1JL0
Structure of a Human S-Adenosylmethionine Decarboxylase Self-processing Ester Intermediate and Mechanism of Putrescine Stimulation of Processing as Revealed by the H243A Mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004014 | molecular_function | adenosylmethionine decarboxylase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006595 | biological_process | polyamine metabolic process |
| A | 0006596 | biological_process | polyamine biosynthetic process |
| A | 0006597 | biological_process | spermine biosynthetic process |
| A | 0008295 | biological_process | spermidine biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| A | 0019810 | molecular_function | putrescine binding |
| A | 0042802 | molecular_function | identical protein binding |
| B | 0004014 | molecular_function | adenosylmethionine decarboxylase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0006595 | biological_process | polyamine metabolic process |
| B | 0006596 | biological_process | polyamine biosynthetic process |
| B | 0006597 | biological_process | spermine biosynthetic process |
| B | 0008295 | biological_process | spermidine biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016831 | molecular_function | carboxy-lyase activity |
| B | 0019810 | molecular_function | putrescine binding |
| B | 0042802 | molecular_function | identical protein binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PUT A 401 |
| Chain | Residue |
| A | LEU13 |
| A | HOH3394 |
| A | GLU15 |
| A | PHE111 |
| A | ASP174 |
| A | THR176 |
| A | PHE285 |
| A | TYR318 |
| A | HOH3001 |
| A | HOH3018 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE TRS A 402 |
| Chain | Residue |
| A | HIS5 |
| A | PHE7 |
| A | CYS226 |
| A | PRO246 |
| A | GLU247 |
| A | HOH3075 |
| A | HOH3220 |
| A | HOH3361 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PUT B 401 |
| Chain | Residue |
| B | LEU13 |
| B | GLU15 |
| B | PHE111 |
| B | ASP174 |
| B | THR176 |
| B | TYR318 |
| B | HOH503 |
| B | HOH504 |
| B | HOH514 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE TRS B 402 |
| Chain | Residue |
| B | HIS5 |
| B | CYS226 |
| B | PRO246 |
| B | GLU247 |
| B | HOH587 |
| B | HOH733 |
Functional Information from PROSITE/UniProt
| site_id | PS01336 |
| Number of Residues | 11 |
| Details | ADOMETDC S-adenosylmethionine decarboxylase signature. AYVLSESSMFV |
| Chain | Residue | Details |
| A | ALA62-VAL72 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"1917972","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Schiff-base intermediate with substrate; via pyruvic acid","evidences":[{"source":"PubMed","id":"11583147","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor; for catalytic activity","evidences":[{"source":"PubMed","id":"10029540","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11583147","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor; for processing activity","evidences":[{"source":"PubMed","id":"11583147","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11583147","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Cleavage (non-hydrolytic); by autolysis","evidences":[{"source":"PubMed","id":"2460457","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"2687270","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Pyruvic acid (Ser); by autocatalysis","evidences":[{"source":"PubMed","id":"2460457","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"2687270","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1jen |
| Chain | Residue | Details |
| A | ALA243 | |
| A | SER229 | |
| A | CYS82 | |
| B | GLU11 | |
| B | GLU67 |
| site_id | CSA2 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1jen |
| Chain | Residue | Details |
| A | GLU11 | |
| A | GLU67 | |
| B | SER229 | |
| B | ALA243 | |
| B | CYS82 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 575 |
| Chain | Residue | Details |
| A | GLU67 | |
| A | SER68 | covalently attached, electron shuttle |
| A | CYS82 | proton shuttle (general acid/base) |
| A | SER229 | |
| A | ALA243 |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 575 |
| Chain | Residue | Details |
| B | GLU67 | |
| B | SER68 | covalently attached, electron shuttle |
| B | CYS82 | proton shuttle (general acid/base) |
| B | SER229 | |
| B | ALA243 |
