1OFN

Purification, crystallisation and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis; the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway.

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.5 Å)

Cell axes98.57571.99657.100
Cell angles90.0090.0090.00
SpacegroupP 21 21 2
Resolution limits57.74 - 1.50
the highest resolution shell value1.540 - 1.500
R-factor0.146
R-work0.14400
the highest resolution shell value0.171
R-free0.17800
the highest resolution shell value0.220
RMSD bond length0.018
RMSD bond angle1.710

Data Collection Statistics

Resolution limits57.74 - 1.50
the highest resolution shell value -
Number of reflections65259
Rmerge_l_obs0.056
the highest resolution shell value0.263
Completeness100.0
Redundancy4.5
the highest resolution shell value4.1

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, SITTING DROP8

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*