2DHF

CRYSTAL STRUCTURES OF RECOMBINANT HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 5-DEAZOFOLATE

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	36.100, 38.500, 76.800
Unit cell angles	93.90, 91.40, 111.50

Refinement procedure

Resolution	? - 2.300
R-factor	0.194
RMSD bond length	0.021
RMSD bond angle	0.035
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.050 *
Number of reflections	15545 *
Completeness [%]	95.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	0.025 (M)
2	1	drop	dithioerythritol	1 (mM)
3	1	drop	enzyme	10 (mg/ml)
4	1	drop	folate		3-fold molar ecess of substrate to enzyme
5	1	reservoir	sodium acetate	0.17 (M)
6	1	reservoir	PEG4000	20 (%(w/v))
7	1	reservoir	ethanol	7 (%(v/v))