2DHF

CRYSTAL STRUCTURES OF RECOMBINANT HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 5-DEAZOFOLATE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0004146	molecular_function	dihydrofolate reductase activity
A	0005515	molecular_function	protein binding
A	0005542	molecular_function	folic acid binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006729	biological_process	tetrahydrobiopterin biosynthetic process
A	0006730	biological_process	one-carbon metabolic process
A	0008144	molecular_function	obsolete drug binding
A	0016491	molecular_function	oxidoreductase activity
A	0017148	biological_process	negative regulation of translation
A	0031103	biological_process	axon regeneration
A	0031427	biological_process	response to methotrexate
A	0046452	biological_process	dihydrofolate metabolic process
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0046654	biological_process	tetrahydrofolate biosynthetic process
A	0046655	biological_process	folic acid metabolic process
A	0050661	molecular_function	NADP binding
A	0060090	molecular_function	molecular adaptor activity
A	0070402	molecular_function	NADPH binding
A	1990825	molecular_function	sequence-specific mRNA binding
A	2000121	biological_process	regulation of removal of superoxide radicals
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0003723	molecular_function	RNA binding
B	0003729	molecular_function	mRNA binding
B	0004146	molecular_function	dihydrofolate reductase activity
B	0005515	molecular_function	protein binding
B	0005542	molecular_function	folic acid binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006729	biological_process	tetrahydrobiopterin biosynthetic process
B	0006730	biological_process	one-carbon metabolic process
B	0008144	molecular_function	obsolete drug binding
B	0016491	molecular_function	oxidoreductase activity
B	0017148	biological_process	negative regulation of translation
B	0031103	biological_process	axon regeneration
B	0031427	biological_process	response to methotrexate
B	0046452	biological_process	dihydrofolate metabolic process
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0046654	biological_process	tetrahydrofolate biosynthetic process
B	0046655	biological_process	folic acid metabolic process
B	0050661	molecular_function	NADP binding
B	0060090	molecular_function	molecular adaptor activity
B	0070402	molecular_function	NADPH binding
B	1990825	molecular_function	sequence-specific mRNA binding
B	2000121	biological_process	regulation of removal of superoxide radicals

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE DZF A 187

Chain	Residue
A	ILE7
A	ILE60
A	PRO61
A	LEU67
A	ARG70
A	VAL115
A	TYR121
A	THR136
A	HOH403
A	VAL8
A	ALA9
A	LEU22
A	GLU30
A	PHE31
A	PHE34
A	GLN35
A	THR56

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site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE DZF B 187

Chain	Residue
B	ILE7
B	VAL8
B	GLU30
B	PHE31
B	PHE34
B	GLN35
B	ASN64
B	ARG70
B	VAL115
B	TYR121
B	THR136
B	HOH401
B	HOH410
B	HOH431

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Functional Information from PROSITE/UniProt

site_id	PS00075
Number of Residues	24
Details	DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGkngdLPWpplrnEfryFqrmT

Chain	Residue	Details
A	GLY15-THR38

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15039552","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16222560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19478082","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"2248959","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
A	LEU22
A	GLU30

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site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
B	LEU22
B	GLU30

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site_id	MCSA1
Number of Residues	2
Details	M-CSA 490

Chain	Residue	Details
A	LEU22	electrostatic stabiliser
A	GLU30	electrostatic stabiliser

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site_id	MCSA2
Number of Residues	2
Details	M-CSA 490

Chain	Residue	Details
B	LEU22	electrostatic stabiliser
B	GLU30	electrostatic stabiliser

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