1EAK

Catalytic domain of proMMP-2 E404Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE D2AM
Synchrotron site	ESRF
Beamline	D2AM
Temperature [K]	100
Collection date	1997-10-15
Spacegroup name	P 21 21 21
Unit cell lengths	117.670, 166.150, 170.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.580 - 2.660
R-factor	0.271
Rwork	0.271
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ck7
RMSD bond length	0.057
RMSD bond angle	2.300
Data reduction software	XDS
Data scaling software	ROTAPREP
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.500	2.800
High resolution limit [Å]	2.650	2.650
Rmerge	0.093	0.036
Number of reflections	84612
<I/σ(I)>	9.6	0.9
Completeness [%]	93.7	60.8
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		PH 8.50