1DHF
CRYSTAL STRUCTURES OF RECOMBINANT HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 5-DEAZOFOLATE
Experimental procedure
Spacegroup name | P 1 |
Unit cell lengths | 36.100, 38.700, 76.900 |
Unit cell angles | 94.30, 91.70, 111.00 |
Refinement procedure
Resolution | ? - 2.300 |
R-factor | 0.176 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.033 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.300 * |
Rmerge | 0.056 * |
Number of reflections | 16023 * |
Completeness [%] | 91.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.9 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium phosphate | 0.025 (M) | |
2 | 1 | drop | dithioerythritol | 0.5 (mM) | |
3 | 1 | drop | sodium folate | 3-fold molar excess of substrate to enzyme | |
4 | 1 | drop | sodium acetate | 0.30 (M) | |
5 | 1 | drop | PEG4000 | 35 (%(w/v)) | |
6 | 1 | reservoir | sodium acetate | 0.17 (M) | |
7 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
8 | 1 | reservoir | ethanol | 7 (%(v/v)) |