1KJY
Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001664 | molecular_function | G protein-coupled receptor binding |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0003925 | molecular_function | G protein activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005730 | cellular_component | nucleolus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005813 | cellular_component | centrosome |
| A | 0005829 | cellular_component | cytosol |
| A | 0005834 | cellular_component | heterotrimeric G-protein complex |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005938 | cellular_component | cell cortex |
| A | 0007165 | biological_process | signal transduction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| A | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| A | 0007198 | biological_process | adenylate cyclase-inhibiting serotonin receptor signaling pathway |
| A | 0010854 | molecular_function | adenylate cyclase regulator activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0019003 | molecular_function | GDP binding |
| A | 0030496 | cellular_component | midbody |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| A | 0031749 | molecular_function | D2 dopamine receptor binding |
| A | 0031821 | molecular_function | G protein-coupled serotonin receptor binding |
| A | 0034695 | biological_process | response to prostaglandin E |
| A | 0043434 | biological_process | response to peptide hormone |
| A | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051301 | biological_process | cell division |
| A | 0060236 | biological_process | regulation of mitotic spindle organization |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0072678 | biological_process | T cell migration |
| A | 1904322 | biological_process | cellular response to forskolin |
| A | 1904778 | biological_process | positive regulation of protein localization to cell cortex |
| B | 0005096 | molecular_function | GTPase activator activity |
| B | 0030695 | molecular_function | GTPase regulator activity |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0001664 | molecular_function | G protein-coupled receptor binding |
| C | 0003924 | molecular_function | GTPase activity |
| C | 0003925 | molecular_function | G protein activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005525 | molecular_function | GTP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005730 | cellular_component | nucleolus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005765 | cellular_component | lysosomal membrane |
| C | 0005813 | cellular_component | centrosome |
| C | 0005829 | cellular_component | cytosol |
| C | 0005834 | cellular_component | heterotrimeric G-protein complex |
| C | 0005856 | cellular_component | cytoskeleton |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0005938 | cellular_component | cell cortex |
| C | 0007165 | biological_process | signal transduction |
| C | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| C | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| C | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| C | 0007198 | biological_process | adenylate cyclase-inhibiting serotonin receptor signaling pathway |
| C | 0010854 | molecular_function | adenylate cyclase regulator activity |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0019001 | molecular_function | guanyl nucleotide binding |
| C | 0019003 | molecular_function | GDP binding |
| C | 0030496 | cellular_component | midbody |
| C | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| C | 0031749 | molecular_function | D2 dopamine receptor binding |
| C | 0031821 | molecular_function | G protein-coupled serotonin receptor binding |
| C | 0034695 | biological_process | response to prostaglandin E |
| C | 0043434 | biological_process | response to peptide hormone |
| C | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051301 | biological_process | cell division |
| C | 0060236 | biological_process | regulation of mitotic spindle organization |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0072678 | biological_process | T cell migration |
| C | 1904322 | biological_process | cellular response to forskolin |
| C | 1904778 | biological_process | positive regulation of protein localization to cell cortex |
| D | 0005096 | molecular_function | GTPase activator activity |
| D | 0030695 | molecular_function | GTPase regulator activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 800 |
| Chain | Residue |
| A | GDP356 |
| B | SER510 |
| B | HIS513 |
| B | ARG516 |
| B | HOH4085 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG C 801 |
| Chain | Residue |
| C | GDP355 |
| C | SER1047 |
| C | ASP1200 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS C 802 |
| Chain | Residue |
| C | SER1143 |
| C | PHE1140 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS A 803 |
| Chain | Residue |
| A | PHE140 |
| A | SER143 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CS A 804 |
| Chain | Residue |
| A | ASP158 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS C 805 |
| Chain | Residue |
| C | HIS1057 |
| C | THR1190 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS C 806 |
| Chain | Residue |
| C | ASN1157 |
| C | ASP1158 |
| site_id | AC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CS D 807 |
| Chain | Residue |
| D | ASP1496 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS A 808 |
| Chain | Residue |
| A | ALA59 |
| A | TYR61 |
| site_id | BC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS C 809 |
| Chain | Residue |
| C | LYS1092 |
| C | ASP1094 |
| site_id | BC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CS A 811 |
| Chain | Residue |
| A | HIS188 |
| site_id | BC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS A 812 |
| Chain | Residue |
| A | HIS57 |
| A | THR190 |
| site_id | BC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CS A 813 |
| Chain | Residue |
| A | GLU186 |
| site_id | BC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CS C 814 |
| Chain | Residue |
| C | HIS1188 |
| site_id | BC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS C 815 |
| Chain | Residue |
| C | GLU1186 |
| C | HOH4001 |
| site_id | BC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CS A 816 |
| Chain | Residue |
| A | PHE95 |
| A | GLY96 |
| site_id | BC8 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE GDP C 355 |
| Chain | Residue |
| C | MG801 |
| C | GLY1042 |
| C | GLU1043 |
| C | SER1044 |
| C | GLY1045 |
| C | LYS1046 |
| C | SER1047 |
| C | THR1048 |
| C | SER1151 |
| C | ARG1176 |
| C | ARG1178 |
| C | ASN1269 |
| C | LYS1270 |
| C | ASP1272 |
| C | LEU1273 |
| C | CYS1325 |
| C | ALA1326 |
| C | THR1327 |
| C | HOH4178 |
| D | ARG1516 |
| site_id | BC9 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE GDP A 356 |
| Chain | Residue |
| A | GLY42 |
| A | GLU43 |
| A | SER44 |
| A | GLY45 |
| A | LYS46 |
| A | SER47 |
| A | THR48 |
| A | SER151 |
| A | ARG176 |
| A | ARG178 |
| A | ASN269 |
| A | LYS270 |
| A | ASP272 |
| A | LEU273 |
| A | CYS325 |
| A | ALA326 |
| A | THR327 |
| A | HOH4043 |
| B | ARG516 |
| B | MG800 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:21115486, ECO:0007744|PDB:1KJY, ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | SER44 | |
| A | LYS270 | |
| C | SER1044 | |
| C | LYS1270 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:18434541, ECO:0000269|PubMed:22383884, ECO:0007744|PDB:3QE0 |
| Chain | Residue | Details |
| A | THR48 | |
| A | THR182 | |
| C | THR1048 | |
| C | THR1182 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | ALA152 | |
| C | ALA1152 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | ARG176 | |
| C | ARG1176 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:21115486 |
| Chain | Residue | Details |
| A | VAL201 | |
| C | VAL1201 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q |
| Chain | Residue | Details |
| A | THR327 | |
| C | THR1327 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250 |
| Chain | Residue | Details |
| A | VAL179 | |
| C | VAL1179 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | MOD_RES: Deamidated glutamine; by Photorhabdus PAU_02230 => ECO:0000269|PubMed:24141704 |
| Chain | Residue | Details |
| A | ARG205 | |
| C | ARG1205 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylcysteine; by pertussis toxin => ECO:0000250 |
| Chain | Residue | Details |
| A | GLY352 | |
| C | GLY1352 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| A | THR181 | |
| A | GLN204 | |
| A | GLU43 | |
| A | ARG178 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| C | GLU1043 | |
| C | GLN1204 | |
| C | ARG1178 | |
| C | THR1181 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| A | GLN204 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| C | GLN1204 |
