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1NXK

Crystal structure of staurosporine bound to MAP KAP kinase 2

Functional Information from GO Data
ChainGOidnamespacecontents
A0000165biological_processMAPK cascade
A0000166molecular_functionnucleotide binding
A0002224biological_processtoll-like receptor signaling pathway
A0004672molecular_functionprotein kinase activity
A0004674molecular_functionprotein serine/threonine kinase activity
A0004683molecular_functioncalmodulin-dependent protein kinase activity
A0005515molecular_functionprotein binding
A0005516molecular_functioncalmodulin binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005813cellular_componentcentrosome
A0005829cellular_componentcytosol
A0006468biological_processprotein phosphorylation
A0006691biological_processleukotriene metabolic process
A0006954biological_processinflammatory response
A0006974biological_processDNA damage response
A0009931molecular_functioncalcium-dependent protein serine/threonine kinase activity
A0010803biological_processregulation of tumor necrosis factor-mediated signaling pathway
A0016301molecular_functionkinase activity
A0016310biological_processphosphorylation
A0016740molecular_functiontransferase activity
A0018105biological_processpeptidyl-serine phosphorylation
A0032496biological_processresponse to lipopolysaccharide
A0032675biological_processregulation of interleukin-6 production
A0032680biological_processregulation of tumor necrosis factor production
A0032760biological_processpositive regulation of tumor necrosis factor production
A0034097biological_processresponse to cytokine
A0035556biological_processintracellular signal transduction
A0035924biological_processcellular response to vascular endothelial growth factor stimulus
A0038066biological_processp38MAPK cascade
A0043488biological_processregulation of mRNA stability
A0044351biological_processmacropinocytosis
A0048010biological_processvascular endothelial growth factor receptor signaling pathway
A0048255biological_processmRNA stabilization
A0048839biological_processinner ear development
A0051019molecular_functionmitogen-activated protein kinase binding
A0060907biological_processpositive regulation of macrophage cytokine production
A0070062cellular_componentextracellular exosome
A0070935biological_process3'-UTR-mediated mRNA stabilization
A0106310molecular_functionprotein serine kinase activity
A1900034biological_processregulation of cellular response to heat
B0000165biological_processMAPK cascade
B0000166molecular_functionnucleotide binding
B0002224biological_processtoll-like receptor signaling pathway
B0004672molecular_functionprotein kinase activity
B0004674molecular_functionprotein serine/threonine kinase activity
B0004683molecular_functioncalmodulin-dependent protein kinase activity
B0005515molecular_functionprotein binding
B0005516molecular_functioncalmodulin binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005813cellular_componentcentrosome
B0005829cellular_componentcytosol
B0006468biological_processprotein phosphorylation
B0006691biological_processleukotriene metabolic process
B0006954biological_processinflammatory response
B0006974biological_processDNA damage response
B0009931molecular_functioncalcium-dependent protein serine/threonine kinase activity
B0010803biological_processregulation of tumor necrosis factor-mediated signaling pathway
B0016301molecular_functionkinase activity
B0016310biological_processphosphorylation
B0016740molecular_functiontransferase activity
B0018105biological_processpeptidyl-serine phosphorylation
B0032496biological_processresponse to lipopolysaccharide
B0032675biological_processregulation of interleukin-6 production
B0032680biological_processregulation of tumor necrosis factor production
B0032760biological_processpositive regulation of tumor necrosis factor production
B0034097biological_processresponse to cytokine
B0035556biological_processintracellular signal transduction
B0035924biological_processcellular response to vascular endothelial growth factor stimulus
B0038066biological_processp38MAPK cascade
B0043488biological_processregulation of mRNA stability
B0044351biological_processmacropinocytosis
B0048010biological_processvascular endothelial growth factor receptor signaling pathway
B0048255biological_processmRNA stabilization
B0048839biological_processinner ear development
B0051019molecular_functionmitogen-activated protein kinase binding
B0060907biological_processpositive regulation of macrophage cytokine production
B0070062cellular_componentextracellular exosome
B0070935biological_process3'-UTR-mediated mRNA stabilization
B0106310molecular_functionprotein serine kinase activity
B1900034biological_processregulation of cellular response to heat
C0000165biological_processMAPK cascade
C0000166molecular_functionnucleotide binding
C0002224biological_processtoll-like receptor signaling pathway
C0004672molecular_functionprotein kinase activity
C0004674molecular_functionprotein serine/threonine kinase activity
C0004683molecular_functioncalmodulin-dependent protein kinase activity
C0005515molecular_functionprotein binding
C0005516molecular_functioncalmodulin binding
C0005524molecular_functionATP binding
C0005634cellular_componentnucleus
C0005654cellular_componentnucleoplasm
C0005737cellular_componentcytoplasm
C0005813cellular_componentcentrosome
C0005829cellular_componentcytosol
C0006468biological_processprotein phosphorylation
C0006691biological_processleukotriene metabolic process
C0006954biological_processinflammatory response
C0006974biological_processDNA damage response
C0009931molecular_functioncalcium-dependent protein serine/threonine kinase activity
C0010803biological_processregulation of tumor necrosis factor-mediated signaling pathway
C0016301molecular_functionkinase activity
C0016310biological_processphosphorylation
C0016740molecular_functiontransferase activity
C0018105biological_processpeptidyl-serine phosphorylation
C0032496biological_processresponse to lipopolysaccharide
C0032675biological_processregulation of interleukin-6 production
C0032680biological_processregulation of tumor necrosis factor production
C0032760biological_processpositive regulation of tumor necrosis factor production
C0034097biological_processresponse to cytokine
C0035556biological_processintracellular signal transduction
C0035924biological_processcellular response to vascular endothelial growth factor stimulus
C0038066biological_processp38MAPK cascade
C0043488biological_processregulation of mRNA stability
C0044351biological_processmacropinocytosis
C0048010biological_processvascular endothelial growth factor receptor signaling pathway
C0048255biological_processmRNA stabilization
C0048839biological_processinner ear development
C0051019molecular_functionmitogen-activated protein kinase binding
C0060907biological_processpositive regulation of macrophage cytokine production
C0070062cellular_componentextracellular exosome
C0070935biological_process3'-UTR-mediated mRNA stabilization
C0106310molecular_functionprotein serine kinase activity
C1900034biological_processregulation of cellular response to heat
D0000165biological_processMAPK cascade
D0000166molecular_functionnucleotide binding
D0002224biological_processtoll-like receptor signaling pathway
D0004672molecular_functionprotein kinase activity
D0004674molecular_functionprotein serine/threonine kinase activity
D0004683molecular_functioncalmodulin-dependent protein kinase activity
D0005515molecular_functionprotein binding
D0005516molecular_functioncalmodulin binding
D0005524molecular_functionATP binding
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005829cellular_componentcytosol
D0006468biological_processprotein phosphorylation
D0006691biological_processleukotriene metabolic process
D0006954biological_processinflammatory response
D0006974biological_processDNA damage response
D0009931molecular_functioncalcium-dependent protein serine/threonine kinase activity
D0010803biological_processregulation of tumor necrosis factor-mediated signaling pathway
D0016301molecular_functionkinase activity
D0016310biological_processphosphorylation
D0016740molecular_functiontransferase activity
D0018105biological_processpeptidyl-serine phosphorylation
D0032496biological_processresponse to lipopolysaccharide
D0032675biological_processregulation of interleukin-6 production
D0032680biological_processregulation of tumor necrosis factor production
D0032760biological_processpositive regulation of tumor necrosis factor production
D0034097biological_processresponse to cytokine
D0035556biological_processintracellular signal transduction
D0035924biological_processcellular response to vascular endothelial growth factor stimulus
D0038066biological_processp38MAPK cascade
D0043488biological_processregulation of mRNA stability
D0044351biological_processmacropinocytosis
D0048010biological_processvascular endothelial growth factor receptor signaling pathway
D0048255biological_processmRNA stabilization
D0048839biological_processinner ear development
D0051019molecular_functionmitogen-activated protein kinase binding
D0060907biological_processpositive regulation of macrophage cytokine production
D0070062cellular_componentextracellular exosome
D0070935biological_process3'-UTR-mediated mRNA stabilization
D0106310molecular_functionprotein serine kinase activity
D1900034biological_processregulation of cellular response to heat
Functional Information from PDB Data
site_idAC1
Number of Residues3
DetailsBINDING SITE FOR RESIDUE SO4 B 401
ChainResidue
BTHR195
BSER196
BARG198

site_idAC2
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 B 402
ChainResidue
AGLN53
BGLN53
BLYS56
CGLN53

site_idAC3
Number of Residues15
DetailsBINDING SITE FOR RESIDUE STU A 401
ChainResidue
ALEU72
AGLY73
AVAL78
AALA91
AMSE138
AGLU139
ACYS140
ALEU141
AGLU190
AASN191
ALEU193
ATHR206
AASP207
ALEU70
AGLY71

site_idAC4
Number of Residues15
DetailsBINDING SITE FOR RESIDUE STU B 403
ChainResidue
BLEU70
BGLY71
BLEU72
BGLY73
BVAL78
BALA91
BMSE138
BGLU139
BCYS140
BLEU141
BGLU190
BASN191
BLEU193
BTHR206
BASP207

site_idAC5
Number of Residues16
DetailsBINDING SITE FOR RESIDUE STU C 401
ChainResidue
BASN200
CLEU70
CGLY71
CLEU72
CGLY73
CVAL78
CALA91
CMSE138
CGLU139
CCYS140
CLEU141
CGLU145
CGLU190
CLEU193
CTHR206
CASP207

site_idAC6
Number of Residues13
DetailsBINDING SITE FOR RESIDUE STU D 401
ChainResidue
DLEU70
DGLY71
DLEU72
DGLY73
DVAL78
DALA91
DMSE138
DGLU139
DCYS140
DLEU141
DASN191
DTHR206
DASP207

Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues24
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGLGINGKVLqIfnkrtqek..........FALK
ChainResidueDetails
ALEU70-LYS93

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IaHrDVKpeNLLY
ChainResidueDetails
AILE182-TYR194

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: Proton acceptor
ChainResidueDetails
AASP186
BASP186
CASP186
DASP186

site_idSWS_FT_FI2
Number of Residues8
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159
ChainResidueDetails
ALEU70
ALYS93
BLEU70
BLYS93
CLEU70
CLYS93
DLEU70
DLYS93

site_idSWS_FT_FI3
Number of Residues4
DetailsBINDING:
ChainResidueDetails
AGLU139
BGLU139
CGLU139
DGLU139

site_idSWS_FT_FI4
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:8846784
ChainResidueDetails
ASER9
BSER9
CSER9
DSER9

site_idSWS_FT_FI5
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0000269|PubMed:8846784
ChainResidueDetails
ATHR25
BTHR25
CTHR25
DTHR25

site_idSWS_FT_FI6
Number of Residues4
DetailsMOD_RES: Phosphothreonine; by MAPK14 => ECO:0000269|PubMed:8846784
ChainResidueDetails
ATHR222
BTHR222
CTHR222
DTHR222

site_idSWS_FT_FI7
Number of Residues4
DetailsMOD_RES: Phosphoserine; by MAPK14 => ECO:0000269|PubMed:8846784
ChainResidueDetails
ASER272
BSER272
CSER272
DSER272

site_idSWS_FT_FI8
Number of Residues4
DetailsMOD_RES: Phosphoserine; by autocatalysis => ECO:0000250
ChainResidueDetails
ASER328
BSER328
CSER328
DSER328

site_idSWS_FT_FI9
Number of Residues4
DetailsMOD_RES: Phosphothreonine; by MAPK14 => ECO:0000269|PubMed:8846784, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569
ChainResidueDetails
ATHR334
BTHR334
CTHR334
DTHR334

site_idSWS_FT_FI10
Number of Residues8
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) => ECO:0000269|PubMed:21131586
ChainResidueDetails
ALYS353
BLYS353
CLYS353
DLYS353

218853

PDB entries from 2024-04-24

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