PHE295 |
| A | BCH910 |
| site_id | AC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD A 902 |
| Chain | Residue |
| A | ARG245 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE IOD A 903 |
| Chain | Residue |
| A | PRO290 |
| A | GLN291 |
| A | SER293 |
| site_id | AC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD B 904 |
| Chain | Residue |
| B | ARG245 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD B 905 |
| Chain | Residue |
| B | SER293 |
| site_id | AC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD B 906 |
| Chain | Residue |
| B | LYS332 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IOD A 907 |
| Chain | Residue |
| A | VAL255 |
| A | ASP280 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IOD B 908 |
| Chain | Residue |
| B | LYS53 |
| B | HOH966 |
| site_id | AC9 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD B 909 |
| Chain | Residue |
| B | PHE295 |
| site_id | BC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE ETM A 908 |
| Chain | Residue |
| A | TRP86 |
| A | GLY120 |
| A | GLY121 |
| A | GLU202 |
| A | TYR337 |
| A | HIS447 |
| A | BUA909 |
| A | HOH1001 |
| site_id | BC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE BUA A 909 |
| Chain | Residue |
| A | GLY121 |
| A | GLY122 |
| A | TYR124 |
| A | ALA203 |
| A | ALA204 |
| A | HIS447 |
| A | ETM908 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE BCH A 910 |
| Chain | Residue |
| A | TYR72 |
| A | TYR124 |
| A | TRP286 |
| A | TYR337 |
| A | TYR341 |
| A | IOD900 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE ETM B 602 |
| Chain | Residue |
| B | TRP86 |
| B | GLY120 |
| B | GLY121 |
| B | GLU202 |
| B | TYR337 |
| B | HIS447 |
| B | BUA603 |
| site_id | BC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE BUA B 603 |
| Chain | Residue |
| B | GLY121 |
| B | GLY122 |
| B | TYR124 |
| B | ALA203 |
| B | ALA204 |
| B | HIS447 |
| B | ETM602 |
| site_id | BC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE BCH A 601 |
| Chain | Residue |
| A | ASP396 |
| A | TRP442 |
| site_id | BC7 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE P6G A 901 |
| Chain | Residue |
| A | LEU380 |
| A | HIS381 |
| A | GLN527 |
| A | PHE535 |
| A | HOH913 |
| B | ALA377 |
| B | LEU380 |
| B | HIS381 |
| B | PHE531 |
| B | PHE535 |
Functional Information from PROSITE/UniProt
| site_id | PS00941 |
| Number of Residues | 11 |
| Details | CARBOXYLESTERASE_B_2 Carboxylesterases type-B signature 2. EDCLYLNVWtP |
| Chain | Residue | Details |
| A | GLU94-PRO104 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Acyl-ester intermediate |
| Chain | Residue | Details |
| A | ALA203 | |
| B | ALA203 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Charge relay system |
| Chain | Residue | Details |
| A | GLU334 | |
| A | HIS447 | |
| B | GLU334 | |
| B | HIS447 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN265 | |
| B | ASN265 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine |
| Chain | Residue | Details |
| A | ASN350 | |
| A | ASN464 | |
| B | ASN350 | |
| B | ASN464 |
