1PKE
Crystal Structure of E. coli purine nucleoside phosphorylase complexed with 2-fluoro-2'-deoxyadenosine and sulfate/phosphate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| A | 0006152 | biological_process | purine nucleoside catabolic process |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0009164 | biological_process | nucleoside catabolic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0042278 | biological_process | purine nucleoside metabolic process |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| B | 0006152 | biological_process | purine nucleoside catabolic process |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0009164 | biological_process | nucleoside catabolic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0042278 | biological_process | purine nucleoside metabolic process |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| C | 0006152 | biological_process | purine nucleoside catabolic process |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0009164 | biological_process | nucleoside catabolic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0042278 | biological_process | purine nucleoside metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 A 449 |
| Chain | Residue |
| A | GLY20 |
| A | ARG43 |
| A | ARG87 |
| A | GLY89 |
| A | SER90 |
| A | 2FD446 |
| A | HOH454 |
| site_id | AC2 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE 2FD A 446 |
| Chain | Residue |
| A | SER90 |
| A | CYS91 |
| A | GLY92 |
| A | PHE159 |
| A | VAL178 |
| A | GLU179 |
| A | MET180 |
| A | GLU181 |
| A | ASP204 |
| A | PO4449 |
| A | HOH538 |
| A | HOH544 |
| A | HIS4 |
| A | ARG43 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 B 450 |
| Chain | Residue |
| B | GLY20 |
| B | ARG87 |
| B | GLY89 |
| B | SER90 |
| B | 2FD447 |
| B | HOH547 |
| C | ARG43 |
| site_id | AC4 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE 2FD B 447 |
| Chain | Residue |
| B | MET64 |
| B | SER90 |
| B | CYS91 |
| B | GLY92 |
| B | PHE159 |
| B | VAL178 |
| B | MET180 |
| B | GLU181 |
| B | ASP204 |
| B | PO4450 |
| B | HOH470 |
| B | HOH498 |
| C | HIS4 |
| C | ARG43 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 C 451 |
| Chain | Residue |
| B | ARG43 |
| C | GLY20 |
| C | ARG24 |
| C | ARG87 |
| C | GLY89 |
| C | SER90 |
| C | 2FD448 |
| site_id | AC6 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE 2FD C 448 |
| Chain | Residue |
| B | HIS4 |
| B | ARG43 |
| B | HOH469 |
| C | SER90 |
| C | CYS91 |
| C | GLY92 |
| C | PHE159 |
| C | VAL178 |
| C | GLU179 |
| C | MET180 |
| C | GLU181 |
| C | ASP204 |
| C | PO4451 |
Functional Information from PROSITE/UniProt
| site_id | PS01232 |
| Number of Residues | 16 |
| Details | PNP_UDP_1 Purine and other phosphorylases family 1 signature. GhGMGiPScSIytkEL |
| Chain | Residue | Details |
| A | GLY61-LEU76 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01627 |
| Chain | Residue | Details |
| A | ASP204 | |
| B | ASP204 | |
| C | ASP204 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12937174 |
| Chain | Residue | Details |
| A | HIS4 | |
| B | ARG43 | |
| C | HIS4 | |
| C | ARG43 | |
| A | ARG43 | |
| B | HIS4 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 15 |
| Details | BINDING: in other chain => ECO:0000269|PubMed:12937174 |
| Chain | Residue | Details |
| A | GLY20 | |
| A | GLU179 | |
| A | SER203 | |
| B | GLY20 | |
| B | ARG24 | |
| B | ARG87 | |
| B | GLU179 | |
| B | SER203 | |
| C | GLY20 | |
| C | ARG24 | |
| C | ARG87 | |
| C | GLU179 | |
| C | SER203 | |
| A | ARG24 | |
| A | ARG87 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | SITE: Important for catalytic activity => ECO:0000255|HAMAP-Rule:MF_01627 |
| Chain | Residue | Details |
| A | ARG217 | |
| B | ARG217 | |
| C | ARG217 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000255|HAMAP-Rule:MF_01627 |
| Chain | Residue | Details |
| A | LYS26 | |
| B | LYS26 | |
| C | LYS26 |
