1R1I

STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-07
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	P 32 2 1
Unit cell lengths	108.845, 108.845, 113.071
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.28015
Rwork	0.276
R-free	0.35835
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.491
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.750
High resolution limit [Å]	2.550	2.550
Rmerge	0.066	0.272
Number of reflections	25414
<I/σ(I)>	6.6
Completeness [%]	99.2	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1