Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1FQK

CRYSTAL STRUCTURE OF THE HETERODIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0019001	molecular_function	guanyl nucleotide binding
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0008277	biological_process	regulation of G protein-coupled receptor signaling pathway
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
C	0019001	molecular_function	guanyl nucleotide binding
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
D	0008277	biological_process	regulation of G protein-coupled receptor signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 352

Chain	Residue
A	SER43
A	THR177
A	GDP360
A	ALF362
A	HOH364
A	HOH365

site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ALF A 362

Chain	Residue
A	LYS42
A	ARG174
A	VAL175
A	LYS176
A	THR177
A	GLY199
A	GLN200
A	MG352
A	GDP360
A	HOH363
A	HOH364
A	HOH365
A	ALA37
A	GLY38
A	GLU39

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 352

Chain	Residue
C	SER43
C	THR177
C	GDP361
C	ALF363
C	HOH365
C	HOH366

site_id	AC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ALF C 363

Chain	Residue
C	GLY38
C	GLU39
C	LYS42
C	ARG174
C	VAL175
C	LYS176
C	THR177
C	GLY199
C	GLN200
C	MG352
C	GDP361
C	HOH364
C	HOH365
C	HOH366

site_id	AC5
Number of Residues	25
Details	BINDING SITE FOR RESIDUE GDP A 360

Chain	Residue
A	GLY38
A	GLU39
A	SER40
A	GLY41
A	LYS42
A	SER43
A	THR44
A	ASP146
A	SER147
A	LEU171
A	ARG172
A	SER173
A	ARG174
A	ASN265
A	LYS266
A	ASP268
A	LEU269
A	CYS321
A	ALA322
A	THR323
A	MG352
A	ALF362
A	HOH364
A	HOH368
A	HOH382

site_id	AC6
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GDP C 361

Chain	Residue
C	GLY38
C	GLU39
C	SER40
C	GLY41
C	LYS42
C	SER43
C	THR44
C	ASP146
C	SER147
C	LEU171
C	ARG172
C	SER173
C	ARG174
C	ASN265
C	LYS266
C	ASP268
C	CYS321
C	ALA322
C	THR323
C	MG352
C	ALF363
C	HOH365
C	HOH370
C	HOH387

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	18
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	14
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	10
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7969474","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8208289","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8259210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FQJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FQK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GOT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TAD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TAG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TND","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3V00","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19703466","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8259210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TND","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	30
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P11488","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	230
Details	Domain: {"description":"RGS","evidences":[{"source":"PROSITE-ProRule","id":"PRU00171","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	THR177
A	GLN200
A	GLU39
A	ARG174

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	THR177
C	GLN200
C	GLU39
C	ARG174

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	GLN200

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	GLN200

site_id	MCSA1
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
A	GLU39	electrostatic stabiliser
A	THR44	electrostatic stabiliser
A	ARG174	electrostatic stabiliser
A	ASP196	electrostatic stabiliser
A	GLN200	electrostatic stabiliser

site_id	MCSA2
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
C	GLU39	electrostatic stabiliser
C	THR44	electrostatic stabiliser
C	ARG174	electrostatic stabiliser
C	ASP196	electrostatic stabiliser
C	GLN200	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)