1Y8Q
SUMO E1 ACTIVATING ENZYME SAE1-SAE2-MG-ATP COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.245, 116.667, 106.078 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.250 |
| R-factor | 0.2081 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 | 0.435 |
| Number of reflections | 108617 | |
| <I/σ(I)> | 16.4 | 2.6 |
| Completeness [%] | 97.3 | 92.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 278 | 0.1M sodium acetate, 10mM ATP, 10-20% PEG4000, 0.1-1.0M ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
