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1DHF

CRYSTAL STRUCTURES OF RECOMBINANT HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 5-DEAZOFOLATE

Functional Information from GO Data
ChainGOidnamespacecontents
A0000900molecular_functionmRNA regulatory element binding translation repressor activity
A0003723molecular_functionRNA binding
A0003729molecular_functionmRNA binding
A0004146molecular_functiondihydrofolate reductase activity
A0005542molecular_functionfolic acid binding
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005829cellular_componentcytosol
A0006729biological_processtetrahydrobiopterin biosynthetic process
A0006730biological_processone-carbon metabolic process
A0008144molecular_functionobsolete drug binding
A0016491molecular_functionoxidoreductase activity
A0017148biological_processnegative regulation of translation
A0031103biological_processaxon regeneration
A0031427biological_processresponse to methotrexate
A0046452biological_processdihydrofolate metabolic process
A0046653biological_processtetrahydrofolate metabolic process
A0046654biological_processtetrahydrofolate biosynthetic process
A0046655biological_processfolic acid metabolic process
A0050661molecular_functionNADP binding
A0051000biological_processpositive regulation of nitric-oxide synthase activity
A0070402molecular_functionNADPH binding
A1990825molecular_functionsequence-specific mRNA binding
A2000121biological_processregulation of removal of superoxide radicals
B0000900molecular_functionmRNA regulatory element binding translation repressor activity
B0003723molecular_functionRNA binding
B0003729molecular_functionmRNA binding
B0004146molecular_functiondihydrofolate reductase activity
B0005542molecular_functionfolic acid binding
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005829cellular_componentcytosol
B0006729biological_processtetrahydrobiopterin biosynthetic process
B0006730biological_processone-carbon metabolic process
B0008144molecular_functionobsolete drug binding
B0016491molecular_functionoxidoreductase activity
B0017148biological_processnegative regulation of translation
B0031103biological_processaxon regeneration
B0031427biological_processresponse to methotrexate
B0046452biological_processdihydrofolate metabolic process
B0046653biological_processtetrahydrofolate metabolic process
B0046654biological_processtetrahydrofolate biosynthetic process
B0046655biological_processfolic acid metabolic process
B0050661molecular_functionNADP binding
B0051000biological_processpositive regulation of nitric-oxide synthase activity
B0070402molecular_functionNADPH binding
B1990825molecular_functionsequence-specific mRNA binding
B2000121biological_processregulation of removal of superoxide radicals
Functional Information from PDB Data
site_idAC1
Number of Residues16
DetailsBINDING SITE FOR RESIDUE FOL A 187
ChainResidue
AILE7
APRO61
AASN64
ALEU67
AARG70
AVAL115
ATYR121
ATHR136
AVAL8
AALA9
AGLU30
APHE31
AARG32
APHE34
AGLN35
AILE60

site_idAC2
Number of Residues17
DetailsBINDING SITE FOR RESIDUE FOL B 187
ChainResidue
BILE7
BVAL8
BALA9
BGLU30
BPHE31
BARG32
BPHE34
BGLN35
BASN64
BLEU67
BARG70
BVAL115
BTYR121
BTHR136
BHOH401
BHOH402
BHOH410

Functional Information from PROSITE/UniProt
site_idPS00075
Number of Residues24
DetailsDHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGkngdLPWpplrnEfryFqrmT
ChainResidueDetails
AGLY15-THR38

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues10
DetailsBINDING: BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082
ChainResidueDetails
AVAL10
BGLY117
AILE16
ALYS55
AARG77
AGLY117
BVAL10
BILE16
BLYS55
BARG77

site_idSWS_FT_FI2
Number of Residues6
DetailsBINDING: BINDING => ECO:0000305|PubMed:2248959
ChainResidueDetails
APHE31
AARG65
AILE71
BPHE31
BARG65
BILE71

Catalytic Information from CSA
site_idCSA1
Number of Residues2
Detailsa catalytic site defined by CSA, PubMed 8999931, 2244959
ChainResidueDetails
AGLU30
ALEU22

site_idMCSA1
Number of Residues2
DetailsM-CSA 490
ChainResidueDetails
APRO23electrostatic stabiliser
APHE31electrostatic stabiliser

site_idMCSA2
Number of Residues2
DetailsM-CSA 490
ChainResidueDetails
BPRO23electrostatic stabiliser
BPHE31electrostatic stabiliser

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PDB entries from 2024-07-24

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