1SCN

INACTIVATION OF SUBTILISIN CARLSBERG BY N-(TERT-BUTOXYCARBONYL-ALANYL-PROLYL-PHENYLALANYL)-O-BENZOL HYDROXYLAMINE: FORMATION OF COVALENT ENZYME-INHIBITOR LINKAGE IN THE FORM OF A CARBAMATE DERIVATIVE

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0004252	molecular_function	serine-type endopeptidase activity
E	0006508	biological_process	proteolysis
E	0008236	molecular_function	serine-type peptidase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE 0EF E 447

Chain	Residue
E	SER99
E	GLN245
E	ARG249
E	HOH650
E	HOH659
E	HOH662
E	HOH802
E	HOH850
E	HOH852
E	HOH861
E	GLY100
E	LEU126
E	GLY127
E	GLY128
E	ALA152
E	GLY154
E	ASN155
E	SER221

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 401

Chain	Residue
E	GLN2
E	ASP41
E	LEU75
E	ASN77
E	THR79
E	VAL81

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA E 402

Chain	Residue
E	VAL174
E	ALA176
E	GLU195
E	GLU197

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA E 403

Chain	Residue
E	ALA37
E	SER38
E	HIS39
E	LEU42

---

site_id	ACT
Number of Residues	3
Details	CATALYTIC TRIAD IN THE ENZYME ACTIVE SITE

Chain	Residue
E	ASP32
E	HIS64
E	SER221

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Functional Information from PROSITE/UniProt

site_id	PS00136
Number of Residues	12
Details	SUBTILASE_ASP Serine proteases, subtilase family, aspartic acid active site. VAVLDTGIqasH

Chain	Residue	Details
E	VAL28-HIS39

---

site_id	PS00137
Number of Residues	11
Details	SUBTILASE_HIS Serine proteases, subtilase family, histidine active site. HGThVAGtVAA

Chain	Residue	Details
E	HIS64-ALA74

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site_id	PS00138
Number of Residues	11
Details	SUBTILASE_SER Serine proteases, subtilase family, serine active site. GTSmAsPhVAG

Chain	Residue	Details
E	GLY219-GLY229

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Charge relay system => ECO:0000255|PROSITE-ProRule:PRU01240

Chain	Residue	Details
E	ASP32
E	HIS64
E	SER221

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	BINDING: BINDING => ECO:0000269|PubMed:8512925

Chain	Residue	Details
E	GLN2
E	ASP41
E	LEU75
E	ASN77
E	THR79
E	VAL81
E	ALA169
E	TYR171
E	VAL174

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