Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070401 | molecular_function | NADP+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 506 |
| site_id | AC2 |
| Number of Residues | 34 |
| Details | BINDING SITE FOR RESIDUE NDP A 500 |
| Chain | Residue |
| A | GLY43 |
| A | ARG44 |
| A | ARG45 |
| A | THR46 |
| A | LEU65 |
| A | SER66 |
| A | ARG67 |
| A | GLN68 |
| A | GLY80 |
| A | ILE94 |
| A | GLY96 |
| A | GLY97 |
| A | GLN98 |
| A | VAL99 |
| A | TYR100 |
| A | LEU102 |
| A | ALA126 |
| A | MTX501 |
| A | GOL503 |
| A | HOH514 |
| A | TRP6 |
| A | HOH519 |
| A | HOH543 |
| A | HOH556 |
| A | HOH571 |
| A | HOH577 |
| A | HOH629 |
| A | HOH648 |
| A | ALA7 |
| A | ILE14 |
| A | GLY15 |
| A | GLY18 |
| A | ASP19 |
| A | ILE20 |
| site_id | AC3 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE MTX A 501 |
| Chain | Residue |
| A | ILE5 |
| A | TRP6 |
| A | ILE20 |
| A | PRO25 |
| A | ASP27 |
| A | GLN28 |
| A | ALA29 |
| A | PHE31 |
| A | ARG32 |
| A | PRO51 |
| A | ARG60 |
| A | ILE94 |
| A | TYR100 |
| A | NDP500 |
| A | GOL502 |
| A | HOH508 |
| A | HOH542 |
| A | HOH559 |
| A | HOH682 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 502 |
| Chain | Residue |
| A | TRP22 |
| A | LEU24 |
| A | ASP27 |
| A | GLN28 |
| A | MTX501 |
| A | HOH609 |
| site_id | AC5 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL A 503 |
| Chain | Residue |
| A | ARG45 |
| A | GLN98 |
| A | GLY124 |
| A | GLY148 |
| A | LEU149 |
| A | NDP500 |
| A | HOH509 |
| A | HOH517 |
| A | HOH574 |
| A | HOH628 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 504 |
| Chain | Residue |
| A | HIS30 |
| A | GLU33 |
| A | ALA52 |
| A | ARG143 |
| A | LEU153 |
| A | HOH552 |
| A | HOH677 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 505 |
| Chain | Residue |
| A | GLN8 |
| A | THR10 |
| A | GLU114 |
| A | TYR154 |
| A | TYR156 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGrggdIPWrlpe.DqahFreiT |
| Chain | Residue | Details |
| A | VAL13-THR35 | |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 25 |
| Details | Binding site: {} |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1dhf |
| Chain | Residue | Details |
| A | ILE20 | |
| A | ASP27 | |
