1O6K

Structure of activated form of PKB kinase domain S474D with GSK3 peptide and AMP-PNP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN A1481

Chain	Residue
A	ASN280
A	ASP293
A	ANP1480
A	HOH2265

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN A1482

Chain	Residue
A	ASP293
A	ANP1480
A	HOH2266
A	HOH2267

---

site_id	AC3
Number of Residues	28
Details	BINDING SITE FOR RESIDUE ANP A1480

Chain	Residue
A	GLY161
A	VAL166
A	ALA179
A	LYS181
A	THR213
A	MET229
A	GLU230
A	ALA232
A	GLU236
A	LYS277
A	GLU279
A	ASN280
A	MET282
A	THR292
A	ASP293
A	PHE439
A	MN1481
A	MN1482
A	HOH2008
A	HOH2013
A	HOH2264
A	HOH2265
A	HOH2267
C	ARG6
C	THR8
C	SER9
C	HOH2008
A	GLY159

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	34
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGKGTFGKVIlVrekatgryyamkilrkevIIAK

Chain	Residue	Details
A	LEU158-LYS191

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvYrDIKleNLML

Chain	Residue	Details
A	VAL271-LEU283

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12434148","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by PDPK1","evidences":[{"source":"PubMed","id":"12434148","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15890450","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20059950","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9512493","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"15890450","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20059950","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"O-linked (GlcNAc) threonine","evidences":[{"source":"UniProtKB","id":"P31749","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Glycosylation: {"description":"O-linked (GlcNAc) serine; alternate","evidences":[{"source":"UniProtKB","id":"P31750","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by PKB/AKT1, RPS6KA3, SGK3 and NME7","evidences":[{"source":"PubMed","id":"12054501","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16484495","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20937854","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24391509","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25169422","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34764205","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35606353","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8250835","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	GLU279
A	ASP275

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS277
A	ASP275

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	THR313
A	LYS277
A	ASP275

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASN280
A	LYS277
A	ASP275

---