1OFN
Purification, crystallisation and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis; the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000271 | biological_process | polysaccharide biosynthetic process |
| A | 0005829 | cellular_component | cytosol |
| A | 0008830 | molecular_function | dTDP-4-dehydrorhamnose 3,5-epimerase activity |
| A | 0016854 | molecular_function | racemase and epimerase activity |
| A | 0019305 | biological_process | dTDP-rhamnose biosynthetic process |
| B | 0000271 | biological_process | polysaccharide biosynthetic process |
| B | 0005829 | cellular_component | cytosol |
| B | 0008830 | molecular_function | dTDP-4-dehydrorhamnose 3,5-epimerase activity |
| B | 0016854 | molecular_function | racemase and epimerase activity |
| B | 0019305 | biological_process | dTDP-rhamnose biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A1203 |
| Chain | Residue |
| A | PHE19 |
| A | ASP21 |
| A | ARG23 |
| A | GLY24 |
| A | LEU25 |
| A | LEU26 |
| B | ARG60 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL A1204 |
| Chain | Residue |
| A | MET134 |
| A | HOH2280 |
| A | HOH2281 |
| B | TYR76 |
| B | SER132 |
| B | MET134 |
| B | HOH2128 |
| B | HOH2129 |
| A | THR49 |
| A | SER132 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A1205 |
| Chain | Residue |
| A | HOH2001 |
| A | HOH2002 |
| A | HOH2283 |
| A | HOH2284 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B1203 |
| Chain | Residue |
| B | ILE50 |
| B | HIS63 |
| B | LYS73 |
| B | HIS120 |
| B | TYR133 |
| B | HOH2259 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1dzr |
| Chain | Residue | Details |
| A | ASP170 | |
| A | HIS63 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1dzr |
| Chain | Residue | Details |
| B | ASP170 | |
| B | HIS63 |
