Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1NXK

Crystal structure of staurosporine bound to MAP KAP kinase 2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000165	biological_process	MAPK cascade
A	0000166	molecular_function	nucleotide binding
A	0002224	biological_process	toll-like receptor signaling pathway
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004683	molecular_function	calcium/calmodulin-dependent protein kinase activity
A	0005515	molecular_function	protein binding
A	0005516	molecular_function	calmodulin binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0006468	biological_process	protein phosphorylation
A	0006691	biological_process	leukotriene metabolic process
A	0006954	biological_process	inflammatory response
A	0006974	biological_process	DNA damage response
A	0009931	molecular_function	calcium-dependent protein serine/threonine kinase activity
A	0010803	biological_process	regulation of tumor necrosis factor-mediated signaling pathway
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0032496	biological_process	response to lipopolysaccharide
A	0032675	biological_process	regulation of interleukin-6 production
A	0032680	biological_process	regulation of tumor necrosis factor production
A	0032760	biological_process	positive regulation of tumor necrosis factor production
A	0034097	biological_process	response to cytokine
A	0035556	biological_process	intracellular signal transduction
A	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
A	0036064	cellular_component	ciliary basal body
A	0038066	biological_process	p38MAPK cascade
A	0043488	biological_process	regulation of mRNA stability
A	0044351	biological_process	macropinocytosis
A	0048010	biological_process	vascular endothelial growth factor receptor signaling pathway
A	0048255	biological_process	mRNA stabilization
A	0048839	biological_process	inner ear development
A	0051019	molecular_function	mitogen-activated protein kinase binding
A	0060907	biological_process	positive regulation of macrophage cytokine production
A	0070062	cellular_component	extracellular exosome
A	0070935	biological_process	3'-UTR-mediated mRNA stabilization
A	0106310	molecular_function	protein serine kinase activity
A	1900034	biological_process	regulation of cellular response to heat
B	0000165	biological_process	MAPK cascade
B	0000166	molecular_function	nucleotide binding
B	0002224	biological_process	toll-like receptor signaling pathway
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004683	molecular_function	calcium/calmodulin-dependent protein kinase activity
B	0005515	molecular_function	protein binding
B	0005516	molecular_function	calmodulin binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005813	cellular_component	centrosome
B	0005829	cellular_component	cytosol
B	0006468	biological_process	protein phosphorylation
B	0006691	biological_process	leukotriene metabolic process
B	0006954	biological_process	inflammatory response
B	0006974	biological_process	DNA damage response
B	0009931	molecular_function	calcium-dependent protein serine/threonine kinase activity
B	0010803	biological_process	regulation of tumor necrosis factor-mediated signaling pathway
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0032496	biological_process	response to lipopolysaccharide
B	0032675	biological_process	regulation of interleukin-6 production
B	0032680	biological_process	regulation of tumor necrosis factor production
B	0032760	biological_process	positive regulation of tumor necrosis factor production
B	0034097	biological_process	response to cytokine
B	0035556	biological_process	intracellular signal transduction
B	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
B	0036064	cellular_component	ciliary basal body
B	0038066	biological_process	p38MAPK cascade
B	0043488	biological_process	regulation of mRNA stability
B	0044351	biological_process	macropinocytosis
B	0048010	biological_process	vascular endothelial growth factor receptor signaling pathway
B	0048255	biological_process	mRNA stabilization
B	0048839	biological_process	inner ear development
B	0051019	molecular_function	mitogen-activated protein kinase binding
B	0060907	biological_process	positive regulation of macrophage cytokine production
B	0070062	cellular_component	extracellular exosome
B	0070935	biological_process	3'-UTR-mediated mRNA stabilization
B	0106310	molecular_function	protein serine kinase activity
B	1900034	biological_process	regulation of cellular response to heat
C	0000165	biological_process	MAPK cascade
C	0000166	molecular_function	nucleotide binding
C	0002224	biological_process	toll-like receptor signaling pathway
C	0004672	molecular_function	protein kinase activity
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0004683	molecular_function	calcium/calmodulin-dependent protein kinase activity
C	0005515	molecular_function	protein binding
C	0005516	molecular_function	calmodulin binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005813	cellular_component	centrosome
C	0005829	cellular_component	cytosol
C	0006468	biological_process	protein phosphorylation
C	0006691	biological_process	leukotriene metabolic process
C	0006954	biological_process	inflammatory response
C	0006974	biological_process	DNA damage response
C	0009931	molecular_function	calcium-dependent protein serine/threonine kinase activity
C	0010803	biological_process	regulation of tumor necrosis factor-mediated signaling pathway
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0032496	biological_process	response to lipopolysaccharide
C	0032675	biological_process	regulation of interleukin-6 production
C	0032680	biological_process	regulation of tumor necrosis factor production
C	0032760	biological_process	positive regulation of tumor necrosis factor production
C	0034097	biological_process	response to cytokine
C	0035556	biological_process	intracellular signal transduction
C	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
C	0036064	cellular_component	ciliary basal body
C	0038066	biological_process	p38MAPK cascade
C	0043488	biological_process	regulation of mRNA stability
C	0044351	biological_process	macropinocytosis
C	0048010	biological_process	vascular endothelial growth factor receptor signaling pathway
C	0048255	biological_process	mRNA stabilization
C	0048839	biological_process	inner ear development
C	0051019	molecular_function	mitogen-activated protein kinase binding
C	0060907	biological_process	positive regulation of macrophage cytokine production
C	0070062	cellular_component	extracellular exosome
C	0070935	biological_process	3'-UTR-mediated mRNA stabilization
C	0106310	molecular_function	protein serine kinase activity
C	1900034	biological_process	regulation of cellular response to heat
D	0000165	biological_process	MAPK cascade
D	0000166	molecular_function	nucleotide binding
D	0002224	biological_process	toll-like receptor signaling pathway
D	0004672	molecular_function	protein kinase activity
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0004683	molecular_function	calcium/calmodulin-dependent protein kinase activity
D	0005515	molecular_function	protein binding
D	0005516	molecular_function	calmodulin binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005813	cellular_component	centrosome
D	0005829	cellular_component	cytosol
D	0006468	biological_process	protein phosphorylation
D	0006691	biological_process	leukotriene metabolic process
D	0006954	biological_process	inflammatory response
D	0006974	biological_process	DNA damage response
D	0009931	molecular_function	calcium-dependent protein serine/threonine kinase activity
D	0010803	biological_process	regulation of tumor necrosis factor-mediated signaling pathway
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0032496	biological_process	response to lipopolysaccharide
D	0032675	biological_process	regulation of interleukin-6 production
D	0032680	biological_process	regulation of tumor necrosis factor production
D	0032760	biological_process	positive regulation of tumor necrosis factor production
D	0034097	biological_process	response to cytokine
D	0035556	biological_process	intracellular signal transduction
D	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
D	0036064	cellular_component	ciliary basal body
D	0038066	biological_process	p38MAPK cascade
D	0043488	biological_process	regulation of mRNA stability
D	0044351	biological_process	macropinocytosis
D	0048010	biological_process	vascular endothelial growth factor receptor signaling pathway
D	0048255	biological_process	mRNA stabilization
D	0048839	biological_process	inner ear development
D	0051019	molecular_function	mitogen-activated protein kinase binding
D	0060907	biological_process	positive regulation of macrophage cytokine production
D	0070062	cellular_component	extracellular exosome
D	0070935	biological_process	3'-UTR-mediated mRNA stabilization
D	0106310	molecular_function	protein serine kinase activity
D	1900034	biological_process	regulation of cellular response to heat

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 401

Chain	Residue
B	THR195
B	SER196
B	ARG198

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 402

Chain	Residue
A	GLN53
B	GLN53
B	LYS56
C	GLN53

site_id	AC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE STU A 401

Chain	Residue
A	LEU72
A	GLY73
A	VAL78
A	ALA91
A	MSE138
A	GLU139
A	CYS140
A	LEU141
A	GLU190
A	ASN191
A	LEU193
A	THR206
A	ASP207
A	LEU70
A	GLY71

site_id	AC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE STU B 403

Chain	Residue
B	LEU70
B	GLY71
B	LEU72
B	GLY73
B	VAL78
B	ALA91
B	MSE138
B	GLU139
B	CYS140
B	LEU141
B	GLU190
B	ASN191
B	LEU193
B	THR206
B	ASP207

site_id	AC5
Number of Residues	16
Details	BINDING SITE FOR RESIDUE STU C 401

Chain	Residue
B	ASN200
C	LEU70
C	GLY71
C	LEU72
C	GLY73
C	VAL78
C	ALA91
C	MSE138
C	GLU139
C	CYS140
C	LEU141
C	GLU145
C	GLU190
C	LEU193
C	THR206
C	ASP207

site_id	AC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE STU D 401

Chain	Residue
D	LEU70
D	GLY71
D	LEU72
D	GLY73
D	VAL78
D	ALA91
D	MSE138
D	GLU139
D	CYS140
D	LEU141
D	ASN191
D	THR206
D	ASP207

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGLGINGKVLqIfnkrtqek..........FALK

Chain	Residue	Details
A	LEU70-LYS93

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IaHrDVKpeNLLY

Chain	Residue	Details
A	ILE182-TYR194

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine; by MAPK14","evidences":[{"source":"PubMed","id":"8846784","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by MAPK14","evidences":[{"source":"PubMed","id":"8846784","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP186
A	GLU190

site_id	CSA10
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP186
A	ASN191
A	LYS188

site_id	CSA11
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
B	ASP186
B	ASN191
B	LYS188

site_id	CSA12
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
C	ASP186
C	ASN191
C	LYS188

site_id	CSA13
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
D	ASP186
D	ASN191
D	LYS188

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
B	ASP186
B	GLU190

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
C	ASP186
C	GLU190

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
D	ASP186
D	GLU190

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP186
A	LYS188

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
B	ASP186
B	LYS188

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
C	ASP186
C	LYS188

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
D	ASP186
D	LYS188

site_id	CSA9
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
D	THR226
D	ASP186
D	LYS188

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)