1Y8Q
SUMO E1 ACTIVATING ENZYME SAE1-SAE2-MG-ATP COMPLEX
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004839 | molecular_function | ubiquitin activating enzyme activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0008047 | molecular_function | enzyme activator activity |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016874 | molecular_function | ligase activity |
| A | 0016925 | biological_process | protein sumoylation |
| A | 0019948 | molecular_function | SUMO activating enzyme activity |
| A | 0031510 | cellular_component | SUMO activating enzyme complex |
| A | 0033235 | biological_process | positive regulation of protein sumoylation |
| A | 0036211 | biological_process | protein modification process |
| A | 0043008 | molecular_function | ATP-dependent protein binding |
| A | 0044388 | molecular_function | small protein activating enzyme binding |
| A | 0046982 | molecular_function | protein heterodimerization activity |
| A | 1903955 | biological_process | obsolete positive regulation of protein targeting to mitochondrion |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016925 | biological_process | protein sumoylation |
| B | 0019948 | molecular_function | SUMO activating enzyme activity |
| B | 0031510 | cellular_component | SUMO activating enzyme complex |
| B | 0032183 | molecular_function | SUMO binding |
| B | 0033235 | biological_process | positive regulation of protein sumoylation |
| B | 0044388 | molecular_function | small protein activating enzyme binding |
| B | 0044390 | molecular_function | ubiquitin-like protein conjugating enzyme binding |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0046982 | molecular_function | protein heterodimerization activity |
| C | 0004839 | molecular_function | ubiquitin activating enzyme activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0008047 | molecular_function | enzyme activator activity |
| C | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| C | 0016567 | biological_process | protein ubiquitination |
| C | 0016874 | molecular_function | ligase activity |
| C | 0016925 | biological_process | protein sumoylation |
| C | 0019948 | molecular_function | SUMO activating enzyme activity |
| C | 0031510 | cellular_component | SUMO activating enzyme complex |
| C | 0033235 | biological_process | positive regulation of protein sumoylation |
| C | 0036211 | biological_process | protein modification process |
| C | 0043008 | molecular_function | ATP-dependent protein binding |
| C | 0044388 | molecular_function | small protein activating enzyme binding |
| C | 0046982 | molecular_function | protein heterodimerization activity |
| C | 1903955 | biological_process | obsolete positive regulation of protein targeting to mitochondrion |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005654 | cellular_component | nucleoplasm |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016925 | biological_process | protein sumoylation |
| D | 0019948 | molecular_function | SUMO activating enzyme activity |
| D | 0031510 | cellular_component | SUMO activating enzyme complex |
| D | 0032183 | molecular_function | SUMO binding |
| D | 0033235 | biological_process | positive regulation of protein sumoylation |
| D | 0044388 | molecular_function | small protein activating enzyme binding |
| D | 0044390 | molecular_function | ubiquitin-like protein conjugating enzyme binding |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0046982 | molecular_function | protein heterodimerization activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 641 |
| Chain | Residue |
| D | ASP117 |
| D | ATP801 |
| D | HOH851 |
| D | HOH903 |
| D | HOH991 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 641 |
| Chain | Residue |
| B | HOH1065 |
| B | ASP117 |
| B | ATP802 |
| B | HOH935 |
| B | HOH978 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN D 642 |
| Chain | Residue |
| D | CYS158 |
| D | CYS161 |
| D | CYS441 |
| D | CYS444 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 642 |
| Chain | Residue |
| B | CYS158 |
| B | CYS161 |
| B | CYS441 |
| B | CYS444 |
| site_id | AC5 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE ATP D 801 |
| Chain | Residue |
| C | ARG21 |
| D | GLY26 |
| D | GLY27 |
| D | ASP48 |
| D | LEU49 |
| D | ASP50 |
| D | ASN56 |
| D | ARG59 |
| D | GLN60 |
| D | LYS72 |
| D | ASP94 |
| D | SER95 |
| D | ILE96 |
| D | LEU116 |
| D | ASP117 |
| D | LYS346 |
| D | MG641 |
| D | HOH837 |
| D | HOH842 |
| D | HOH851 |
| D | HOH866 |
| D | HOH903 |
| D | HOH908 |
| D | HOH991 |
| site_id | AC6 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE ATP B 802 |
| Chain | Residue |
| A | ARG21 |
| B | GLY26 |
| B | GLY27 |
| B | ASP48 |
| B | LEU49 |
| B | ASP50 |
| B | ASN56 |
| B | ARG59 |
| B | GLN60 |
| B | LYS72 |
| B | ASP94 |
| B | SER95 |
| B | ILE96 |
| B | MET97 |
| B | LEU116 |
| B | ASP117 |
| B | ASN118 |
| B | ALA121 |
| B | LYS346 |
| B | MG641 |
| B | HOH818 |
| B | HOH829 |
| B | HOH839 |
| B | HOH875 |
| B | HOH879 |
| B | HOH918 |
| B | HOH935 |
| B | HOH978 |
| B | HOH1065 |
Functional Information from PROSITE/UniProt
| site_id | PS00536 |
| Number of Residues | 9 |
| Details | UBIQUITIN_ACTIVAT_1 Ubiquitin-activating enzyme signature 1. KILSGKIdQ |
| Chain | Residue | Details |
| B | LYS404-GLN412 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10132","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15660128","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20164921","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 32 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15660128","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)","evidences":[{"source":"PubMed","id":"25114211","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate"} |
| Chain | Residue | Details |
