1R1I
STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001786 | molecular_function | phosphatidylserine binding |
| A | 0001822 | biological_process | kidney development |
| A | 0001890 | biological_process | placenta development |
| A | 0002003 | biological_process | angiotensin maturation |
| A | 0004175 | molecular_function | endopeptidase activity |
| A | 0004181 | molecular_function | metallocarboxypeptidase activity |
| A | 0004222 | molecular_function | metalloendopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005769 | cellular_component | early endosome |
| A | 0005802 | cellular_component | trans-Golgi network |
| A | 0005813 | cellular_component | centrosome |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005903 | cellular_component | brush border |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0006508 | biological_process | proteolysis |
| A | 0006518 | biological_process | peptide metabolic process |
| A | 0007611 | biological_process | learning or memory |
| A | 0008021 | cellular_component | synaptic vesicle |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008238 | molecular_function | exopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0009986 | cellular_component | cell surface |
| A | 0010814 | biological_process | substance P catabolic process |
| A | 0010815 | biological_process | bradykinin catabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016485 | biological_process | protein processing |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019233 | biological_process | sensory perception of pain |
| A | 0030163 | biological_process | protein catabolic process |
| A | 0030324 | biological_process | lung development |
| A | 0030424 | cellular_component | axon |
| A | 0030425 | cellular_component | dendrite |
| A | 0030667 | cellular_component | secretory granule membrane |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0036064 | cellular_component | ciliary basal body |
| A | 0042277 | molecular_function | peptide binding |
| A | 0042447 | biological_process | hormone catabolic process |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0043025 | cellular_component | neuronal cell body |
| A | 0043627 | biological_process | response to estrogen |
| A | 0044306 | cellular_component | neuron projection terminus |
| A | 0045121 | cellular_component | membrane raft |
| A | 0045202 | cellular_component | synapse |
| A | 0046449 | biological_process | creatinine metabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050435 | biological_process | amyloid-beta metabolic process |
| A | 0050769 | biological_process | positive regulation of neurogenesis |
| A | 0061837 | biological_process | neuropeptide processing |
| A | 0070012 | molecular_function | oligopeptidase activity |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0071345 | biological_process | cellular response to cytokine stimulus |
| A | 0071492 | biological_process | cellular response to UV-A |
| A | 0071493 | biological_process | cellular response to UV-B |
| A | 0090399 | biological_process | replicative senescence |
| A | 0097242 | biological_process | amyloid-beta clearance |
| A | 0098793 | cellular_component | presynapse |
| A | 0150094 | biological_process | amyloid-beta clearance by cellular catabolic process |
| A | 1900273 | biological_process | positive regulation of long-term synaptic potentiation |
| A | 1901612 | molecular_function | cardiolipin binding |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VIGHEITHGF |
| Chain | Residue | Details |
| A | VAL580-PHE589 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 694 |
| Details | Domain: {"description":"Peptidase M13","evidences":[{"source":"PROSITE-ProRule","id":"PRU01233","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01233","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU01233","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8168535","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P07861","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01233","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"10669592","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14747736","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17704566","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"10669592","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14747736","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17704566","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"10669592","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14747736","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17704566","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22766194","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1r1j |
| Chain | Residue | Details |
| A | GLU584 | |
| A | ARG717 | |
| A | HIS711 | |
| A | ASP650 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 623 |
| Chain | Residue | Details |
| A | HIS583 | metal ligand |
| A | GLU584 | proton shuttle (general acid/base) |
| A | HIS587 | metal ligand |
| A | GLU646 | metal ligand |
| A | ASP650 | electrostatic stabiliser |
| A | HIS711 | electrostatic stabiliser |
| A | ARG717 | electrostatic stabiliser |
