1KJY
Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 69.510, 82.040, 187.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.238 * |
| Rwork | 0.238 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bof |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.370 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.111 | 0.480 |
| Total number of observations | 136468 * | |
| Number of reflections | 29900 * | 2875 * |
| <I/σ(I)> | 14 | 3.4 |
| Completeness [%] | 99.1 | 97.5 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 18 * | Cesium Sulfate, sodium acetate, glycerol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | and 1.5-fold molar excess R14GL peptide |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | GDP | 0.010 (mM) | |
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 7 | 1 | reservoir | glycerol | 10 (%) | |
| 8 | 1 | reservoir | caesium sulphate | 1.4 (M) |
