1JL0
Structure of a Human S-Adenosylmethionine Decarboxylase Self-processing Ester Intermediate and Mechanism of Putrescine Stimulation of Processing as Revealed by the H243A Mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004014 | molecular_function | adenosylmethionine decarboxylase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006595 | biological_process | polyamine metabolic process |
| A | 0006597 | biological_process | spermine biosynthetic process |
| A | 0008295 | biological_process | spermidine biosynthetic process |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| A | 0019810 | molecular_function | putrescine binding |
| A | 0042802 | molecular_function | identical protein binding |
| B | 0004014 | molecular_function | adenosylmethionine decarboxylase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0006595 | biological_process | polyamine metabolic process |
| B | 0006597 | biological_process | spermine biosynthetic process |
| B | 0008295 | biological_process | spermidine biosynthetic process |
| B | 0016831 | molecular_function | carboxy-lyase activity |
| B | 0019810 | molecular_function | putrescine binding |
| B | 0042802 | molecular_function | identical protein binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PUT A 401 |
| Chain | Residue |
| A | LEU13 |
| A | HOH3394 |
| A | GLU15 |
| A | PHE111 |
| A | ASP174 |
| A | THR176 |
| A | PHE285 |
| A | TYR318 |
| A | HOH3001 |
| A | HOH3018 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE TRS A 402 |
| Chain | Residue |
| A | HIS5 |
| A | PHE7 |
| A | CYS226 |
| A | PRO246 |
| A | GLU247 |
| A | HOH3075 |
| A | HOH3220 |
| A | HOH3361 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PUT B 401 |
| Chain | Residue |
| B | LEU13 |
| B | GLU15 |
| B | PHE111 |
| B | ASP174 |
| B | THR176 |
| B | TYR318 |
| B | HOH503 |
| B | HOH504 |
| B | HOH514 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE TRS B 402 |
| Chain | Residue |
| B | HIS5 |
| B | CYS226 |
| B | PRO246 |
| B | GLU247 |
| B | HOH587 |
| B | HOH733 |
Functional Information from PROSITE/UniProt
| site_id | PS01336 |
| Number of Residues | 11 |
| Details | ADOMETDC S-adenosylmethionine decarboxylase signature. AYVLSESSMFV |
| Chain | Residue | Details |
| A | ALA62-VAL72 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:1917972 |
| Chain | Residue | Details |
| A | GLU8 | |
| A | GLU11 | |
| B | GLU8 | |
| B | GLU11 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Schiff-base intermediate with substrate; via pyruvic acid => ECO:0000269|PubMed:11583147 |
| Chain | Residue | Details |
| A | SER68 | |
| B | SER68 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor; for catalytic activity => ECO:0000269|PubMed:10029540, ECO:0000269|PubMed:11583147 |
| Chain | Residue | Details |
| A | CYS82 | |
| B | CYS82 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor; for processing activity => ECO:0000269|PubMed:11583147 |
| Chain | Residue | Details |
| A | SER229 | |
| A | ALA243 | |
| B | SER229 | |
| B | ALA243 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11583147 |
| Chain | Residue | Details |
| A | PHE7 | |
| A | GLU67 | |
| A | PHE223 | |
| A | GLU247 | |
| B | PHE7 | |
| B | GLU67 | |
| B | PHE223 | |
| B | GLU247 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | SITE: Cleavage (non-hydrolytic); by autolysis => ECO:0000269|PubMed:2460457, ECO:0000269|PubMed:2687270 |
| Chain | Residue | Details |
| A | GLU67 | |
| B | GLU67 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Pyruvic acid (Ser); by autocatalysis => ECO:0000269|PubMed:2460457, ECO:0000269|PubMed:2687270 |
| Chain | Residue | Details |
| A | SER68 | |
| B | SER68 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| A | SER298 | |
| B | SER298 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1jen |
| Chain | Residue | Details |
| A | ALA243 | |
| A | SER229 | |
| A | CYS82 | |
| B | GLU11 | |
| B | GLU67 |
| site_id | CSA2 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1jen |
| Chain | Residue | Details |
| A | GLU11 | |
| A | GLU67 | |
| B | SER229 | |
| B | ALA243 | |
| B | CYS82 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 575 |
| Chain | Residue | Details |
| A | GLU67 | |
| A | SER68 | covalently attached, electron shuttle |
| A | CYS82 | proton shuttle (general acid/base) |
| A | SER229 | |
| A | ALA243 |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 575 |
| Chain | Residue | Details |
| B | GLU67 | |
| B | SER68 | covalently attached, electron shuttle |
| B | CYS82 | proton shuttle (general acid/base) |
| B | SER229 | |
| B | ALA243 |
