Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1EAK

Catalytic domain of proMMP-2 E404Q mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0006508	biological_process	proteolysis
A	0008237	molecular_function	metallopeptidase activity
A	0008270	molecular_function	zinc ion binding
A	0031012	cellular_component	extracellular matrix
B	0004222	molecular_function	metalloendopeptidase activity
B	0006508	biological_process	proteolysis
B	0008237	molecular_function	metallopeptidase activity
B	0008270	molecular_function	zinc ion binding
B	0031012	cellular_component	extracellular matrix
C	0004222	molecular_function	metalloendopeptidase activity
C	0006508	biological_process	proteolysis
C	0008237	molecular_function	metallopeptidase activity
C	0008270	molecular_function	zinc ion binding
C	0031012	cellular_component	extracellular matrix
D	0004222	molecular_function	metalloendopeptidase activity
D	0006508	biological_process	proteolysis
D	0008237	molecular_function	metallopeptidase activity
D	0008270	molecular_function	zinc ion binding
D	0031012	cellular_component	extracellular matrix

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 851

Chain	Residue
A	GLN298
A	GLY299
A	THR300
C	THR307

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 996

Chain	Residue
A	HIS178
A	ASP180
A	HIS193
A	HIS206

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 997

Chain	Residue
A	HIS403
A	HIS407
A	HIS413
A	CYS102

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 998

Chain	Residue
A	ASP185
A	GLY186
A	ASP188
A	LEU190
A	ASP208
A	GLU211

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 999

Chain	Residue
A	ALA167
A	ASP168
A	GLY198
A	GLY200
A	GLY202

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 852

Chain	Residue
B	GLN298
B	GLY299
B	THR300
D	THR307
R	ALA753

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 853

Chain	Residue
B	LYS242
B	TYR244
B	ASN245
B	SER246

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 996

Chain	Residue
B	HIS178
B	ASP180
B	HIS193
B	HIS206

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 997

Chain	Residue
B	CYS102
B	HIS403
B	HIS407
B	HIS413

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 998

Chain	Residue
B	ASP185
B	GLY186
B	ASP188
B	LEU190
B	ASP208
B	GLU211

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA B 999

Chain	Residue
B	ASP168
B	GLY198
B	GLY200
B	GLY202

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 850

Chain	Residue
A	THR307
C	GLN298
C	GLY299
C	THR300
P	ALA753

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 856

Chain	Residue
C	ARG432
C	SER434
C	GLN435

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 996

Chain	Residue
C	HIS178
C	ASP180
C	HIS193
C	HIS206

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 997

Chain	Residue
C	CYS102
C	HIS403
C	HIS407
C	HIS413

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA C 998

Chain	Residue
C	ASP185
C	GLY186
C	ASP188
C	LEU190
C	ASP208
C	GLU211

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA C 999

Chain	Residue
C	ASP168
C	GLY198
C	GLY200
C	GLY202

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 857

Chain	Residue
B	THR307
D	GLY299
D	THR300

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 996

Chain	Residue
D	HIS178
D	ASP180
D	HIS193
D	HIS206

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 997

Chain	Residue
D	CYS102
D	HIS403
D	HIS407
D	HIS413

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA D 998

Chain	Residue
D	ASP185
D	GLY186
D	ASP188
D	LEU190
D	ASP208
D	GLU211

site_id	CC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA D 999

Chain	Residue
D	ALA167
D	ASP168
D	GLY200
D	GLY202

site_id	CC5
Number of Residues	14
Details	BINDING SITE FOR CHAIN P OF INHIBITOR PEPTIDE

Chain	Residue
A	PRO133
A	ASP209
A	HIS276
A	GLU277
A	ALA278
A	PHE293
A	ASP303
A	SER304
C	ARG296
C	GLY299
C	SER301
C	SO4850
A	GLY130
A	TYR131

site_id	CC6
Number of Residues	14
Details	BINDING SITE FOR CHAIN R OF INHIBITOR PEPTIDE

Chain	Residue
B	ARG296
B	GLN298
B	GLY299
B	THR300
B	SER301
B	SO4852
D	PRO133
D	ASP209
D	HIS276
D	GLU277
D	ALA278
D	PHE293
D	SER304
D	THR306

Functional Information from PROSITE/UniProt

site_id	PS00023
Number of Residues	42
Details	FN2_1 Fibronectin type-II collagen-binding domain signature. CkfPFlFngkeynsCtdtgrsdgflWCsttyNFekdgkYgFC

Chain	Residue	Details
A	CYS233-CYS274
A	CYS291-CYS332
A	CYS349-CYS390

site_id	PS00546
Number of Residues	8
Details	CYSTEINE_SWITCH Matrixins cysteine switch. PRCGnPDV

Chain	Residue	Details
A	PRO100-VAL107

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	192
Details	Domain: {"description":"Fibronectin type-II 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00479","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	192
Details	Domain: {"description":"Fibronectin type-II 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00479","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	192
Details	Domain: {"description":"Fibronectin type-II 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00479","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	444
Details	Region: {"description":"Collagenase-like 1"}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	696
Details	Region: {"description":"Collagen-binding"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	28
Details	Motif: {"description":"Cysteine switch","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	4
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10356396","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	4
Details	Binding site: {"description":"in inhibited form","evidences":[{"source":"PubMed","id":"10356396","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12032297","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CK7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EAK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GXD","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21813640","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1QIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AYU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21813640","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1EAK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AYU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10356396","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12032297","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21813640","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CK7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EAK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GXD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AYU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21813640","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3AYU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10356396","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12032297","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21813640","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CK7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EAK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GXD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AYU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
A	MET421
A	GLN404

site_id	CSA10
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
C	GLN404

site_id	CSA11
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
D	LYS224

site_id	CSA12
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
D	GLN404

site_id	CSA13
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
A	GLU231

site_id	CSA14
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
B	GLU231

site_id	CSA15
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
C	GLU231

site_id	CSA16
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
D	GLU231

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
B	MET421
B	GLN404

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
C	MET421
C	GLN404

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
D	MET421
D	GLN404

site_id	CSA5
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
A	LYS224

site_id	CSA6
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
A	GLN404

site_id	CSA7
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
B	LYS224

site_id	CSA8
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
B	GLN404

site_id	CSA9
Number of Residues	1
Details	Annotated By Reference To The Literature 1hfs

Chain	Residue	Details
C	LYS224

site_id	MCSA1
Number of Residues	4
Details	M-CSA 906

Chain	Residue	Details
A	HIS403	metal ligand
A	GLN404	proton shuttle (general acid/base)
A	HIS407	metal ligand
A	HIS413	metal ligand

site_id	MCSA2
Number of Residues	4
Details	M-CSA 906

Chain	Residue	Details
B	HIS403	metal ligand
B	GLN404	proton shuttle (general acid/base)
B	HIS407	metal ligand
B	HIS413	metal ligand

site_id	MCSA3
Number of Residues	4
Details	M-CSA 906

Chain	Residue	Details
C	HIS403	metal ligand
C	GLN404	proton shuttle (general acid/base)
C	HIS407	metal ligand
C	HIS413	metal ligand

site_id	MCSA4
Number of Residues	4
Details	M-CSA 906

Chain	Residue	Details
D	HIS403	metal ligand
D	GLN404	proton shuttle (general acid/base)
D	HIS407	metal ligand
D	HIS413	metal ligand

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)