1R8Q
FULL-LENGTH ARF1-GDP-MG IN COMPLEX WITH BREFELDIN A AND A SEC7 DOMAIN
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000139 | cellular_component | Golgi membrane |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0002090 | biological_process | regulation of receptor internalization |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0003925 | molecular_function | G protein activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006886 | biological_process | intracellular protein transport |
| A | 0014069 | cellular_component | postsynaptic density |
| A | 0015031 | biological_process | protein transport |
| A | 0016192 | biological_process | vesicle-mediated transport |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0034315 | biological_process | regulation of Arp2/3 complex-mediated actin nucleation |
| A | 0042583 | cellular_component | chromaffin granule |
| A | 0043005 | cellular_component | neuron projection |
| A | 0045202 | cellular_component | synapse |
| A | 0060292 | biological_process | long-term synaptic depression |
| A | 0097061 | biological_process | dendritic spine organization |
| B | 0000139 | cellular_component | Golgi membrane |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0002090 | biological_process | regulation of receptor internalization |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0003925 | molecular_function | G protein activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005525 | molecular_function | GTP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006886 | biological_process | intracellular protein transport |
| B | 0014069 | cellular_component | postsynaptic density |
| B | 0015031 | biological_process | protein transport |
| B | 0016192 | biological_process | vesicle-mediated transport |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0034315 | biological_process | regulation of Arp2/3 complex-mediated actin nucleation |
| B | 0042583 | cellular_component | chromaffin granule |
| B | 0043005 | cellular_component | neuron projection |
| B | 0045202 | cellular_component | synapse |
| B | 0060292 | biological_process | long-term synaptic depression |
| B | 0097061 | biological_process | dendritic spine organization |
| E | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| E | 0032012 | biological_process | regulation of ARF protein signal transduction |
| F | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| F | 0032012 | biological_process | regulation of ARF protein signal transduction |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 402 |
| Chain | Residue |
| A | THR31 |
| A | G3D401 |
| A | HOH410 |
| A | HOH450 |
| A | HOH504 |
| A | HOH510 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 502 |
| Chain | Residue |
| B | HOH563 |
| B | HOH567 |
| B | HOH570 |
| B | THR31 |
| B | G3D501 |
| B | HOH517 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN E 601 |
| Chain | Residue |
| E | HIS127 |
| E | ASP131 |
| F | HIS127 |
| F | ASP131 |
| site_id | AC4 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE G3D A 401 |
| Chain | Residue |
| A | ALA27 |
| A | ALA28 |
| A | GLY29 |
| A | LYS30 |
| A | THR31 |
| A | THR32 |
| A | ASN126 |
| A | LYS127 |
| A | ASP129 |
| A | LEU130 |
| A | CYS159 |
| A | ALA160 |
| A | THR161 |
| A | MG402 |
| A | HOH410 |
| A | HOH432 |
| A | HOH435 |
| A | HOH445 |
| A | HOH504 |
| A | HOH510 |
| B | LYS142 |
| site_id | AC5 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE AFB A 403 |
| Chain | Residue |
| A | VAL53 |
| A | THR64 |
| A | VAL65 |
| A | TRP66 |
| A | ASP67 |
| A | TRP78 |
| A | TYR81 |
| A | HOH406 |
| A | HOH414 |
| E | TYR190 |
| E | MET194 |
| site_id | AC6 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE G3D B 501 |
| Chain | Residue |
| A | LYS142 |
| B | ALA27 |
| B | ALA28 |
| B | GLY29 |
| B | LYS30 |
| B | THR31 |
| B | THR32 |
| B | ASN126 |
| B | LYS127 |
| B | ASP129 |
| B | LEU130 |
| B | CYS159 |
| B | ALA160 |
| B | THR161 |
| B | MG502 |
| B | HOH563 |
| B | HOH567 |
| B | HOH569 |
| B | HOH570 |
| site_id | AC7 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE AFB B 503 |
| Chain | Residue |
| B | VAL53 |
| B | THR64 |
| B | VAL65 |
| B | TRP66 |
| B | ASP67 |
| B | TRP78 |
| B | TYR81 |
| B | HOH506 |
| B | HOH508 |
| B | HOH513 |
| F | TYR190 |
| F | MET194 |
| F | THR197 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 26 |
| Details | Region: {"description":"Important for the stable binding to the membranes","evidences":[{"source":"PubMed","id":"9109679","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"14654833","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24058294","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1R8Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1R8S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1S9D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4C0A","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N-acetylglycine; alternate","evidences":[{"source":"UniProtKB","id":"P84077","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Lipidation: {"description":"N-myristoyl glycine; alternate","evidences":[{"source":"UniProtKB","id":"P84077","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 258 |
| Details | Domain: {"description":"SEC7","evidences":[{"source":"PROSITE-ProRule","id":"PRU00189","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| A | GLN71 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| B | GLN71 |
