1R8Q
FULL-LENGTH ARF1-GDP-MG IN COMPLEX WITH BREFELDIN A AND A SEC7 DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Wavelength(s) | 0.931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.081, 109.773, 118.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 1.860 |
R-factor | 0.18111 |
Rwork | 0.179 |
R-free | 0.22200 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 1.971 |
Data scaling software | XDS |
Phasing software | CCP4 ((MOLREP)) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.880 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 74905 | |
<I/σ(I)> | 18.6 | 3.1 |
Completeness [%] | 83.6 | |
Redundancy | 11.77 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 16 * | 19% Peg 3350, 175mM NH4 Fluoride, 100mM cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 19 (%) | |
2 | 1 | reservoir | ammonium fluoride | 175 (mM) | |
3 | 1 | reservoir | cacodylate | 100 (mM) | pH6.5 |
4 | 1 | reservoir | sucrose | 17 (%) |