Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004155 | molecular_function | 6,7-dihydropteridine reductase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006729 | biological_process | tetrahydrobiopterin biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0047040 | molecular_function | pteridine reductase activity |
| B | 0004155 | molecular_function | 6,7-dihydropteridine reductase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006729 | biological_process | tetrahydrobiopterin biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0031427 | biological_process | response to methotrexate |
| B | 0047040 | molecular_function | pteridine reductase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE NDP A 300 |
| Chain | Residue |
| A | ARG17 |
| A | LEU66 |
| A | ASN109 |
| A | ALA110 |
| A | SER111 |
| A | ASP142 |
| A | MET179 |
| A | VAL180 |
| A | ASP181 |
| A | TYR194 |
| A | LYS198 |
| A | LEU18 |
| A | PRO224 |
| A | GLY225 |
| A | LEU226 |
| A | SER227 |
| A | MTX301 |
| A | HOH2005 |
| A | HOH2008 |
| A | HOH2131 |
| A | HOH2253 |
| A | HOH2254 |
| A | HIS36 |
| A | HOH2255 |
| A | HOH2256 |
| A | HOH2257 |
| A | TYR37 |
| A | HIS38 |
| A | ARG39 |
| A | SER40 |
| A | ALA64 |
| A | ASP65 |
| site_id | AC2 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE MTX A 301 |
| Chain | Residue |
| A | ARG17 |
| A | SER111 |
| A | PHE113 |
| A | PRO115 |
| A | LEU188 |
| A | TYR191 |
| A | TYR194 |
| A | LEU226 |
| A | LEU229 |
| A | ASP232 |
| A | MET233 |
| A | NDP300 |
| A | EDO1290 |
| A | HOH2192 |
| A | HOH2198 |
| A | HOH2258 |
| A | HOH2259 |
| A | HOH2260 |
| A | HOH2261 |
| A | HOH2263 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO A 1289 |
| Chain | Residue |
| A | GLY199 |
| A | GLU202 |
| A | ARG206 |
| A | HOH2264 |
| B | GLY199 |
| B | GLU202 |
| B | ARG206 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE EDO A 1290 |
| Chain | Residue |
| A | PHE113 |
| A | ASP181 |
| A | TYR194 |
| A | MTX301 |
| A | HOH2190 |
| A | HOH2265 |
| B | ARG287 |
| B | HOH2198 |
| site_id | AC5 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NDP B 300 |
| Chain | Residue |
| B | ARG17 |
| B | LEU18 |
| B | HIS36 |
| B | TYR37 |
| B | HIS38 |
| B | ARG39 |
| B | SER40 |
| B | ALA64 |
| B | ASP65 |
| B | LEU66 |
| B | ASN109 |
| B | ALA110 |
| B | SER111 |
| B | ASP142 |
| B | MET179 |
| B | VAL180 |
| B | ASP181 |
| B | TYR194 |
| B | LYS198 |
| B | PRO224 |
| B | GLY225 |
| B | SER227 |
| B | MTX301 |
| B | HOH2006 |
| B | HOH2135 |
| B | HOH2203 |
| B | HOH2204 |
| B | HOH2205 |
| B | HOH2206 |
| B | HOH2207 |
| B | HOH2208 |
| site_id | AC6 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE MTX B 301 |
| Chain | Residue |
| B | SER111 |
| B | PHE113 |
| B | PRO115 |
| B | LEU188 |
| B | LEU189 |
| B | TYR191 |
| B | TYR194 |
| B | LEU226 |
| B | HIS241 |
| B | NDP300 |
| B | EDO1289 |
| B | HOH2130 |
| B | HOH2209 |
| B | HOH2210 |
| B | ARG17 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO B 1289 |
| Chain | Residue |
| A | ARG287 |
| B | ASP181 |
| B | MTX301 |
| B | HOH2134 |
| B | HOH2150 |
Functional Information from PROSITE/UniProt
| site_id | PS00061 |
| Number of Residues | 29 |
| Details | ADH_SHORT Short-chain dehydrogenases/reductases family signature. DamtnqpllgYtiYTMAKGALeGLTrSAA |
| Chain | Residue | Details |
| A | ASP181-ALA209 | |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | TYR194 | |
| B | TYR194 | |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | ARG17 | |
| B | ARG17 | |
| Chain | Residue | Details |
| A | SER175 | |
| B | SER175 | |
