1E7W
One active site, two modes of reduction correlate the mechanism of leishmania pteridine reductase with pterin metabolism and antifolate drug resistance in trpanosomes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.87, 0.886, 0.97950, 0.97960 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.312, 80.800, 90.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| Rwork | 0.196 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.032 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 59475 |
| <I/σ(I)> | 24.6 |
| Completeness [%] | 98.2 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | pH 5.40 |
